Article
Agriculture, Multidisciplinary
Qiang You, Dan Li, Haiyan Ding, Hongping Chen, Yuan Hu, Youping Liu
Summary: Polymethoxyflavones (PMFs) are a subgroup of flavonoids with various health benefits. The number and position of hydroxy and methoxy groups play an important role in modulating the pharmacokinetics and metabolites of PMFs, with hydroxy-containing PMFs generally more cytotoxic than those without hydroxy groups.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Xi Xia, Can Chen, Lin Yang, Yuchang Wang, Anan Duan, Dawei Wang
Summary: In this study, metabolome technology was used to analyze the changes in metabolites between young and mature leaves of D. delavayi. A total of 477 metabolites were identified, with 281 differentially accumulated between the two leaf stages. Unique metabolites with important biological functions were found in both young and mature leaves. The results provide valuable insights into the metabolite composition of D. delavayi leaves and lay the foundation for further development and utilization.
Article
Biochemistry & Molecular Biology
Bo-Yang Hsu, Chen-Te Jen, Baskaran Stephen Inbaraj, Bing-Huei Chen
Summary: This study developed two methods for the analysis of ginsenosides in ginseng root residue and compared the results. The findings showed that both methods were able to effectively separate and detect ginsenosides in ginseng root residue. The study also demonstrated the quantitation limits and precision of these methods, and revealed the content range of nine ginsenosides in ginseng root residue.
Article
Environmental Sciences
Thamires Martins, Claudia Schinke, Sonia C. N. Queiroz, Patricia A. de C. Braga, Fabio S. P. Silva, Itamar S. Melo, Felix G. R. Reyes
Summary: The fungus Acremonium camptosporum associated with the marine sponge Aplysina fulva was found to produce antibacterial secondary metabolites. Analysis of mass spectra data revealed the presence of cytotoxic compounds, indicating a close association between the sponge and the fungus with its compounds.
Article
Biochemistry & Molecular Biology
Tong Li, Ruiguo Wang, Peilong Wang
Summary: Biogenic amines (BAs) are formed from amino acids and can have various structures. In our study, a method using ultra-performance liquid chromatography-tandem mass spectrometry was developed to rapidly determine 14 BAs in fish samples. The method showed good linearity, low limits of detection, and satisfactory recovery. It was successfully applied to analyze the concentration levels of BAs in different fish.
Article
Chemistry, Applied
Shi Shen, Xi Chen, Qin Zhuo, Yan Ma, Jingbo Wang, Liyuan Wang, Zhaolong Gong, Junsheng Huo
Summary: By applying untargeted and targeted metabolite analysis, differences in metabolites and compound contents among kiwifruits from different cultivars were found, which can benefit scientific research and new cultivar breeding.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Thouria Bourhim, Myra O. Villareal, Francois Couderc, Abdellatif Hafidi, Hiroko Isoda, Chemseddoha Gadhi
Summary: The study found that argan leaves extract promotes melanin production in B16 cells, which is mainly attributed to its high flavonoid content.
Article
Genetics & Heredity
Ying Yu, Panli Tan, Zhenchao Zhuang, Zhejiong Wang, Linchao Zhu, Ruyi Qiu, Huaxi Xu
Summary: This study identified significant differences in serum metabolites between PCOS patients and healthy controls, primarily involving lipid metabolism pathways. Certain metabolites showed high diagnostic value and could serve as potential biomarkers for PCOS.
BMC MEDICAL GENOMICS
(2021)
Article
Chemistry, Analytical
Srikanth Reddy Surukonti, Surendra Babu Manabolu Surya, Naresh Kumar Katari, Rajender Reddy Yerla
Summary: Betrixaban Maleate, an oral factor Xa inhibitor drug substance, was subjected to stress testing to determine its stability under various degradation conditions. The drug was found to degrade in acidic and alkaline environments, resulting in the formation of three degradation products. The structures of these products were identified using advanced analytical techniques.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Xiaoling Zang, Jie Zhang, Peng Jiao, Xuyan Xue, Zhihua Lv
Summary: In this study, metabolic profiling of lung cancer tissues using UPLC-HRMS revealed promising results in the detection and subtyping of NSCLC. Discriminant metabolites were identified for distinguishing AC and SCC, and the models were validated with external data.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Yang Yang, Bei Fan, Yuwen Mu, Yang Li, Litao Tong, Lili Wang, Liya Liu, Minmin Li, Peipei Sun, Jing Sun, Fengzhong Wang
Summary: This study analyzed the metabolic profiles of black, blue, and white Highland barley grains and found differences in polyphenol content and metabolism pathways. The results provide insights for the development of functional foods using Highland barley.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Applied
Hyun Hee L. Lee, Sang Keun Ha, Yoonsook Kim, Jinyoung Hur
Summary: A new pre-column derivatization method based on dansylation is proposed to overcome the analytical challenges in identifying and quantifying advanced glycation end products (AGEs). The method allows for the separation of different structural isomers of AGEs with low detection limits and wide linear ranges. The developed method shows potential applicability in various fields of food chemistry and biochemistry.
Article
Biochemical Research Methods
Zhenxing Zheng, Hanwen Hu, Li Zeng, Huan Yang, Tianlong Yang, Di Wang, Chuanyang Zhang, Yun Deng, Mingzhi Zhang, Dale Guo, Fang Deng
Summary: This study aimed to rapidly select characteristic compounds for differentiating Citri Sarcodactylis Fructus (CSF) from different origins. By analyzing 31 batches of CSF samples from various regions using ultra-performance liquid chromatography with high-resolution mass spectrometry, differential metabolites were identified, with 30 ketocarboxylic acids selected as marker compounds for discriminating CSF from different sources.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Biochemistry & Molecular Biology
Maria Zorina, Victor V. Dotsenko, Pavel N. Nesterenko, Azamat Temerdashev, Ekaterina Dmitrieva, Yu-Qi Feng, Sanka N. Atapattu
Summary: In this study, phthalylglycyl chloride was proposed as a derivatization agent for the determination of octopamine, adrenaline, and dopamine in urine for the first time. The derivatization process combined reversed-phase liquid chromatography with positive electrospray ionization-high-resolution mass spectrometry. An LC-HRMS method with quantification limits of 5 ng/mL and detection limits of 1.5 ng/mL for all analytes was developed. The derivatization with phthalylglycyl chloride at pH 6.5 and 30 degrees C resulted in a 95-97% yield of derivatives. The proposed method was successfully applied to the analysis of human urine samples and compared with conventional derivatization procedures using 9-fluorenyl-methoxycarbonyl chloride and dansyl chloride.
Article
Food Science & Technology
Yuting Chen, Yujuan Xu, Jing Wen, Yuanshan Yu, Jijun Wu, Lina Cheng, Wai-San Cheang, Wenwen Liu, Manqin Fu
Summary: This study conducted metabolic analysis to compare the metabolites of Citrus reticulata 'Chachi' peel at different harvesting periods. A total of 168 flavonoid metabolites were identified, and their relative contents showed variations throughout the months.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)