Article
Cell Biology
Noopur Dave, Kaice LaFavers, Gustavo Arrizabalaga
Summary: Calcium signaling plays a crucial role in the regulation of the obligate intracellular parasite Toxoplasma gondii, and a novel EF-hand domain-containing protein, TgEFP1, has been identified in this calcium homeostasis network, localizing to the PLV/ELC and PV. Knockout of TgEFP1 results in faster parasite propagation, hypersensitivity to calcium ionophore-induced egress, and premature natural egress.
Review
Plant Sciences
Hao-ran Liu, Chao Shen, Danial Hassani, Wan-qi Fang, Zhi-yi Wang, Yi Lu, Rui-liang Zhu, Qiong Zhao
Summary: This article summarizes the known knowledge about the morphological, metabolic Constitution properties, and biogenesis mechanism of vacuoles in bryophytes. By comparing the differences in molecular mechanisms among different species and comparing bryophytes with seed plants, a possible mechanism for the biogenesis of vacuoles in bryophytes is proposed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xiao Li, D. Eric Anderson, Yuen-Yan Chang, Michal Jarnik, Matthias P. Machner
Summary: Legionella pneumophila controls vacuole expansion by fine-tuning the generation of lysophospholipids within the vacuolar membrane. Overproduction of VpdC prevents the adequate expansion of the surrounding membrane, trapping the replicating bacteria within spatially confined vacuoles and reducing their capability to proliferate intracellularly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
T. L. Westerman, M. McClelland, J. R. Elfenbein
Summary: Regulation of flagellum biosynthesis is a hierarchical process tightly controlled by the LysR transcriptional regulator YeiE, which promotes virulence by enhancing cell motility. The Delta yeiE mutant lacks motility and shows reduced expression of flagellar class 2 and 3 genes, leading to defective gut colonization. YeiE acts as a control point for flagellar gene expression, adding a new layer to the regulatory cascade for enteric pathogen fitness.
Article
Medicine, Research & Experimental
Haibo Su, Zhongping Liang, ShuFeng Weng, Chaonan Sun, Jiaxin Huang, TianRan Zhang, Xialian Wang, Shanshan Wu, Zhi Zhang, Yiqi Zhang, Qing Gong, Ying Xu
Summary: The research identifies miR-9-5p and IDH3α as critical players in trained immunity, regulating metabolic and epigenetic pathways to control monocytes' response to pathogens.
Article
Microbiology
Fan Qiu, Na Kang, Jinling Tan, Sisi Yan, Leiying Lin, Lipeng Cai, Joel M. Goodman, Qiang Gao
Summary: The ability to sense environmental changes and adjust cellular metabolism is crucial for cell viability. In this study, researchers discovered that Fat1p, a fatty acyl coenzyme A synthetase and fatty acid transporter, plays a key role in maintaining vacuolar shape, regulating vacuolar microdomains, and promoting cell survival in the stationary phase of S. cerevisiae.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Marcel Hahn, Adriana Covarrubias-Pinto, Lina Herhaus, Shankha Satpathy, Kevin Klann, Keith B. Boyle, Christian Munch, Krishnaraj Rajalingam, Felix Randow, Chunaram Choudhary, Ivan Dikic
Summary: SIK2 kinase is a central component of the host defense machinery during Salmonella infection, and its depletion can lead to bacterial escape and impaired Xenophagy. During bacterial infection, SIK2 associates with actin and is recruited to the Salmonella-containing vacuole, controlling the formation of a protective SCV actin shield.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Zhongshun Liu, Congwei Jiang, Zhangmengxue Lei, Sihan Dong, Linlin Kuang, Chenxu Huang, Ying Gao, Mu Liu, Hui Xiao, Patrick Legembre, Jae U. Jung, Huaping Liang, Xiaozhen Liang
Summary: Type I interferons (IFNs) are the first line of defense against invading pathogens. This study identified a previously unknown protein, PINLYP, that interacts with TBK1 to induce the production of type I IFN. Loss of PINLYP impaired the activation of IRF3 and the production of IFN induced by various viruses and Toll-like receptor ligands. Mice lacking PINLYP were more susceptible to lethal virus infection, highlighting the importance of PINLYP in the host defense against viral infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Letter
Biochemistry & Molecular Biology
Oren Shatz, Zvulun Elazar
Summary: Autophagy is a cellular process that selectively eliminates cytoplasmic constituents through engulfment in an isolation membrane, or non-selectively recycles bulk cytoplasm. Autophagosome formation involves the elongation of a newly-formed membrane, called the phagophore, by direct lipid flow from a donor membrane. Recent research has made significant advancements in understanding the regulation of autophagy and autophagosome formation by different lipid species and associated protein complexes. This schematic summary provides an overview of the current understanding of autophagy and autophagosome biogenesis.
