Article
Pharmacology & Pharmacy
Qianran Wang, Ruiyuan Cao, Liushuai Li, Jia Liu, Jingjing Yang, Wei Li, Linjie Yan, Yanming Wang, Yunzheng Yan, Jiang Li, Fei Deng, Yiwu Zhou, Manli Wang, Wu Zhong, Zhihong Hu
Summary: This study evaluated the anti-CCHFV efficacy of several nucleoside analogs and identified a novel compound, H44, as a promising drug candidate for the treatment of CCHFV infection in vivo. The findings suggest that T-705, H44, and EIDD-1931 have inhibitory effects on CCHFV infection, with T-705 and H44 providing complete protection in mice models.
ANTIVIRAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Tejabhiram Yadavalli, Joshua Ames, David Wu, Benjamin Ramirez, Navya Bellamkonda, Deepak Shukla
Summary: The nucleoside analogs, including acyclovir, ganciclovir, penciclovir, and famciclovir, all demonstrate excellent loading and release profile. DECON, a carbon material loaded with nucleoside analogs, shows effective treatment of active viral infection in human corneal epithelial cells at very low concentrations. The study also presents a well-tolerated administration of DECON three times daily for four weeks, as evaluated through various techniques.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Chemistry, Medicinal
Guoqiang Yao, Jianchen Yu, Cai Lin, Yujia Zhu, Anna Duan, Mengfeng Li, Jie Yuan, Jiancun Zhang
Summary: Zika virus, a mosquito-borne flavivirus, has caused serious medical consequences and complications in recent years. There are currently no specific treatments or vaccines, highlighting the need for new therapies. In this study, a series of novel nucleoside analogs were synthesized and one compound showed potential as an inhibitor of Zika virus replication. This compound may be a promising candidate for future drug discovery.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Mehwish Jabeen, Melody Dutot, Roxane Fagon, Bernard Verrier, Claire Monge
Summary: Natural marine sulfated polysaccharides with promising antiviral activity could be a safe alternative to synthetic broad-spectrum antiviral drugs.
Article
Virology
Hatice Bolek, Lale Ozisik, Zafer Caliskan, Mine Durusu Tanriover
Summary: This study aimed to investigate the clinical outcomes and cost of illness due to respiratory virus infections in hospitalized adult patients. The results showed that patients infected with influenza had higher rates of clinical support, intensive care unit admission, and mortality, as well as higher average hospitalization costs.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Pharmacology & Pharmacy
Natalie R. Hagen, Marie L. Nguyen, John D. Williams, Terry L. Bowlin, Brian G. Gentry
Summary: The study showed that ADAL-1 inhibitor dCF antagonizes the antiviral effect of MBX-2168 by inhibiting the production of triphosphate, which is the active compound in virus-infected cells. The unique interaction mechanism between MBX-2168 and ADAL-1 and the inhibitory effect of dCF on the compound's activation were identified through the experiments conducted on HSV-1 and HCMV-infected cells.
ANTIVIRAL RESEARCH
(2021)
Article
Infectious Diseases
Yuki Furuse, Raita Tamaki, Akira Suzuki, Taro Kamigaki, Michiko Okamoto, Mariko Saito-Obata, Emiko Nakagawa, Mayuko Saito, Edelwisa Segubre-Mercado, Veronica Tallo, Socorro Lupisan, Hitoshi Oshitani
Summary: This study examined the prevalence of respiratory viruses among children with acute respiratory infections in the Philippines. The research found that the prevalence of respiratory viruses in healthcare facilities was significantly higher than in households, with respiratory syncytial virus and rhinovirus being the most common.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Infectious Diseases
Fen Pan, Bingjie Wang, Hong Zhang, Yingying Shi, Qi Xu
Summary: This study enrolled 90 children, among whom 58 patients (64.4%) tested positive for 13 pathogens using FARP, with 18 patients showing multiple-virus infections. Human rhinovirus/enterovirus were most common in infants and toddlers, while adenovirus were common in the 3-5-year-old group. Only three pathogens were found in the 6-14-year-old group.
BMC INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Hortense Petat, Matthieu Schuers, Christophe Marguet, Xavier Humbert, Francois Le Bas, Andry Rabiaza, Sandrine Corbet, Bryce Leterrier, Astrid Vabret, Meriadeg Ar Gouilh
Summary: This study provides additional information on the relationships between respiratory viruses, which remains limited in primary care.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Immunology
Hangjie Zhang, Aiqing Zhu, George F. Gao, Zhongjie Li
Summary: Respiratory syncytial virus (RSV) is a common virus that causes acute respiratory tract infections in children. A study analyzing data from 2014 to 2018 in China found an overall infection rate of 17.3% among children under 5 years old, with the majority being hospitalized. Infants under 6 months old were at higher risk of infection, especially during the winter season. Cough was the most common symptom of RSV infection, and infants under 6 months old were more likely to develop bronchopneumonia.
