The plastisphere of biodegradable and conventional microplastics from residues exhibit distinct microbial structure, network and function in plastic-mulching farmland

The inhomogeneity of plastisphere and soil may result in different microbial communities, thus potentially affecting soil functions. Biodegradable plastics offer an alternative to conventional plastics, nevertheless, the inadequate end-of-life treatment of biodegradable plastics may release more microplastics. Herein, we collected PE and PBAT/PLA microplastics in plastic-mulching farmland in Hebei, China. The bacterial communities of soil, PE and PBAT/PLA plastisphere were investigated using 16 S high-throughput sequencing. We found that the structure of bacterial communities in PBAT/PLA plastisphere were significantly distinct from PE plastisphere and soil. The alpha diversities in PBAT/PLA plastisphere were significantly lower than PE plastisphere and soil. Statistical analysis of differentially ASVs suggested that PBAT/PLA microplastics act as a filter, enriching taxa with the capability to degrade plastic polymers such as Proteobacteria and Actinobacteria. Compared to PE plastisphere, PBAT/PLA plastisphere has networks of less complexity, lower modularity, and more competitive interactions. Predicted metabolic pathways involved in human diseases, carbohydrate metabolism, amino acid metabolism, and xenobiotic biodegradation and metabolism were promoted in PBAT/PLA plastisphere, along with the facilitation in abundance of genes associated with carbon and nitrogen cycling. Our results highlighted the uniqueness of plastisphere of biodegradable microplastics from conventional microplastics and their po-tential impact on soil functions.

Automated summary

The inhomogeneity of plastisphere and soil can lead to different microbial communities, potentially impacting soil functions. Biodegradable plastics show differences in bacterial communities compared to traditional plastics. PBAT/PLA microplastics act as a filter, enriching taxa capable of degrading plastic polymers. Predicted metabolic pathways related to human diseases, carbohydrate metabolism, amino acid metabolism, and xenobiotic biodegradation and metabolism are promoted in PBAT/PLA plastisphere, along with an increase in genes associated with carbon and nitrogen cycling.