4.6 Article

Creation and gene expression analysis of a giant embryo rice mutant with high GABA content

期刊

MOLECULAR BREEDING
卷 43, 期 1, 页码 -

出版社

SPRINGER
DOI: 10.1007/s11032-022-01353-1

关键词

Gamma-amino butyric acid (GABA); Giant embryo rice; CRISPR/Cas9; RNA-seq; Grain development

向作者/读者索取更多资源

In this study, three giant embryo mutants of different sizes were obtained using CRISPR/Cas9 knockout technology, and it was found that GABA, protein, crude fat, and various mineral contents of the mutants were significantly increased. RNA-seq and qRT-PCR analysis showed that the expression levels of most genes promoting GABA accumulation were upregulated, while the expression levels of most genes involved in GABA degradation were downregulated in one of the mutants. These findings help reveal the molecular regulatory network of GABA metabolism in giant embryo rice and provide a theoretical basis for studying its development mechanisms, which can contribute to the cultivation of GABA-rich rice varieties and enhance human nutrition and health.
Gamma-amino butyric acid (GABA) is a natural non-protein amino acid involved in stress, signal transmission, carbon and nitrogen balance, and other physiological processes in plants. In the human body, GABA has the effects of lowering blood pressure, anti-aging, and activating the liver and kidneys. However, there are few studies on the molecular regulation mechanism of genes in the metabolic pathways of GABA during grain development of giant embryo rice with high GABA content. In this study, three glant embryo (ge) mutants of different embryo sizes were obtained by CRISPR/Cas9 knockout, and it was found that GABA, protein, crude fat, and various mineral contents of the ge mutants were significantly increased. RNA-seq and qRT-PCR analysis showed that in the GABA shunt and polyamine degradation pathways, the expression levels of most of the genes encoding enzymes promoting GABA accumulation were significantly upregulated in the ge-1 mutant, whereas, the expression levels of most of the genes encoding enzymes involved GABA degradation were significantly downregulated in the ge-1 mutant. This is most likely responsible for the significant increase in GABA content of the ge mutant. These results help reveal the molecular regulatory network of GABA metabolism in giant embryo rice and provide a theoretical basis for the study of its development mechanisms, which is conducive to the rapid cultivation of GABA-rich rice varieties, promoting human nutrition, and ensuring health.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据