Article
Medicine, Research & Experimental
Karla Rubio, Addi J. Romero-Olmedo, Pouya Sarvari, Guruprasadh Swaminathan, Vikas P. Ranvir, Diana G. Rogel-Ayala, Julio Cordero, Stefan Guenther, Aditi Mehta, Birgit Bassaly, Peter Braubach, Malgorzata Wygrecka, Stefan Gattenloehner, Achim Tresch, Thomas Braun, Gergana Dobreva, Miguel N. Rivera, Indrabahadur Singh, Johannes Graumann, Guillermo Barreto
Summary: A study revealed that non-canonical ITGB2 signaling activates EGFR and RAS/MAPK/ERK signaling in small cell lung cancer (SCLC), providing a potential target for therapy independent of EGFR mutations.
Article
Oncology
Jiani Liu, Xinyu Peng, Yang Yang, Yao Zhang, Meng Han, Xiaohui Shi, Jie Zheng, Tong Li, Jinxia Chen, Weihua Lv, Yunjiang Liu, Yixin Qi, Lei Zhang, Qi Liu
Summary: The study found that hsa_circ_0058514 can serve as an early plasma biomarker for breast cancer diagnosis with better diagnostic value than conventional tumor markers. It is also a predictor for the efficacy of neoadjuvant chemotherapy. Additionally, the expression level of hsa_circ_0058514 is associated with clinicopathological characteristics in breast cancer patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Bianca Cruz Pachane, Ana Carolina Caetano Nunes, Thais Regiani Cataldi, Kelli Cristina Micocci, Bianca Caruso Moreira, Carlos Alberto Labate, Heloisa Sobreiro Selistre-de-Araujo, Wanessa Fernanda Altei
Summary: Hypoxia in triple-negative breast tumors promotes the secretion of extracellular vesicles, which enhance cell invasion. In this study, we investigated the invasive properties triggered by hypoxic small EVs derived from TNBC tumors. The results showed that hypoxic EVs have increased protein abundance and diversity, and are enriched in pro-invasive pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yujin Lee, Jie Ni, Julia Beretov, Valerie C. C. Wasinger, Peter Graham, Yong Li
Summary: Current clinical tools for breast cancer diagnosis are limited, but liquid biopsy has emerged as a minimally invasive strategy for real-time monitoring of tumor biomarkers. Extracellular vesicles (EVs) play a crucial role in tumor development and metastasis. Small EVs (sEVs) are being investigated as a potential source of novel biomarkers for breast cancer using advanced proteomics and sequencing technologies. This review highlights recent advancements in sEV isolation and analysis and summarizes the identified sEV biomarkers for breast cancer diagnosis, prognosis, and chemoresistance.
Article
Multidisciplinary Sciences
Lin Zhang, Jingkun Qu, Yutao Qi, Yimin Duan, Yu-Wen Huang, Zhifen Zhou, Ping Li, Jun Yao, Beibei Huang, Shuxing Zhang, Dihua Yu
Summary: This study reveals a new mechanism of bone metastasis in breast cancer, demonstrating that EZH2 promotes metastasis through regulating TGF beta signaling, providing potential targets for therapeutic intervention.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Judith Buentzel, Henry Gerd Klemp, Ralph Kraetzner, Matthias Schulz, Gry Helene Dihazi, Frank Streit, Annalen Bleckmann, Kerstin Menck, Darius Wlochowitz, Claudia Binder
Summary: The study found significant differences in the metabolome of microvesicles in the blood of breast cancer patients compared to controls, especially in the abundance of certain metabolites. Using targeted and untargeted mass spectrometry, specific metabolites were identified that distinguished between breast cancer and controls, as well as predicted overall survival in breast cancer patients. This suggests that these metabolites could serve as promising biomarkers and potentially play a role in tumor progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Wenwen Chen, Rongkai Cao, Wentao Su, Xu Zhang, Yuhai Xu, Peng Wang, Zhongqiao Gan, Yingying Xie, Hongjing Li, Jianhua Qin
Summary: The study proposes a simple, quick, and label-free method for isolating circulating exosomes from patient serum. Further validation through miRNA sequencing and RT-PCR reveals a potential new biomarker.
