期刊
JOURNAL OF PERIODONTOLOGY
卷 87, 期 5, 页码 583-590出版社
WILEY
DOI: 10.1902/jop.2016.150538
关键词
Collagen; gene expression; guided tissue regeneration; interleukin-11; proteoglycans; transforming growth factor beta1
资金
- ITI grant [852-2012]
- Institute Straumann and Botiss
Background: Enamel matrix derivative (EMD) and collagen membranes (CMs) are simultaneously applied in regenerative periodontal surgery. The aim of this study is to evaluate the ability of two CMs and a collagen matrix to adsorb the activity intrinsic to EMD that provokes transforming growth factor (TGF)-beta signaling in oral fibroblasts. Methods: Three commercially available collagen products were exposed to EMD or recombinant TGF-beta 1, followed by vigorous washing. Oral fibroblasts were either seeded directly onto collagen products or were incubated with the respective supernatant. Expression of TGF-beta target genes interleukin (IL)-11 and proteoglycan 4 (PRG4) was evaluated by real time polymerase chain reaction. Proteomic analysis was used to study the fraction of EMD proteins binding to collagen. Results: EMD or TGF-beta 1 provoked a significant increase of IL-11 and PRG4 expression of oral fibroblasts when seeded onto collagen products and when incubated with the respective supernatant. Gene expression was blocked by the TGF-beta receptor I kinase inhibitor SB431542. Amelogenin bound most abundantly to gelatin-coated culture dishes. However, incubation of palatal fibroblasts with recombinant amelogenin did not alter expression of IL-11 and PRG4. Conclusion: These in vitro findings suggest that collagen products adsorb a TGF-beta receptor I kinase-dependent activity of EMD and make it available for potential target cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据