Article
Virology
Guohong Zhu, Dan Xu, Yuanyuan Zhang, Tianlin Wang, Lingyan Zhang, Weizhong Gu, Meiping Shen
Summary: The study found that the rates of viral infection in children vary by different ages and seasons, with the highest positivity rate of adenovirus appearing in the preschool period, respiratory syncytial virus being highest in infants, and influenza virus increasing with age. The total positive rate of viral infection in different seasons is highest in winter, with influenza positivity rate also being highest in winter.
Review
Chemistry, Medicinal
Shadma Wahab, Irfan Ahmad, Safia Irfan, Ayesha Siddiqua, Shazia Usmani, Parwez Ahmad
Summary: This comprehensive review focuses on the role of licorice in managing respiratory infections caused by viruses and bacteria, highlighting its antiviral and antimicrobial properties. The pharmacological actions of licorice in modulating the immune system and inhibiting inflammation are discussed. The authors emphasize the need for further long-term research to evaluate the effectiveness of licorice in respiratory infections.
MINI-REVIEWS IN MEDICINAL CHEMISTRY
(2022)
Review
Pharmacology & Pharmacy
Eleonora Anci, Camille Braun, Annalisa Marinosci, Frederique Rodieux, Elise Midun, Maria-Jose Torres, Jean-Christoph Caubet
Summary: Viruses may cause non-allergic drug skin reactions and also play important roles in drug-induced skin reactions, especially in DRESS syndrome. Understanding the mechanisms of virus- and drug-induced skin reactions is crucial for clinical reflection and patient care.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rui Liang, Gang Wang, Ding Zhang, Gang Ye, Mengxia Li, Yuejun Shi, Jiale Shi, Huanchun Chen, Guiqing Peng
Summary: African swine fever, caused by the ASFV virus, lacks effective treatments, but the study identified a potential inhibitor target in the dUTPase enzyme. The crystal structures of ASFV and swine dUTPase revealed structural differences and a novel two-subunit active site in ASFV. Comparative analysis of dUTPase structures in the PDB database highlighted the role of C-terminal beta-strand orientation in determining enzyme type, with implications for future inhibitor development.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Immunology
Meghana Nadiger, Prithvi Sendi, Paul A. Martinez, Balagangadhar R. Totapally
Summary: In hospitalized children in the United States, HMPV infection is less common than RSV infection. Complex chronic conditions are more prevalent in children hospitalized with HMPV infection. Hospitalization with HMPV is associated with longer length of stay and higher hospital charges. The adjusted mortality is similar with both infections.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Chemistry, Medicinal
Riccardo Rigo, Elisabetta Groaz, Claudia Sissi
Summary: In the study of designing small molecules to target specific genomic sites controlling gene expression, G-quadruplexes at oncogene promoters have been considered as ideal targets. However, the lack of correlation between G-quadruplex targeting and protein expression hampers pharmaceutical applications. The wide genomic localization and polymorphic behavior of G-quadruplexes may account for this, calling for more focused drug design strategies.
Article
Chemistry, Medicinal
Shuai Tan, Elisabetta Groaz, Raj Kalkeri, Roger Ptak, Brent E. Korba, Piet Herdewijn
Summary: Minor structural modifications of acyclic nucleoside phosphonates can significantly affect their antiviral properties. This study reveals a shift in the selectivity spectrum of acyclic nucleoside phosphonates towards hepatitis B virus (HBV) by 2-substitution of their acyclic chain. The modified nucleotides exhibited significant anti-HBV activities, with the (S)-enantiomers demonstrating higher potency than the (R)-forms. A phosphonodiamidate prodrug of (S)-EHPMPG showed promising anti-HBV activity with high selectivity.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Annelies Stevaert, Ria Van Berwaer, Cato Mestdagh, Julie Vandeput, Els Vanstreels, Valerie Raeymaekers, Manon Laporte, Lieve Naesens
Summary: The S2' motif in the SARS-CoV-2 spike protein plays a crucial role in spike activation, with TMPRSS2 showing more tolerance to variations than TMPRSS13. This insight will help in estimating the impact of S2' motif changes in new variants.
