期刊
CELL HOST & MICROBE
卷 18, 期 6, 页码 670-681出版社
CELL PRESS
DOI: 10.1016/j.chom.2015.11.004
关键词
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资金
- Australian Postgraduate Award
- ARC Centre of Excellence in Plant Cell Walls
- Australian NHMRC project grant [GNT1022559]
- ARC/NHMRC Future Fellowship
- NHMRC Career Development Fellowship
- VESKI Fellowship
Transmissible stages of Toxoplasma gondii store energy in the form of the carbohydrate amylopectin. Here, we show that the Ca2+-dependent protein kinase CDPK2 is a critical regulator of amylopectin metabolism. Increased synthesis and loss of degradation of amylopectin in CDPK2 deficient parasites results in the hyperaccumulation of this sugar polymer. A carbohydrate-binding module 20 (CBM20) targets CDPK2 to amylopectin stores, while the EF-hands regulate CDPK2 kinase activity in response to Ca2+ to modulate amylopectin levels. We identify enzymes involved in amylopectin turnover whose phosphorylation is dependent on CDPK2 activity. Strikingly, accumulation of massive amylopectin granules in CDPK2-deficient bradyzoite stages leads to gross morphological defects and complete ablation of cyst formation in a mouse model. Together these data show that Ca2+ signaling regulates carbohydrate metabolism in Toxoplasma and that the post-translational control of this pathway is required for normal cyst development.
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