Article
Biochemistry & Molecular Biology
Jose L. Nieto-Torres, Sean-Luc Shanahan, Romain Chassefeyre, Tai Chaiamarit, Sviatlana Zaretski, Sara Landeras-Bueno, Adriaan Verhelle, Sandra E. Encalada, Malene Hansen
Summary: Autophagy is a conserved cellular process that promotes homeostasis by degrading cytosolic components. The ATG8 protein family interacts with FYCO1 to regulate the directional transport of autophagosomes.
Article
Multidisciplinary Sciences
Tzu-Huai Lin, Dana M. Bis-Brewer, Amy E. Sheehan, Louise N. Townsend, Daniel C. Maddison, Stephan Zuchner, Gaynor A. Smith, Marc R. Freeman
Summary: This study found that TSG101 inhibits mitochondrial biogenesis in axons, which is crucial for maintaining mitochondrial numbers and sizes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Sean Rogers, Hanaa Hariri, N. Ezgi Wood, Natalie Ortiz Speer, W. Mike Henne
Summary: This study reveals that yeast undergo metabolic remodeling at nucleus-vacuole junctions in response to acute glucose restriction, retaining HMGCRs and promoting mevalonate pathway flux in a spatially coordinated manner. The protein Nvj1 plays a crucial role in this process, demonstrating the importance of inter-organelle contacts in organizing metabolic reactions. Loss of HMGCR compartmentalization perturbs yeast growth following glucose starvation, indicating its role in adaptive metabolic remodeling.
Article
Cell Biology
Ritika Chatterjee, Abhilash Vijay Nair, Anmol Singh, Nishi Mehta, Subba Rao Gangi Setty, Dipshikha Chakravortty
Summary: Intracellular membrane fusion is mediated by SNARE complexes. Salmonella modulates host SNARE machinery to escape lysosomal fusion and maintain its division in the Salmonella-containing vacuole (SCV). Syntaxin 3 (STX3) is critical for bacterial proliferation and fusion of SCV with intracellular vesicles. The interaction of STX3 with SCV is impaired in SPI-2 encoded T3SS mutant. These findings highlight the importance of SPI-2 encoded effectors in the interaction with host SNAREs for Salmonella pathogenesis.
Article
Biochemistry & Molecular Biology
Xiaohui Qiu, Ying Li, Yingli Wang, Xinyu Gong, Yaru Wang, Lifeng Pan
Summary: The crystal structures of the active Arl8b bound to the RUN domains of PLEKHM1 and SKIP were determined, revealing the molecular mechanisms of their interactions. It was found that there is a competitive relationship between the RUN domains of PLEKHM1 and SKIP and a unique binding mode adopted by these domains.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jialan Lyu, Yuchen Chen, Weiwei Yang, Ting Guo, Xiao Xu, Yongmei Xi, Xiaohang Yang, Wanzhong Ge
Summary: The microRNA miR-210 plays a crucial role in regulating lipid metabolism and preventing neurodegeneration in the Drosophila retina. Its specific expression in photoreceptor neurons affects the functioning of neurons by targeting ACS transcription.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Sport Sciences
Miguel A. Lopez-Gajardo, Desmond McEwan, Juan J. Pulido, Jesus Diaz-Garcia, Francisco M. Leo
Summary: A team's ability to respond positively to adversities, problems, and obstacles is crucial for success in collective sports. This study examined the relationship between resilience, vulnerability under pressure, and individual and team performance. The results showed that resilience was positively associated with subjective individual and team performance, while vulnerability under pressure was negatively related to team performance. These findings highlight the importance of developing strategies to improve team resilience for achieving positive outcomes.
SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS
(2023)
Article
Nutrition & Dietetics
Yentl Huybrechts, Pieter Evenepoel, Mathias Haarhaus, Etienne Cavalier, Geert Dams, Wim Van Hul, Patrick C. C. D'Haese, Anja Verhulst
Summary: This study investigates the association between renal osteodystrophy and bone turnover as well as the expression of osteocytic sclerostin. It also examines the effect of parathyroid hormone on sclerostin expression. The results show that sclerostin is regulated by parathyroid hormone, which in turn affects bone turnover and mineralization. Additionally, the study highlights the functional differences between trabecular and cortical bone, as the expression of osteocytic sclerostin depends on the respective bone compartment. Furthermore, circulating sclerostin has limited diagnostic performance, while changes in skeletal sclerostin expression occur more rapidly and prominently.
Article
Hospitality, Leisure, Sport & Tourism
Miguel A. A. Lopez-Gajardo, Francisco M. M. Leo, Patricia C. C. Jackman, Desmond McEwan
Summary: This multistudy article examines the relationships between variables in an input-mediator-outcome (IMO) framework of team effectiveness in sport over a season. The findings show the significant and reciprocal relationships between teamwork execution and characteristics of resilience, as well as the associations between athlete leadership quality and these mediators and team performance.
SPORT EXERCISE AND PERFORMANCE PSYCHOLOGY
(2023)
Article
Chemistry, Multidisciplinary
David A. Perez A. Berrocal, Thimmalapura M. Vishwanatha, Daniel Horn-Ghetko, J. Josephine Botsch, Laura A. Hehl, Sebastian Kostrhon, Mohit Misra, Ivan Dikic, Paul P. Geurink, Hans van Dam, Brenda A. Schulman, Monique P. C. Mulder
Summary: Protein post-translational modification with ubiquitin (Ub) is a versatile signal regulating cell biology, and diseases associated with impaired Ub modification present opportunities for novel targeted treatments. The final components of the ubiquitination cascade, E3 ligases, have potential as small molecule targets. UbSRhodol, an autoimmolative Ub-based probe, enables detection of changes in E3 transthiolation activity, making it suitable for high-throughput screening and identification of small molecules modulating E3 activity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Editorial Material
Biochemistry & Molecular Biology
Angus I. Lamond, Ivan Dikic, Andre Nussenzweig, Christoph W. Mueller, Janet M. Thornton, Michael B. Yaffe
Article
Cell Biology
Anshu Bhattacharya, Rukmini Mukherjee, Santosh Kumar Kuncha, Melinda Elaine Brunstein, Rajeshwari Rathore, Stephan Junek, Christian Muench, Ivan Dikic
Summary: Acute lysosomal membrane damage reduces functional lysosomes, but they have a recovery potential independent of lysosomal biogenesis and remain unaffected in cells depleted in TFEB and TFE3. A lysosomal membrane regeneration pathway was discovered that depends on ATG8, LIMP2, TBC1D15, dynamin-2, kinesin-5B, and clathrin. LIMP2 acts as a lysophagy receptor, binding ATG8, which recruits TBC1D15 to damaged lysosomal membranes. TBC1D15 interacts with ATG8 proteins and stabilizes the autophagic lysosomal reformation machinery, promoting lysosomal tubule formation and dynamin-2-dependent scission.
NATURE CELL BIOLOGY
(2023)
Article
Plant Sciences
Daniel Gomez-Perez, Monja Schmid, Vasvi Chaudhry, Yiheng Hu, Ana Velic, Boris Macek, Jonas Ruhe, Ariane Kemen, Eric Kemen
Summary: Biotic and abiotic interactions play an important role in shaping natural microbial communities. We have studied the release of antimicrobial proteins by Albugo candida, a plant parasite, and found that these proteins can shape and protect plant habitats. By analyzing Albugo-infected and uninfected Arabidopsis thaliana samples, we discovered numerous negative correlations between Albugo and other phyllosphere microbes. Through analysis of the apoplastic proteome and machine learning predictors, we identified candidate antimicrobial proteins with selective activity against Gram-positive bacteria isolated from A. thaliana, which are important for community structure stability. This is the first report of protist proteins with antimicrobial activity in the apoplastic environment, suggesting their potential as biocontrol tools for targeted manipulation of the microbiome.
