Review
Hematology
Andrew P. Stone, Emma Nikols, Daniela Freire, Kellie R. Machlus
Summary: This review provides a comprehensive overview of platelet-derived extracellular vesicles (PEVs) and highlights their historical link to thrombosis and hemostasis. It also discusses the roles that distinguish PEVs and megakaryocyte-derived extracellular vesicles (MKEVs) from their parent cells, as well as their involvement in disease pathology and their potential as novel therapeutics.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Jorge Luis Velez-Paez, Pedro Legua, Pablo Velez-Paez, Estefania Irigoyen, Henry Andrade, Andrea Jara, Fernanda Lopez, Jorge Perez-Galarza, Lucy Baldeon
Summary: This study aimed to evaluate whether mean platelet volume (MPV) and mean platelet volume-to-platelet count (MPV/P) ratio can predict clinical severity and mortality in patients with sepsis. The results showed that both MPV and MPV/P ratio were associated with clinical severity and mortality, suggesting their potential as tools for guiding treatment.
Article
Hematology
Andrea L. Ambrosio, Hallie P. Febvre, Santiago M. Di Pietro
Summary: Platelet alpha-granules play a crucial role in hemostasis and other physiological processes, and this study uncovers the molecular mechanism of alpha-granule biogenesis involving proteins VPS16B, VPS33B, Syntaxin 12 (Stx12), and CCDC22.
Article
Hematology
Manuella Caux, Rana Mansour, Jean-Marie Xuereb, Gaetan Chicanne, Julien Viaud, Alicia Vauclard, Frederic Boal, Bernard Payrastre, Helene Tronchere, Sonia Severin
Summary: This study demonstrates that PIKfyve and its lipid products PtdIns5P and PtdIns(3,5)P-2 play crucial roles in regulating granule integrity in both megakaryocytes and platelets. Inhibition of PIKfyve leads to impaired granule integrity, affecting platelet aggregation and thrombus formation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Oncology
Jiansong Huang, Xin Huang, Yang Li, Xia Li, Jinghan Wang, Fenglin Li, Xiao Yan, Huanping Wang, Yungui Wang, Xiangjie Lin, Jifang Tu, Daqiang He, Wenle Ye, Min Yang, Jie Jin
Summary: Abivertinib, a third-generation tyrosine kinase inhibitor originally designed for targeting EGFR mutations, has shown promising antitumor activity in non-small-cell lung cancer patients, but also exhibits inhibitory effects on MK differentiation and platelet biogenesis, suggesting its potential as a treatment for thrombocythemia.
FRONTIERS OF MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Ji-Yoon Noh
Summary: Platelets, small anucleate blood cells critical in hemostasis, are produced by megakaryocytes from hematopoietic stem cells. Understanding key transcription factors is crucial for platelet biogenesis, as mutations can lead to disorders such as thrombocytopenia. Metabolic changes and diseases like cancer can also impact platelet production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yosuke Morodomi, Sachiko Kanaji, Brian M. Sullivan, Alessandro Zarpellon, Jennifer N. Orje, Eric Won, Ryan Shapiro, Xiang-Lei Yang, Wolfram Ruf, Paul Schimmel, Zaverio M. Ruggeri, Taisuke Kanaji
Summary: Platelets play a crucial role in hemostasis, thrombosis, inflammation, and innate immunity. A study found that an activated form of tyrosyl-tRNA synthetase (YRSACT) has extratranslational activity that enhances megakaryopoiesis and platelet production. This study discovered that YRSACT can mimic inflammatory stress and induce a unique population of megakaryocytes with stem cell and myeloid markers, relying on Toll-like receptor activation and type I interferon signaling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Shuang Li, Jing Liu, Heng Zhang, Zhepeng Sun, Zhu Ying, Yihan Wu, Jianhai Xu, Qun Liu
Summary: This study identified three GST proteins in Toxoplasma gondii, with GST2 playing a critical role in vesicle trafficking by localizing to the Golgi-endosomal system. Loss of TgGST2 led to incorrect localization and decreased expression of several secretory proteins, resulting in reduced invasion capacity and virulence to mice. The findings suggest that TgGST2 contributes to vesicle transport and plays a essential role in the parasite lytic cycle.
