Article
Chemistry, Applied
Song Yi Koo, Keum Taek Hwang, Soonjae Hwang, Ki Young Choi, Yun Ji Park, Jae-Hyeong Choi, To Quyen Truong, Sang Min Kim
Summary: Nanoencapsulation significantly improves the bioavailability of fucoxanthin in microalgae. Both types of nanoparticles show controlled release of fucoxanthin and enhance its permeability and absorption in cells and plasma.
Article
Multidisciplinary Sciences
Yu Bai, Tianjun Cao, Oliver Dautermann, Paul Buschbeck, Michael B. Cantrell, Yinjuan Chen, Christopher D. Lein, Xiaohuo Shi, Maxwell A. Ware, Fenghua Yang, Huan Zhang, Lihan Zhang, Graham Peers, Xiaobo Li, Martin Lohr
Summary: Fucoxanthin is a major light-harvesting pigment in ecologically important algae. The biosynthetic pathway of fucoxanthin has been identified by studying knockout mutants of the diatom Phaeodactylum tricornutum. The pathway is more complex than expected and involves the metabolism of diadinoxanthin as a central regulatory hub. The study also reveals the evolution of genes for xanthophyll cycle enzymes and the alternative pathway in brown algae.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agricultural Engineering
Zhi-Peng Wang, Pei-Kang Wang, Yan Ma, Jia-Xue Lin, Cheng-Long Wang, Yu-Xiang Zhao, Xin-Yue Zhang, Bei-Chen Huang, Shou-Geng Zhao, Lei Gao, Jing Jiang, Hai-Ying Wang, Wei Chen
Summary: The study demonstrated that the addition of Laminaria japonica hydrolysate (LPH) is a feasible and efficient strategy to promote fucoxanthin accumulation. In the LPH group, both fucoxanthin content and biomass were significantly increased.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ting-Bin Hao, Yang Lu, Zhong-Hong Zhang, Si-Fen Liu, Xiang Wang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: This study successfully improved carotenoid production in diatom Phaeodactylum tricornutum through genetic modification, particularly by overexpressing key carotenogenic genes involved in the MEP pathway. The elevated expression of carotenogenic genes led to increased total carotenoid content, with significant enhancement in fucoxanthin content in the engineered lines CMK and CMS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xinjie Zhao, Liwei Gao, Xiangzhong Zhao
Summary: In this study, a two-step method was used to purify Fucoxanthin from Phaeodactylum tricornutum, resulting in a purity of 92.03% and a recovery rate of 76.67%. The identification and quantification of Fucoxanthin were determined by HPLC and ESI-MS. The purified Fucoxanthin exhibited strong antioxidant properties in vitro.
Article
Biotechnology & Applied Microbiology
Chaitanya Kumar Reddy Pocha, Wen Yi Chia, Kit Wayne Chew, Heli Siti Halimatul Munawaroh, Pau Loke Show
Summary: This paper discusses the extraction of fucoxanthin, focusing on Phaeodactylum tricornutum and briefly mentioning Chaetoceros calcitrans and Isochrysis galbana. The cultivation, harvesting, and drying processes of these diatoms are briefly discussed, followed by an introduction to traditional and new extraction methods. The limitations of current extraction strategies and the potential for cost-effective technologies are also discussed.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Hugo Pereira, Marta Sa, Ines Maia, Alexandre Rodrigues, Iago Teles, Rene H. Wijffels, Joao Navalho, Maria Barbosa
Summary: This study produced two marine microalgae to evaluate biomass and fucoxanthin production, demonstrating the potential for fucoxanthin production from microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Xiaotong Shen, Kehou Pan, Lin Zhang, Baohua Zhu, Yun Li, Jichang Han
Summary: This study investigated the function of a protein called PtABA4 in the synthesis of fucoxanthin, a valuable carotenoid. The overexpression and knockdown of PtABA4 in Phaeodactylum tricornutum showed significant changes in fucoxanthin accumulation. It is speculated that PtABA4 may form multi-enzyme complexes with other membrane associated enzymes and participate in the formation of various carotenoids.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Chemistry, Medicinal
Yong Fan, Xiao-Ting Ding, Li-Juan Wang, Er-Ying Jiang, Phung Nghi Van, Fu-Li Li
Summary: This study introduces a new method for screening mutants with high fucoxanthin content, successfully identifying a linear correlation between content and fluorescence intensity in the diatom model organism rich in fucoxanthin. By using flow cytometry, high-fucoxanthin-content mutants were sorted out, with a significant increase in fucoxanthin content compared to the wild type after 20 days of cultivation. This efficient, rapid, and high-throughput approach provides a valuable tool for screening fucoxanthin-overproducing mutants.