Article
Multidisciplinary Sciences
Michael S. S. Werner, Tobias Loschko, Thomas King, Shelley Reich, Tobias Theska, Mirita Franz-Wachtel, Boris Macek, Ralf J. J. Sommer
Summary: Development can be altered to match phenotypes with the environment, and the genetic mechanisms that direct such alternative phenotypes are beginning to be elucidated. Yet, the rules that govern environmental sensitivity vs. invariant development, and potential epigenetic memory, remain unknown. Here, we show that plasticity of nematode mouth forms is determined by histone 4 lysine 5 and 12 acetylation (H4K5/12ac). Acetylation in early larval stages provides a permissive chromatin state, which is susceptible to induction during the critical window of environmental sensitivity.
NATURE COMMUNICATIONS
(2023)
Correction
Multidisciplinary Sciences
Michael S. Werner, Tobias Loschko, Thomas King, Shelley Reich, Tobias Theska, Mirita Franz-Wachtel, Boris Macek, Ralf J. Sommer
NATURE COMMUNICATIONS
(2023)
Review
Immunology
Joao Mello-Vieira, Tobias Bopp, Ivan Dikic
Summary: Cell-autonomous immunity is the first line of defense for cells to recognize and eliminate invasive pathogens. Ubiquitination plays a crucial role in orchestrating immune signaling networks and stimulating the appropriate immune response. This review focuses on how ubiquitin connects pathogen sensing pathways to cellular responses and discusses therapeutic options for enhancing cell-autonomous immune responses.
CURRENT OPINION IN IMMUNOLOGY
(2023)
Article
Cell Biology
Jake Cross, Joanne Durgan, David G. McEwan, Matthew Tayler, Kevin M. Ryan, Oliver Florey
Summary: Cross et al. demonstrate that non-canonical autophagy activation is responsible for the majority of ATG8 lipidation in response to lysosome damage, rather than lysophagy. They show that ATG8 proteins directly conjugate to lysosomal membranes and interact with the lipid transfer protein ATG2. This study reveals a parallel ATG8 response to lysosome damage that is mechanistically distinct from lysophagy and involves important links to lipid transfer and dynamics.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemical Research Methods
Thomas Barske, Philipp Spaet, Hendrik Schubert, Peter Walke, Boris Macek, Martin Hagemann
Summary: Protein phosphorylation by serine/threonine protein kinases (Spk) is an important mechanism for adjusting cellular processes. This study found that SpkB is a crucial regulator in cyanobacteria, involved in phosphorylating the PII protein and other proteins, with implications for carbon metabolism and protein composition.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Multidisciplinary Sciences
Elias Einig, Chao Jin, Valentina Andrioletti, Boris Macek, Nikita Popov
Summary: Deregulation of RNA Polymerase II (RNAPII) leads to collisions with DNA synthesis machinery, resulting in DNA damage and genomic instability in tumor cells. This study provides evidence that elongating RNAPII can activate the ATM kinase at the conflict regions to stimulate DNA repair. The study also reveals the involvement of WRNIP1 in limiting ATM activation during the normal cell cycle.
NATURE COMMUNICATIONS
(2023)
Article
Endocrinology & Metabolism
Yentl Huybrechts, Raphael De Ridder, Ellen Steenackers, Jean-Pierre Devogelaer, Geert Mortier, Gretl Hendrickx, Wim Van Hul
Summary: Paget's disease of bone (PDB) is a bone disorder caused by pathogenic variants in genes such as Sequestosome 1 (SQSTM1), PFN1, and ZNF687. In this study, coding regions of PFN1 and ZNF687 were screened in a Belgian PDB cohort, and rare non-synonymous coding variants were identified in ZNF687. The study supports the involvement of genetic variation in ZNF687 in classical PDB, expanding its mutational spectrum.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Article
Hospitality, Leisure, Sport & Tourism
Miguel A. Lopez-Gajardo, Francisco M. Leo, Patricia C. Jackman, Desmond McEwan
Summary: The purpose of this multistudy article was to investigate the relationships between variables in the input-mediator-outcome framework of team effectiveness in sports. The findings revealed significant and reciprocal positive relationships between teamwork execution and characteristics of resilience, as well as significant and reciprocal negative relationships between teamwork execution and vulnerability under pressure. The study also found that perceived athlete leadership quality had positive associations with teamwork execution and characteristics of resilience, and negative associations with player-level vulnerability under pressure. Overall, the study highlights the importance of teamwork execution, team resilience, and athlete leadership in determining team performance in sports.
SPORT EXERCISE AND PERFORMANCE PSYCHOLOGY
(2023)