Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing

Diabetic wound is one of the common complications in diabetic patients, which exhibits chronic, hard-to-heal characteristics. The healing process of wounds is impaired by several factors, including excessive oxidative stress, blocked angiogenesis, and bacterial infection. The therapeutic effects of traditional microneedle patches remain not satisfactory, due to their difficulty simultaneously targeting multiple targets to treat diabetic wounds. As such, there is an urgent need to develop a multifunctional microneedle (MN) patch for promoting the healing of diabetic wounds. A multifunctional MN patch with antioxidant, proangiogenesis, and antibacterial capacities was fabricated to target the pathogenesis of diabetic wounds. Silk fibroin methacryloyl, which has excellent biocompatibility, stable mechanical properties, and well processability, and is selected as the base material for multifunctional MN patches. Prussian blue nanozymes (PBNs) and vascular endothelial growth factor (VEGF) are encapsulated in tips of MN patches, Polymyxin is encapsulated in base layers of MN patches. Based on synergic properties of these components, multifunctional MN patches exhibit excellent biocompatibility, drug-sustained release, proangiogenesis, antioxidant, and antibacterial properties. The developed multifunctional MN patches accelerate diabetic wound healing, providing a potential therapeutic approach.

Automated summary

Diabetic wounds are common and hard-to-heal complications in diabetic patients. The healing process of these wounds is hindered by oxidative stress, blocked angiogenesis, and bacterial infection. Traditional microneedle patches have limitations in targeting multiple aspects of diabetic wounds. In this study, a multifunctional microneedle patch with antioxidant, proangiogenesis, and antibacterial properties was developed using silk fibroin methacryloyl as the base material. This patch exhibited excellent biocompatibility, sustained drug release, and accelerated the healing of diabetic wounds by addressing multiple factors involved in the pathogenesis of diabetic wounds.