Article
Plant Sciences
Jianghao Wu, Liwei Rong, Weijun Lin, Lingxi Kong, Dengjie Wei, Lixin Zhang, Jean-David Rochaix, Xiumei Xu
Summary: Photosynthetic organisms perform state transitions in response to changing light conditions to optimize photosynthetic yield and reduce photo-damage. The protein kinase STN7 phosphorylates the light-harvesting complex of photosystem II and interacts with lumen thiol oxidoreductase 1 (LTO1) to maintain its active state through redox interactions between conserved cysteines. Loss of LTO1 affects the kinase activity of STN7 in Arabidopsis.
Article
Biochemistry & Molecular Biology
Benya Nontaleerak, Chatchakorn Eurtivong, Churat Weeraphan, Hansuk Buncherd, Daranee Chokchaichamnankit, Chantragan Srisomsap, Jisnuson Svasti, Rojana Sukchawalit, Skorn Mongkolsuk
Summary: This study found that oxidation of C104 in the NieR protein under NaOCl stress leads to the formation of a disulfide-linked dimer, altering the protein's conformation and thereby abolishing its DNA-binding ability and repressing the expression of target genes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Danielle M. Beaupre, Richard G. Weiss
Summary: The review focuses on the properties and applications of synthetic thiol- and disulfide-based materials, particularly polymers. The emphasis is on soft and self-assembling materials, where interconversion of thiol and disulfide groups can trigger stimulus-responses and self-healing, for both biomedical and non-biomedical applications.
Article
Electrochemistry
Daria A. Burmistrova, Andrey Galustyan, Ivan V. Smolyaninov, Nadezhda T. Berberova
Summary: A range of substituted o-aminophenols have been investigated as redox mediators for thiol oxidation to disulfides. The electrooxidation of o-aminophenols leads to the formation of o-iminobenzoquinones, which react with thiols in solution to form disulfides. The use of 4,6-di-tert-butyl-2-(tert-butylamino)phenol as a redox mediator was found to reduce the overpotential of thiol oxidation, resulting in unsymmetrical disulfides with alkyl, aryl, and heteroaryl substituents.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Natalia Zamorano Cuervo, Nathalie Grandvaux
Summary: Protein disulfide isomerases (PDIs) are enzymes that catalyze redox reactions and have chaperone functions in protein folding. PDIs have catalytic thioredoxin (TRX)-like domains with CXXC motifs for redox reactions, as well as non-catalytic TRX-like domains. Strategies for identifying cysteine oxidative post-translational modifications (Cys ox-PTMs) have been optimized, and analysis of a Cys redoxome dataset suggests the presence of a non-catalytic thiol-disulfide motif in PDIs. Further structural analysis and exploration of other PDIs are needed to understand the role of these motifs.
Article
Biochemistry & Molecular Biology
Rebecca L. Charles, Giancarlo Abis, Beatriz F. Fernandez, Sebastian Guttzeit, Roberto Buccafusca, Maria R. Conte, Philip Eaton
Summary: The study revealed that H2O2 activates sEH by forming an intra-disulfide bond, increasing its catalytic efficiency. This oxidative activation mechanism also sensitizes sEH to inhibition by lipid electrophiles. The renin-angiotensin system regulates the intra-disulfide bond activation of sEH, leading to a decrease in EET/DHET ratio and an increase in blood pressure.
Article
Chemistry, Physical
Xun Zhang, Bin Yang, Wenlong Zhou, Zhenqi Zhu, Yujing Bian, Ruonan Zeng
Summary: This study utilized FT-Raman and THz-TDS spectroscopy techniques to measure the TRS, showing a good correlation between disulfide concentration and characteristic peak intensities, with correlation coefficients of 92.2% and 95.4% for FT-Raman and THz-TDS data, respectively. Solid-state density functional theory analysis revealed that both Raman and terahertz vibrational spectroscopy techniques can quantitatively analyze the ratio of cysteine/cystine, providing a new method for biological analysis of sulfur-containing amino acids and related mixtures in basic science.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Medicine, Research & Experimental
Jie Lei, Qian Zhang, Xuan Jin, Huiru Lu, Shuxiang Wang, Tingting Li, Yanmei Sheng, Fangyan Zhang, Yaxin Zheng
Summary: The drug release mechanism and rate of camptothecin-oleic acid prodrugs linked by disulfide bonds were found to be highly dependent on the reactivity between the disulfide bonds and thiol agents, affecting the release rate of the drug. The extracellular release of CPT was significantly faster than intracellular release, attributed to different release mechanisms.
