Antibacterial activity of chitosan-polyethylene oxide nanofibers containing silver nanoparticles against aerobic and anaerobic bacteria

Bacterial wound infection is one of the foremost prevalent nosocomial infections. The overuse of an-tibiotics leads to the growing proliferation of antibiotic-resistant microorganisms, a specialty challeng-ing to establish in anaerobic infections and even more so in mixed anaerobic-aerobic disorders. Conse-quently, alternate munitions are urgently needed to expedite wound healing and bactericidal impacts. Considering this, we determined the properties of antibacterial nanofibers (NFs) generated from electro-spun chitosan/polyethylene oxide nanofibers (CS-PEO NFs) integrated with 1% (w/w) silver nanoparticles (SNPs). SNPs were efficiently produced via chemical reduction synthesis and confirmed by color chang-ing to light brown during sonication. SNPs had no discernible influence on the morphology of CS-PEO NFs. Surface Plasmon resonance (SPR) investigated SNPs' size. This technique analyzed that increasing the sonication time to 30 min results in SNP formation with the proper size (4 nm). Following these proce-dures, the nanofibers' characteristics were assessed using physical and mechanical parameters, viscosity, Field Emission-Scanning Electron Microscopy (FESEM), and Fourier transform-infrared spectroscopy (FT-IR). Transmission electron microscopy (TEM) micrographs demonstrated that SNPs produced in chitosan had adequate dispersion. Moreover, in vitro SNP release and encapsulation efficiency were measured, showing a rapid release of up to 36% in the first two hours, and also the effect of CS-PEO NFs and CS-PEO/SNP NFs on the survival of human dermal fibroblast (HDF) and a normal fibroblast cell line (L929) were investigated by MTT colori-metric assay demonstrated the biocompatibility of cells with CS-PEO/SNP NFs. The nanofibers' antibacte-rial properties were compared to evaluate the antibacterial effects of SNPs in the form of CS-PEO/SNP NFs against aerobic and anaerobic bacteria. Antibacterial effect against Escherichia coli, Pseudomonas aerugi-nosa, and Staphylococcus aureus was measured as aerobic microorganisms. Although a significant activity against Pseudomonas aeruginosa was observed, it was effective lesser against E. coli and Staphylococcus aureus. Pseudomonas aeruginosa also had an antibacterial impact against anaerobic bacteria such as Strep-tococcus mutans, Streptococcus sobrinus, and Streptococcus sanguis. CS-PEO/SNP NFs are smashing candi-dates for wound healing and mixed aerobic-anaerobic infections due to their natural origin and favorable results.(c) 2022 Elsevier B.V. All rights reserved.

Automated summary

Bacterial wound infection is a common nosocomial infection, and antibiotic-resistant microorganisms pose a challenge in anaerobic infections and mixed anaerobic-aerobic disorders. In this study, antibacterial nanofibers composed of electrospun chitosan/polyethylene oxide (CS-PEO) nanofibers integrated with silver nanoparticles (SNPs) were investigated for wound healing and bactericidal effects.