Article
Multidisciplinary Sciences
A-Hyeon Lee, Hye-Yoon Shin, Jong-Hwi Park, Song Yi Koo, Sang Min Kim, Seung-Hoon Yang
Summary: Pro-inflammatory cytokines are produced and secreted through the activation of NF-kappa B and NLRP3 inflammasome signaling pathways. Fucoxanthin, found in Phaeodactylum tricornutum extracts, has been shown to inhibit the activation of these pathways and decrease the production of major pro-inflammatory cytokines.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Jochen M. Buck, Peter G. Kroth, Bernard Lepetit
Summary: Photosynthetic organisms have evolved various photoprotective mechanisms to prevent high light-induced photodamage, among which energy-dependent fluorescence quenching (qE) serves as a rapid way to dissipate excess absorbed energy. Diatoms, as important primary producers, utilize Lhcx proteins and the xanthophyll cycle pigment diatoxanthin to provide qE. By studying the mechanisms of qE in diatoms, it was found that specific amino acids and peptide motifs play crucial roles in conferring qE, with a tryptophan residue identified as having a major influence on qE establishment. This structural explanation sheds light on the intimate link between Lhcx proteins and diatoxanthin in providing qE in diatoms.
Article
Plant Sciences
Jianquan Song, Hejing Zhao, Linxin Zhang, Zheng Li, Jichang Han, Chengxu Zhou, Jilin Xu, Xiaohui Li, Xiaojun Yan
Summary: This study revealed that the heat shock transcription factor PtHSF1 positively regulates the synthesis of triacylglycerol and fucoxanthin in Phaeodactylum tricornutum. Overexpression of PtHSF1 increased the contents of triacylglycerol and fucoxanthin and upregulated key enzyme genes involved in their biosynthesis pathways. Gene silencing of PtHSF1 had the opposite effect. This study provided insights into the regulation mechanisms of PtHSF1-mediated triacylglycerol and fucoxanthin synthesis.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Agricultural Engineering
Qing He, Hanwen Zhang, Mingyang Ma, Yue He, Jing Jia, Qiang Hu, Yingchun Gong
Summary: This study identifies a heterolobosean amoeba as the most common and destructive predator in P. tricornutum cultures. The addition of 400 mg L-1 NH4HCO3 inhibits amoeba growth without significant impact on algal growth. Additionally, stopping culture mixing at night creates a hypoxia environment that further hinders amoeba growth. Consequently, the periodic supply of NH4HCO3 and the cessation of culture mixing at night can prevent or treat protozoan contamination in mass culture of P. tricornutum.
BIORESOURCE TECHNOLOGY
(2022)
Article
Nutrition & Dietetics
Lena Stiefvatter, Ulrike Neumann, Andreas Rings, Konstantin Frick, Ulrike Schmid-Staiger, Stephan C. Bischoff
Summary: Microalgae such as Phaeodactylum tricornutum can be used as a sustainable and nutritious source, providing benefits such as increased body weight gain and improved composition of gut microbiota and short-chain fatty acids.
