期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 373, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.132702
关键词
Photoelectrochemical biosensor; CdS quantum dots; Catalytic hairpin assembly; miRNA-21
资金
- National Natural Science Foundation of China
- Natural Science Foundation of Chongqing
- [21675131]
- [cstc 2020jcyj-zdxmX0003]
A novel Y-shaped DNA-mediated photoelectrochemical (PEC) sensing platform based on Bi2S3@CdIn2S4 photoanode is proposed for the detection of miRNA-21. The PEC biosensor exhibits superior sensitivity and stability, offering potential application value in the early diagnosis and treatment of miRNA-related diseases.
MicroRNA (miRNA) is an ideal biomarker, and the sensitive monitoring of low-abundance miRNA is of great significance for the treatment of cancer at the onset stage. Herein, a novel Y-shaped DNA-mediated photo -electrochemical (PEC) sensing platform based on the Bi2S3@CdIn2S4 photoanode is proposed for the detection of miRNA-21. First, an n-n type Bi2S3@CdIn2S4 heterojunction with tight interface is formed for the first time through a layer-by-layer assembly strategy due to the matched band structures of Bi2S3 and CdIn2S4. The photocurrent intensity of Bi2S3@CdIn2S4/GCE electrode is 1.7-fold higher than that of Bi2S3/GCE electrode and 2.5-fold higher than that of CdIn2S4/GCE electrode under visible light irradiation. Additionally, miRNA-21-triggered catalytic hairpin assembly facilitates the formation of Y-shaped DNA structure with CdS quantum dots (CdS QDs). As the photosensitizer of Bi2S3@CdIn2S4 heterojunction, CdS QDs accelerate the separation of electron-hole pairs in the sensing system and realizes photocurrent signal enhancement, thus realizing the signal on detection of the miRNA-21. The proposed PEC biosensor exhibits a superior sensitivity of 0.18 fM and the recoveries of human serum range from 98.0% to 104% with a relative standard deviation of 5.8-8.9%. Therefore, the constructed PEC biosensor has potential application value in the early diagnosis and treatment of miRNA-related diseases.
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