Article
Biochemistry & Molecular Biology
Megha Shah, Veronique L. Taylor, Diane Bona, Yvonne Tsao, Sabrina Y. Stanley, Sheila M. Pimentel-Elardo, Matthew McCallum, Joseph Bondy-Denomy, P. Lynne Howell, Justin R. Nodwell, Alan R. Davidson, Trevor F. Moraes, Karen L. Maxwell
Summary: The study identified a new phage anti-activator protein, Aqs1, which inhibits phage infection by suppressing the quorum-sensing master regulator LasR. Aqs1 also inhibits the bacterial type IV pilus assembly ATPase protein PilB, preventing phages from superinfecting host cells. This research highlights the potential of small phage proteins to disrupt key biological pathways and offers insights into the evolutionary dynamics between phages and their hosts.
Article
Virology
Guanhua Xuan, Hong Lin, Jingxue Wang
Summary: Research has shown that phage proteins can interfere with host intracellular processes for phage infection, but little is known about proteins that protect the host from phage infection. This study identified a phage protein (Gp21) that can protect Pseudomonas aeruginosa PAO1 from phage infection.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Guanhua Xuan, Hong Lin, Lin Tan, Gang Zhao, Jingxue Wang
Summary: Quorum sensing (QS) plays a crucial role in regulating interactions between bacteria, including phage-bacterium interactions. This study showed that Pseudomonas aeruginosa PAO1 uses las QS to increase phage infection rates by upregulating the expression of galU, which is necessary for synthesizing phage receptor lipopolysaccharides. Inhibition of QS may reduce therapeutic efficacy of phages and affect the balance between antibiotic treatment and phage therapy.
Article
Microbiology
Lan Yang, Tingting Zhang, Linlin Li, Chao Zheng, Demeng Tan, Nannan Wu, Mingyang Wang, Tongyu Zhu
Summary: This study characterizes the bacteriophage L5 as a potential candidate for phage therapy in treating P. aeruginosa infection. The research identifies type IV pili as the phage receptors for L5.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
In-Young Chung, Bi-O Kim, Ju-Hyun Han, Jonggwan Park, Hee Kyoung Kang, Yoonkyung Park, You-Hee Cho
Summary: The study reveals a new method inspired by phages for antibacterial discovery. Critical residues in the bioactivity of the Tip protein were identified, and a synthetic 15-aa peptide P1 showed potential in inhibiting Pseudomonas aeruginosa infections.
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Review
Biochemistry & Molecular Biology
Kayeen Vadakkan, Ajit Kumar Ngangbam, Kuppusamy Sathishkumar, Nelson Pynadathu Rumjit, Meena Kochappan Cheruvathur
Summary: Pseudomonas aeruginosa, a competitive and biofilm organism with complex quorum systems, poses a significant threat to the medical industry. Inhibiting quorum sensing could be a potential strategy for treating bacterial infections and developing anti-virulent tools.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Shouyi Li, Xuetao Gong, Liwen Yin, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Un-Hwan Ha, Weihui Wu
Summary: This study reveals a novel regulatory mechanism in Pseudomonas aeruginosa, where CspC protein regulates the quorum-sensing systems by repressing the translation of a negative regulator gene, rsaL, in response to a host-generated metabolite.
Article
Infectious Diseases
Sahil Shandil, Tsz Tin Yu, Shekh Sabir, David StC. Black, Naresh Kumar
Summary: As bacteria develop resistance against antimicrobials, alternative methods to tackle this issue must be developed. In this study, quinazolinone inhibitors were designed using molecular docking to target the pqs system in P. aeruginosa. Compound 6b showed the highest pqs inhibitory activity and no bacterial growth inhibition.
Article
Microbiology
Guanhua Xuan, Hong Lin, Lin Tan, Gang Zhao, Jingxue Wang
Summary: Quorum sensing (QS) regulates phage-bacterium interactions by upregulating phage receptor expression, increasing phage adsorption and infection rates. This QS-mediated phage susceptibility is a dynamic process depending on host cell density.
Review
Chemistry, Analytical
Yingying Li, Yang Hu, Tao Chen, Yan Chen, Yi Li, Haibo Zhou, Danting Yang
Summary: This review discusses and summarizes advanced detection and sensing strategies for P. aeruginosa cells and its associated quorum sensing molecules over the past ten years. The aim is to achieve rapid, sensitive, and cost-effective diagnosis for infection. The principles of commonly used sensing strategies are briefly introduced, followed by the advancement of these techniques in detecting P. aeruginosa cells and quorum sensing biomarkers. Additionally, the integration of novel platforms for clinical application is highlighted. The current achievements are summarized, and challenges and prospects for the future are proposed.
Article
Microbiology
Pauline Nogaret, Fatima El Garah, Anne-Beatrice Blanc-Potard
Summary: A novel vertebrate animal model using zebrafish was developed to assess drug efficacy against P. aeruginosa, with validation using a clinically used antibiotic and a novel quorum sensing inhibitory molecule. This study provides a new and reliable method for comparing the virulence of P. aeruginosa strains in vivo and evaluating the efficacy of antipseudomonal drugs, including new antivirulence compounds.
Article
Biochemistry & Molecular Biology
Suqi Hao, Dan Yang, Ling Zhao, Fei Shi, Gang Ye, Hualin Fu, Juchun Lin, Hongrui Guo, Ran He, Jianlong Li, Hongwei Chen, Muhammad Faraz Khan, Yinglun Li, Huaqiao Tang
Summary: EGCG, a bioactive ingredient in green tea, demonstrated significant inhibitory effects on the production of virulence factors in Pseudomonas aeruginosa, potentially serving as a candidate for quorum sensing inhibition to combat bacterial infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Rakesh Sikdar, Mikael H. Elias
Summary: Quorum sensing (QS) is a cell-to-cell communication system that regulates bacterial behaviors. Enzymatic quorum quenching (QQ) can interfere with QS and potentially enhance the effects of antibiotics. This study evaluated the impact of QS signal disruption on antibiotic resistance in Pseudomonas aeruginosa and found that it significantly affects resistance. The results highlight the complex relationship between QS and antibiotic resistance and suggest a case-by-case approach to combination therapy.
MICROBIOLOGY SPECTRUM
(2022)