期刊
NUCLEIC ACIDS RESEARCH
卷 51, 期 5, 页码 2177-2194出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkad029
关键词
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XCI is a crucial process with remarkable diversity among mammalian species. Through analysis of single-cell RNA-seq data and functional assays, we find that FTX shows functional inconsistency in humans, while JPX acts as a major regulator of XIST expression in both species. However, we reveal that different forms of JPX control the production of XIST at different steps depending on the species. Overall, this study highlights the functional versatility of LRGs and suggests diversified mechanisms of action for functionally conserved orthologous LRGs.
X chromosome inactivation (XCI) is an essential process, yet it initiates with remarkable diversity in various mammalian species. XIST, the main trigger of XCI, is controlled in the mouse by an interplay of lncRNA genes (LRGs), some of which evolved concomitantly to XIST and have orthologues across all placental mammals. Here, we addressed the functional conservation of human orthologues of two such LRGs, FTX and JPX. By combining analysis of single-cell RNA-seq data from early human embryogenesis with various functional assays in matched human and mouse pluripotent stem- or differentiated post-XCI cells, we demonstrate major functional differences for these orthologues between species, independently of primary sequence conservation. While the function of FTX is not conserved in humans, JPX stands as a major regulator of XIST expression in both species. However, we show that different entities of JPX control the production of XIST at various steps depending on the species. Altogether, our study highlights the functional versatility of LRGs across evolution, and reveals that functional conservation of orthologous LRGs may involve diversified mechanisms of action. These findings represent a striking example of how the evolvability of LRGs can provide adaptative flexibility to constrained gene regulatory networks.
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