期刊
CELL BIOLOGY INTERNATIONAL
卷 39, 期 4, 页码 435-445出版社
WILEY
DOI: 10.1002/cbin.10404
关键词
bone marrow; cell surface molecules; cytokine-induced killer cell; mesenchymal stem cell
类别
资金
- National Natural Science Foundation of China [30500469]
- China Scholarship Council [2004844133]
- Guang Zhou Large-Scale Instrument Cooperation and Sharing Network Program [2006034]
- Foundation of the Ministry of Education of China for Returned Scholars [2009036]
- Guang Dong Science Research Program [2010B080701074]
Bone marrow mesenchymal stem cells (MSC) were recently found to be associated with some special immunological characteristics, the immunoregulatory effect of MSC was dose-dependent. Low amount of MSC was associated with mild immunosuppression or even immune activation, while the high amount of that was associated with significant immunosuppressive effect. In this study, by using a transwell system, we explored the effect of MSC on the cell cycle, apoptosis rate and the expression of CD69, an activation marker, on the allogeneic cord blood derived cytokine-induced killer(CIK)/natural killer(NK) cells. The results showed that either by transwell or mixed cell-cell co-culture, the MSC can effect CIK/NK cells on the cell cycle, such as arrested in the G0/G1 phase, diminished the ratio of cells in S, G2/M phase, and increased the apoptosis of them. MSC can also depress the expression of CD69 on these killer cells, as well as increased the ratio of CD4(+)CD25(+)CD127(low) T regulatory (Treg) cells in the CIK/NK cell culture system. We draw conclusions that either by transwell or mixed co-culture, the MSC can suppress activation of allogeneic CB-CIK/NK cells in a dose-dependent manner.
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