Article
Immunology
Emiel Vanhulle, Thomas D'huys, Becky Provinciael, Joren Stroobants, Anita Camps, Sam Noppen, Dominique Schols, Els J. M. Van Damme, Piet Maes, Annelies Stevaert, Kurt Vermeire
Summary: The study shows that Urtica dioica agglutinin (UDA) isolated from stinging nettle rhizomes can effectively inhibit the replication of various viruses, including SARS-CoV-2. UDA demonstrates inhibitory effects on the transduction and replication of SARS-CoV-2, as well as antiviral activity against different variants. Mechanistically, UDA interferes with the attachment and entry of the virus into cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Sonia De Castro, Annelies Stevaert, Miguel Maldonado, Adrien Delpal, Julie Vandeput, Benjamin Van Loy, Cecilia Eydoux, Jean-Claude Guillemot, Etienne Decroly, Federico Gago, Bruno Canard, Maria-Jose Camarasa, Sonsoles Velazquez, Lieve Naesens
Summary: There is a need for novel antiviral concepts to control SARS-CoV-2 infection. A class of 1,4,4-trisubstituted piperidines have shown promising anti-coronavirus activity. Several analogues were synthesized and evaluated, and four molecules demonstrated micromolar activity against SARS-CoV-2. These compounds were found to inhibit the main protease (M-pro) of SARS-CoV-2, suggesting they could serve as non-covalent inhibitors for further development.
Article
Biochemistry & Molecular Biology
Hui Yang, Elena Eremeeva, Mikhail Abramov, Maarten Jacquemyn, Elisabetta Groaz, Dirk Daelemans, Piet Herdewijn
Summary: An enzymatic method was developed to generate partially base-modified RNA constructs, as well as fully modified RNA constructs featuring multiple modified bases. The efficiency of the fully modified RNA constructs was enhanced by using different T7 RNA polymerase variants. The study also demonstrated the successful incorporation of modified bases into PCR products and the effectiveness of the modified RNA constructs in CRISPR-Cas9 cleavage assays.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Emiel Vanhulle, Jordi Doijen, Joren Stroobants, Becky Provinciael, Sam Noppen, Dominique Schols, Annelies Stevaert, Kurt Vermeire
Summary: Despite vaccination efforts, the Omicron variant of SARS-CoV-2 is still circulating, indicating the need for broad-spectrum antivirals to combat COVID-19 and future coronavirus outbreaks. This study explores the use of cellular electrical impedance (CEI) to monitor cell-cell fusion mediated by the SARS-CoV-2 spike protein. The CEI assay successfully validated the fusion inhibitors EK1 and UDA, and demonstrated the potential of CEI in quantifying fusion efficiency and evaluating mutant spike proteins.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Maria Grazia Martina, Federica Giammarino, Ilaria Vicenti, Elisabetta Groaz, Jef Rozenski, Matteo Incerti, Filomena Sannio, Jean Denis Docquier, Maurizio Zazzi, Marco Radi
Summary: The increasing number of viral outbreaks in recent years calls for the development of novel broad-spectrum antivirals. Non-natural nucleosides have shown to be successful in treating infectious diseases and new base-modified nucleosides were developed for potential antimicrobial activity against different viruses and bacteria.
Article
Pharmacology & Pharmacy
Evelien Vanderlinden, Arnaud Boonen, Sam Noppen, Geert Schoofs, Maya Imbrechts, Nick Geukens, Robert Snoeck, Annelies Stevaert, Lieve Naesens, Graciela Andrei, Dominique Schols
Summary: PRO-2000, a sulfonated polyanionic compound, has anti-SARS-CoV-2 activity by blocking the entry pathway and inhibiting the binding of the S protein to its receptor. It can potentially inhibit a broad range of SARS-CoV-2 variants.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Rui Wang, Annelies Stevaert, Tien Nguyen Truong, Qifei Li, Besir Krasniqi, Benjamin Van Loy, Arnout Voet, Lieve Naesens, Wim Dehaen
Summary: The study investigates the structure-activity relationship of novel coronavirus inhibitors and identifies a class of 1,2,3-triazolo fused triterpenoid compounds with strong antiviral activity against HCoV-229E.
ARCHIV DER PHARMAZIE
(2023)
Article
Biochemistry & Molecular Biology
Michele Ghezzo, Luca Grigoletto, Riccardo Rigo, Piet Herdewijn, Elisabetta Groaz, Claudia Sissi
Summary: I-motifs are non-canonical DNA structures with potential relevance in gene regulation and biotechnological applications. Studying the influence of sugar on iM folding provides insights for the development of sugar modifications in i-structure characterization and applications.
Article
Chemistry, Medicinal
Yuqing Xu, Elisabetta Groaz, Jerome Rihon, Piet Herdewijn, Eveline Lescrinier
Summary: Molecular dynamics simulations revealed the favorable interactions between xylose nucleosides with a phosphonate moiety and specific residues at the active site of the RNA-dependent RNA-polymerase of Enterovirus 71. Xylosyl nucleoside phosphonates containing different nucleobases were synthesized and tested for antiviral activity, with the adenine-containing analogue showing good activity against measles virus and enterovirus-68 without cytotoxicity.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
J. Kaes, E. Vanhulle, L. Seldeslachts, C. Hooft, H. Beeckmans, X. Jin, P. Kerckhof, J. Van Slambrouck, D. Van Raemelonck, G. Vande Velde, L. Naesens, D. Schols, L. J. Ceulemans, R. Vos, K. Vertneire, B. M. Vanaudenaerde
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