Article
Chemistry, Analytical
Reshma Bano, Neha Soleja, Mohd. Mohsin
Summary: ExoSen is an innovative fluorescence resonance energy transfer (FRET) sensor that can determine the concentration of exosomal EGFRs at both in vitro and in vivo levels. It utilizes a sensing element, MIG6, for A549 exosomes, and detects the conformational change induced by MIG6 binding through a variation in the FRET ratio between ECFP and Venus. ExoSen enables rapid, noninvasive detection of A549 exosomes at a concentration of 3.5 x 109 particles/mL.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
V. Vlaeminck-Guillem
Summary: Exosomes play a significant role in cancer research, including their involvement in pathophysiological mechanisms, as biomarkers, and as therapeutic tools. However, their use in routine clinical practice and management of prostate cancer still requires further confirmation and generalization.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Alex P. Shephard, Peter Giles, Mariama Mbengue, Amr Alraies, Lisa K. Spary, Howard Kynaston, Mark J. Gurney, Juan M. Falcon-Perez, Felix Royo, Zsuzsanna Tabi, Dimitris Parthimos, Rachel J. Errington, Aled Clayton, Jason P. Webber
Summary: The study explored the utility of prostate stromal cell-derived vesicles as indicators of an altered tumor environment in prostate cancer patients. By comparing transcripts in different disease states, a set of mRNAs was identified to successfully discriminate clinical courses of prostate cancer, potentially predicting disease outcomes. Machine learning combined with serum EV analysis and PSA showed improved sensitivity and specificity in detecting prostate cancer progression, showcasing the superiority of this liquid biopsy approach compared to needle biopsy.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Oncology
Carlo Morasso, Alessandra Ricciardi, Daisy Sproviero, Marta Truffi, Sara Albasini, Francesca Piccotti, Federico Sottotetti, Ludovica Mollica, Cristina Cereda, Luca Sorrentino, Fabio Corsi
Summary: The study developed a new SiMoA detection method for directly measuring EVs levels in plasma without prior processing. The research found significantly higher levels of EVs in BC patients, which decreased significantly after cancer surgery. There was no correlation between EVs concentration and clinical features of BC.
BREAST CANCER RESEARCH AND TREATMENT
(2022)
Article
Oncology
Harley Robinson, Matthew J. Roberts, Robert A. Gardiner, Michelle M. Hill
Summary: Biofluid-based biomarker tests show promise for precision medicine in prostate cancer clinical practice. Recent studies have evaluated extracellular vesicles (EV) as potential biomarkers for various aspects of prostate cancer, leading to ongoing validation studies. The challenge now is to translate these findings into robust clinical assays using cost-effective methods. Microfluidic devices combining EV extraction and sensitive antibody-based biomarker detection are being explored for point-of-care applications in personalized medicine.
SEMINARS IN CANCER BIOLOGY
(2023)
Article
Oncology
Yang Yang, Yizhe Wang, Xiaofang Che, Kezuo Hou, Jie Wu, Chunlei Zheng, Yang Cheng, Yunpeng Liu, Xuejun Hu, Jingdong Zhang
Summary: The study revealed that the integrin alpha 5/FAK/STAT3/AKT signaling pathway promotes icotinib resistance and malignancy in NSCLC cells. Targeting this pathway may provide a promising strategy to overcome drug resistance.
Article
Oncology
Lin Liu, Masahiro Kawashima, Masahiro Sugimoto, Kazuhiro Sonomura, Fengling Pu, Wei Li, Masashi Takeda, Takayuki Goto, Kosuke Kawaguchi, Taka-Aki Sato, Masakazu Toi
Summary: The study found that the lipid composition of plasma-derived extracellular vesicles can serve as biomarkers for diagnosing breast cancer as well as identifying its progression and subtypes. By analyzing the lipid profiles, a diagnostic model for breast cancer was established, and several lipids were discovered to effectively differentiate the progression and subtypes of breast cancer. Lipid profiling of plasma-derived extracellular vesicles is a non-invasive and promising approach for diagnosing, staging, and subtyping breast cancer.
