Analyzing the Reaction of Orotidine 5?-Phosphate Decarboxylase as a Way to Examine Some Key Catalytic Proposals

This study analyzes the origin of enzyme catalysis by focusing on the reaction of orotidine 5 '-phosphate decarboxylase (ODCase). This reaction involves an enormous catalytic effect of 23 kcal/mol that has been attributed to reactant state destabilization associated with the use of binding energy through the so-called Circe effect. However, our early studies and subsequent key experiments have shown that the presumed effect of the binding energy (namely, the strain exerted by a bond to a phosphate group) does not contribute to the catalysis. In this study, we perform quantitative empirical valence bond calculations that reproduce the catalytic effect of ODCase and the effect of removing the phosphate side chain. The calculations demonstrate that the effect of the phosphate is due to a change in reorganization energy and should not be described as an induced fit effect. Similarly, we show that the overall catalytic effect is due to electrostatic transition state stabilization, which again reflects the smaller reorganization energy in the enzyme than in water. We also elaborate on the problems with the induced fit proposal, including the fact that it does not serve to tell us what the actual origin of the action of the catalytic effect is. In addition to the above points, we use this paper to discuss misconceptions about the meaning of the preorganization effect, as well as other misunderstandings of what is being done in consistent calculations of enzyme catalysis.

Automated summary

This study analyzes the origin of enzyme catalysis, specifically focusing on the reaction of ODCase. The findings suggest that the catalytic effect of ODCase is due to a change in reorganization energy and electrostatic transition state stabilization, rather than the commonly attributed binding energy effect or induced fit effect. The study also discusses misconceptions regarding the preorganization effect and consistent calculations of enzyme catalysis.