Article
Biotechnology & Applied Microbiology
To Quyen Truong, Yun Ji Park, Song Yi Koo, Jae-Hyeong Choi, Altai Enkhbayar, Dae-Geun Song, Sang Min Kim
Summary: In this study, it was found that low light intensity conditions led to higher FX content and productivity. The crucial roles of PtLhcf5 and PtLhcf8 in FCP biosynthesis under low light intensity were revealed. FCP formation only occurred in the early growth stage under light transition, and FCP degradation could be triggered by high light intensity throughout the cultivation period. Therefore, low light intensity conditions in the early growth stage were critical for higher FX productivity.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Stefanie Jaeger, Claudia Buechel
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2019)
Article
Plant Sciences
Kathi Gundermann, Volker Wagner, Maria Mittag, Claudia Buechel
Article
Chemistry, Analytical
Marcus von der Au, Max Schwinn, Katharina Kuhlmeier, Claudia Buechel, Bjoern Meermann
ANALYTICA CHIMICA ACTA
(2019)
Review
Biochemistry & Molecular Biology
Claudia Buechel
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2020)
Article
Chemistry, Physical
Egle Bukarte, Anja Haufe, David Palecek, Claudia Buechel, Donatas Zigmantas
Article
Plant Sciences
Claudio Calvaruso, Anne Rokka, Eva-Mari Aro, Claudia Buechel
Article
Biotechnology & Applied Microbiology
Marcus von der Au, Olga Borovinskaya, Luca Flamigni, Katharina Kuhlmeier, Claudia Buechel, Bjoern Meermann
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2020)
Article
Biochemistry & Molecular Biology
Alessandro Agostini, Claudia Buechel, Marilena Di Valentin, Donatella Carbonera
Summary: In this study, the fucoxanthin chlorophyll-binding proteins (FCPs) from Cyclotella meneghiniana were investigated using optically detected magnetic resonance (ODMR) and time-resolved electron paramagnetic resonance (TR-EPR) to characterize the photoprotective mechanism based on triplet-triplet energy transfer (TTET). The results show that two fucoxanthin molecules exposed to the exterior of the FCP monomers play a photoprotective role, indicating a structural strategy different from related light-harvesting complexes from plants and other microalgae.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Plant Sciences
Julia Krischer, Sarah Koenig, Wolfram Weisheit, Maria Mittag, Claudia Buechel
Summary: The C-terminus of CryP affects the redox state of flavin, while BolA and ID42612 bind to CryP through different mechanisms and are influenced by the redox state of flavin.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Physical
Austeja Mikalciute, Andrius Gelzinis, Mindaugas Macernis, Claudia Buchel, Bruno Robert, Leonas Valkunas, Jevgenij Chmeliov
Summary: Diatoms, a group of marine algae, play a significant role in global oxygen production. By studying and calculating the structure of diatom photosynthetic complexes, the foundation for modeling spectroscopic data can be established.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Claudio Calvaruso, Konstantinos Stefanidis, Claudia Buechel
Summary: This study compared the biochemical properties of photosystem (PS) supercomplexes isolated from diatom cells grown under low light or high light conditions. High light acclimation altered the molecular characteristics of PS and led to changes in a specific group of proteins. These findings provide new insights into the adaptability of diatoms to different environments and the mechanisms of photoprotection.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Plant Sciences
Andrea Fantuzzi, Patrycja Haniewicz, Domenica Farci, M. Cecilia Loi, Keunha Park, Claudia Buechel, Matthias Bochtler, A. William Rutherford, Dario Piano
Summary: PSIIm-S/27 contains PsbS and Psb27 subunits, while PSIIm lacks these subunits. PSIIm-S/27 shows limited oxygen evolution and slow electron transfer compared to PSIIm, but bicarbonate can activate PSIIm-S/27. The binding of PsbS and/or Psb27 inhibits forward electron transfer and lowers bicarbonate binding affinity, and bicarbonate switch plays a protective role in PSII assembly.
Article
Plant Sciences
Rameez Arshad, Claudio Calvaruso, Egbert J. Boekema, Claudia Buechel, Roman Kouril
Summary: The study elucidated the structural organization of PSII and PSI supercomplexes in different diatom species. Diatoms have varying architectures of PSII as an adaptation strategy, while the organization of PSI is conserved among diatoms.
Article
Chemistry, Physical
Andrius Gelzinis, Ramunas Augulis, Claudia Buechel, Bruno Robert, Leonas Valkunas
Summary: Diatoms are a significant group of algae contributing a quarter of the global primary production. Recent structural studies on FCP complexes have provided insights, but there are discrepancies with ultrafast spectroscopy results. Researchers have developed a trimer-based FCP model for Cyclotella meneghiniana based on available structures and electron microscopy data.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Chemistry, Physical
Bernard Lepetit, Claudia Buechel
JOURNAL OF PHYSICAL CHEMISTRY B
(2020)