Review
Biochemistry & Molecular Biology
Mostafa Mostafazadeh, Nasser Samadi, Houman Kahroba, Behzad Baradaran, Sanya Haiaty, Mohammad Nouri
Summary: Exosomes play potential roles in tumor initiation, progression, and chemoresistance by mediating cell-to-cell communication and affecting drug resistance. Studies have shown that the content of exosomes released from tumor cells may be associated with the clinical outcomes of cancer patients.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ja-Hye Park, Jeong-Eun Lee, Pyong-Gon Moon, Moon-Chang Baek
CELL BIOCHEMISTRY AND FUNCTION
(2015)
Article
Oncology
Pyong-Gon Moon, Jeong-Eun Lee, Young-Eun Cho, Soo Jung Lee, Jin Hyang Jung, Yee Soo Chae, Han-Ik Bae, Young-Bum Kim, In-San Kim, Ho Yong Park, Moon-Chang Baek
CLINICAL CANCER RESEARCH
(2016)
Article
Biochemistry & Molecular Biology
Jeong-Eun Lee, Pyong-Gon Moon, In-Kyu Lee, Moon-Chang Baek
Article
Oncology
Pyong-Gon Moon, Jeong-Eun Lee, Young-Eun Cho, Soo Jung Lee, Yee Soo Chae, Jin Hyang Jung, In-San Kim, Ho Yong Park, Moon-Chang Baek
Article
Chemistry, Medicinal
Mi-Na Song, Pyong-Gon Moon, Jeong-Eun Lee, MinKyun Na, Wonku Kang, Yee Soo Chae, Ji-Young Park, Hoyong Park, Moon-Chang Baek
ARCHIVES OF PHARMACAL RESEARCH
(2012)
Article
Pharmacology & Pharmacy
Bong-Hwa Kim, Pyong-Gon Moon, Jeong-Eun Lee, Soyoung Lee, Sook-Kyung Kim, Jong Kwon Lee, Sang-Hyun Kim, Moon-Chang Baek
INTERNATIONAL JOURNAL OF TOXICOLOGY
(2013)
Article
Biochemical Research Methods
Pyong-Gon Moon, Mi Hee Kwack, Jeong-Eun Lee, Young-Eun Cho, Ji-Hwan Park, Daehee Hwang, Moon Kyu Kim, Jung Chul Kim, Young Kwan Sung, Moon-Chang Baek
JOURNAL OF PROTEOMICS
(2013)
Review
Spectroscopy
Pyong-Gon Moon, Sungyong You, Jeong-Eun Lee, Daehee Hwang, Moon-Chang Baek
MASS SPECTROMETRY REVIEWS
(2011)
Article
Biochemical Research Methods
Young-Eun Cho, Thoudam S. K. Singh, Hyun-Chul Lee, Pyong-Gon Moon, Jeong-Eun Lee, Myung-Hoon Lee, Eung-Chil Choi, Yu-Ju Chen, Sang-Hyun Kim, Moon-Chang Baek
MOLECULAR & CELLULAR PROTEOMICS
(2012)
Article
Biochemical Research Methods
Jeong-Eun Lee, Ja-Hye Park, Pyong-Gon Moon, Moon-Chang Baek
Article
Biochemical Research Methods
Young-Eun Cho, Pyong-Gon Moon, Jeong-Eun Lee, Thoudam S. K. Singh, Wonku Kang, Hyun-Chul Lee, Myung-Hoon Lee, Sang-Hyun Kim, Moon-Chang Baek
Article
Agriculture, Dairy & Animal Science
Seung-Woo Jeon, Jay Ronel Conejos, Jungeun Kim, Min-Jeong Kim, Jeong-Eun Lee, Baek-Seok Lee, Jin-Seung Park, Jun-Ok Moon, Jae-Sung Lee, Hong-Gu Lee
JOURNAL OF DAIRY RESEARCH
(2020)
Article
Agriculture, Dairy & Animal Science
Jungeun Kim, Jeong-Eun Lee, Jae-Sung Lee, Jin-Seung Park, Jun-Ok Moon, Hong-Gu Lee
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2020)
Article
Physiology
Yu-Jung Lee, Jeong-Eun Lee, Hyo-Jung Choi, Jung-Suk Lim, Hyun Jun Jung, Moon-Chang Baek, Jorgen Frokiaer, Soren Nielsen, Tae-Hwan Kwon
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2011)
Article
Biochemical Research Methods
Tanushree Halder, Elke Stroeher, Hui Liu, Yinglong Chen, Guijun Yan, Kadambot H. M. Siddique