MOLECULAR PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Peter J. Gollan, Andrea Trotta, Azfar A. Bajwa, Ilaria Mancini, Eva-Mari Aro
Summary: The study identified 60 lumenal proteins in the thylakoid lumen, with the FL fraction primarily involved in PSII assembly and repair, and the MAL proteome enriched in proteins that support the oxygen-evolving complex (OEC). Novel proteins, post-translational modifications, and protein oligomers in the lumen were also discovered.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Moua Yang, Robert Flaumenhaft
Summary: Oxidative stress is a characteristic of many systemic diseases associated with thrombosis, and thiol isomerases play a key role in protein folding. Recent studies show that the oxidoreductase activity of thiol isomerases in thrombus formation is controlled by the redox environment and may potentially control thrombotic potential by modifying substrate networks.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Chemistry, Physical
Qunshou Kong, Zhimin Zhu, Qin Xu, Feng Yu, Qisheng Wang, Zhihua Gu, Kai Xia, Dawei Jiang, Huating Kong
Summary: Drawing inspiration from nature, thylakoid-based nanoarchitectures seamlessly integrate natural components with nanotechnology, offering versatile functionality for biomedical applications. This article provides an overview of thylakoid properties, extraction methods, and recent advancements in the design and application of thylakoid-based photosynthetic nanoarchitectures, along with a discussion of future prospects in the field.
Article
Biochemistry & Molecular Biology
Pramod Subedi, Jason J. Paxman, Geqing Wang, Lilian Hor, Yaoqin Hong, Anthony D. Verderosa, Andrew E. Whitten, Santosh Panjikar, Carlos F. Santos-Martin, Jennifer L. Martin, Makrina Totsika, Begona Heras
Summary: This study identified and characterized a novel Dsb-like protein, BcfH, from Salmonella that has a trimeric structure and unconventional catalytic sites, contributing to fimbrial biogenesis. The structural and functional flexibility in the TRX-fold protein family was demonstrated, with BcfH showing multiple redox functions mimicking eukaryotic PDI activity in bacteria.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Multidisciplinary Sciences
Yunlong Shi, Ari Zeida, Caitlin E. Edwards, Michael L. Mallory, Santiago Sastre, Matias R. Machado, Raymond J. Pickles, Ling Fu, Keke Liu, Jing Yang, Ralph S. Baric, Richard C. Boucher, Rafael Radi, Kate S. Carroll
Summary: This study demonstrates the potential of thiol-based chemical probes in inhibiting coronavirus infections, providing a new strategy for drug development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Yu Ogawa, Megumi Iwano, Toshiharu Shikanai, Wataru Sakamoto
Summary: This study re-evaluated the role of FUZZY ONION LIKE (FZL) protein in regulating thylakoid membrane structure and photosynthesis in plants. The researchers found that FZL is localized on the curved edges of the thylakoid membrane, potentially mediating fusion between grana and stroma lamellae. Mutations in FZL resulted in altered thylakoid morphology and disruptions in photosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemical Research Methods
Xiaolu Li, Austin Gluth, Tong Zhang, Wei-Jun Qian
Summary: Redox post-translational modifications greatly impact protein structure and function, allowing for the regulation of biological processes. Redox proteomics approaches use mass spectrometry to study these modifications and have contributed to our understanding of redox signaling and regulation. Various strategies, including blocking free thiols and using selective reduction and enrichment techniques, are employed to analyze redox PTMs. This review discusses the principles and recent advances in redox proteomics and highlights its applications in generating novel biological insights.
Review
Engineering, Biomedical
Hualin Lan, Yi Wang, Tieyin Yin, Yazhou Wang, Wanqian Liu, Xiaojuan Zhang, Qinsong Yu, Zhaoxu Wang, Guixue Wang
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2016)
Article
Engineering, Biomedical
Shuping Ge, Yi Wang, Jie Tian, Daoxi Lei, Qingsong Yu, Guixue Wang
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2016)
Article
Nanoscience & Nanotechnology
Xue Wu, Yinping Zhao, Chaojun Tang, Tieying Yin, Ruolin Du, Jie Tian, Junli Huang, Hans Gregersen, Guixue Wang
ACS APPLIED MATERIALS & INTERFACES
(2016)
Review
Medical Laboratory Technology
GaoYa, PengJuan, RenZhong, HeNi-ya, LiQing, ZhaoXue-shan, WangMei-mei, WenHong-yan, TangZhi-han, JiangZhi-sheng, WangGui-xue, LiuLu-shan
CLINICA CHIMICA ACTA
(2016)
Article
Plant Sciences
Tingzhang Hu, Shanshan Zhu, Lili Tan, Wenhua Qi, Shuai He, Guixue Wang
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2016)
Article
Environmental Sciences
Da Sun, Chuanren Duan, Yaning Shang, Yunxia Ma, Lili Tan, Jun Zhai, Xu Gao, Jingsong Guo, Guixue Wang
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2016)
Article
Nanoscience & Nanotechnology
Tian Zhou, Qinglei Dong, Yang Shen, Wei Wu, Haide Wu, Xianglin Luo, Xiaoling Liao, Guixue Wang
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2016)
Article
Engineering, Biomedical
Lu Huang, Daoxi Lei, Wenxiu Dong, Chaojun Tang, Guixue Wang
JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING
(2016)
Article
Materials Science, Biomaterials
Da Sun, Dake Xu, Chunguang Yang, Jia Chen, M. Babar Shahzad, Ziqing Sun, Jinlong Zhao, Tingyue Gu, Ke Yang, Guixue Wang
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2016)
Article
Plant Sciences
Guangchao Zang, Hanyan Zou, Yuchan Zhang, Zheng Xiang, Junli Huang, Li Luo, Chunping Wang, Kairong Lei, Xianyong Li, Deming Song, Ahmad Ud Din, Guixue Wang
Article
Engineering, Chemical
Xiuju Yang, Jingjing Shi, Qianying Fu, Shanshan Pu, Zhixin Pan, Chunxiao Lian, Zhiyong Yin, Shengxiong Liu, Guixue Wang
Summary: By simulating small offset collisions and optimizing the parameter matching of seatbelt and airbag, a pre-tensioned force-limiting seatbelt was proposed for maximum driver protection.
Article
Virology
Razi Ullah, Jadoon Khan, Nosheen Basharat, Danqun Huo, Ahmad Ud Din, Guixue Wang
Summary: This study evaluated the association of cardiac biomarkers and CS-induced acute lung damage with disease severity and mortality in COVID-19 patients. The findings showed a correlation between elevated cardiac enzymes, inflammatory biomarkers, and cytokines with COVID-19 infection and mortality. Physicians should pay special attention to cardiac biomarkers in COVID-19 patients.
Correction
Engineering, Biomedical
Jianjun Hu, Juhui Qiu, Yiming Zheng, Tao Zhang, Tieying Yin, Xiang Xie, Guixue Wang
ANNALS OF BIOMEDICAL ENGINEERING
(2016)
Article
Engineering, Biomedical
Jianjun Hu, Juhui Qiu, Yiming Zheng, Tao Zhang, Tieying Yin, Xiang Xie, Guixue Wang
ANNALS OF BIOMEDICAL ENGINEERING
(2016)