Summary: Improving the root system of wheat is crucial for enhancing grain yield and climate resilience. This study identified proteins associated with root length and root mass in wheat using label-free quantitative proteomics. The findings revealed potential biomarkers that could be used for improving the root system of wheat.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Yinhua Pan, Quanqing Zou, Wu Yin, Zhen Huang, Yingzhu Zhao, Zongming Mo, Lihui Li, Jianrong Yang
Summary: A prognostic model of BC based on LNM-related genes was established in this study, providing guidance for prognosis evaluation and precise treatment of BC. The genes related to lymph node metastasis in BC are largely unknown and need further exploration. Searching for potential lymph node metastasis-related genes of BC will provide meaningful biomarkers for BC treatment. Our model, based on TCGA-BRCA data, effectively predicted patient outcomes and classified BC patients. The identified feature genes might have a predictive function in immunotherapy. The results of this study provide a new reference for the prognosis and treatment of BC patients with lymph node metastasis.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Qiushi Liang, Joon M. Pijnenburg, Erikjan Rijkers, Arnold G. Vulto, Ans T. van der Ploeg, Niek P. van Til, Eva C. Vlaar, Jeroen A. A. Demmers, W. W. M. Pim Pijnappel
Summary: Pompe disease is a lysosomal storage disorder characterized by glycogen accumulation in skeletal muscle, leading to profound pathology. This study showed that lentiviral gene therapy with LV-IGF2.GAAco can nearly completely correct disease-associated proteomic changes, supporting its future clinical development as a new treatment option for Pompe disease.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Chuang Liu, Wenjing Zhang, Qianli Dong, Haipeng Liu
Summary: This study fills the knowledge gap about the matrix proteins involved in the formation of mineralized exoskeletons in decapod crustaceans. By comparing with well-studied molluscan biomineralization proteins, it is proposed that decapod crustaceans evolved novel proteins while sharing some proteins with molluscs. This study provides insights into the evolution and adaptation of crustaceans and molluscs in forming their exoskeletons.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Rania D. Baka, Josipa Kules, Angelo Beletic, Vladimir Farkas, Dina Resetar Maslov, Blanka Beer Ljubic, Ivana Rubic, Vladimir Mrljak, Mark McLaughlin, David Eckersall, Zoe Polizopoulou
Summary: This study analyzed serum samples from dogs with epilepsy and identified potential markers of epilepsy, proteins involved in nerve tissue regeneration, and proteins altered by antiepileptic medication. The findings provide insight into the pathogenesis of epilepsy and the effects of medication on extracellular matrix proteins.
JOURNAL OF PROTEOMICS
(2024)
Article
Biochemical Research Methods
Viviane M. Codognoto, Fabiana F. de Souza, Thais R. Cataldi, Carlos A. Labate, Laiza S. de Camargo, Pedro H. Esteves Trindade, Roberto R. da Rosa Filho, Diego J. B. de Oliveira, Eunice Oba
Summary: This study aimed to compare urine proteomics from non- and pregnant buffaloes to identify potential biomarkers of early pregnancy. A total of 798 proteins were reported in the urine considering all groups, and the differential proteins play essential roles during pregnancy.
JOURNAL OF PROTEOMICS
(2024)