Article
Nanoscience & Nanotechnology
Jiapei Yang, Li Xu, Ling Di, Yue Su, Xinyuan Zhu
Summary: PEG, as the gold standard for stealth polymer materials, is widely used in drug delivery due to its excellent properties. However, the lack of understanding about the fate of PEG at the cellular level has hindered its application in diagnosis and therapy. Research showed that PEG could be internalized into cells in 1 hour, localized to lysosomes, cytosol, ER, and mitochondria. The fate of PEG in cells could be regulated by conjugating different small molecules, which is crucial for the rational design of PEGylation for drug delivery.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Biochemistry & Molecular Biology
Fei Yang, Jia Lai, Junzhu Deng, Jun Du, Xi Du, Xiaoqin Zhang, Yiwei Wang, Qianqian Huang, Qian Xu, Gang Yang, Yanjun Zhang, Xilan Zhou, Xiao Zhang, Yuan Yuan, Chunxiang Zhang, Jianming Wu
Summary: Megakaryocytes (MKs), as functional hematopoietic stem cells, play a crucial role in platelet production and maintaining platelet balance. This article reviews the effects of botanical drugs on megakaryocytic differentiation, aiming to provide evidence for their potential use in treating thrombocytopenia and related diseases in the future. The study obtained information from PubMed, Web of Science, and Google Scholar, covering the period from 1994 to 2022.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Hematology
Serge Mbiandjeu, Alessandra Balduini, Alessandro Malara
Summary: Thrombopoiesis is governed by the response of megakaryocyte cytoskeleton to various stimuli, supporting platelet shedding. New cytoskeleton regulators have emerged as critical components in the dynamic protein network that supports platelet production.
THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Clinical Neurology
Melanie Brugger, Fiona Becker-Dettling, Theresa Brunet, Tim Strom, Thomas Meitinger, Eberhard Lurz, Ingo Borggraefe, Matias Wagner
Summary: CCDC186 is proposed as a new disease gene for autosomal recessive neurodevelopmental disorders, with a phenotype associated with developmental delay, epileptic encephalopathy, and failure to thrive. This study reports a second patient with a CCDC186-associated phenotype, further supporting the role of this gene in neurodevelopmental disorders.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shohei Kikuchi, Akinori Wada, Yusuke Kamihara, Imari Yamamoto, Daiki Kirigaya, Kohei Kunimoto, Ryusuke Horaguchi, Takuma Fujihira, Yoshimi Nabe, Tomoki Minemura, Nam H. Dang, Tsutomu Sato
Summary: A potential association between hematopoietic stem cell status in bone marrow and surrounding bone tissue has been investigated, and a significant negative correlation was found between platelet count and bone mineral density (BMD) in the male group. Megakaryocytes derived from bone marrow mononuclear cells were found to produce RANKL, which is negatively correlated with BMD. This study provides new insights into the understanding and potential treatment of osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Simon Lin, Subramanya Pandruvada, Hong Yu
Summary: Treatment with S1PR2 antagonist or S1PR2 shRNA enhanced osteogenesis in BMSCs via different signaling pathways, including increased vesicle trafficking-associated proteins and activation of Wnt/Ca2+ signaling, BMP/Smad signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Charles J. Cho, Dongkook Park, Jason C. Mills
Summary: This study identifies ELAPOR1 as an evolutionarily conserved, trans-Golgi/late endosome M6PR domain-containing protein that is specific to gastric zymogenic cells. It is required for normal secretory granule maturation in both human cell lines and mouse stomach.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Hematology
Ying Lu, Jin-Song Yan, Li Xia, Kang Qin, Qian-Qian Yin, Hong-Tao Xu, Meng-Qing Gao, Xiao-Ning Qu, Yu-Ting Sun, Guo-Qiang Chen
Article
Cell Biology
Xiaoxin Hao, Hao Gu, Chiqi Chen, Dan Huang, Yuzheng Zhao, Li Xie, Yejun Zou, Hui Sophie Shu, Yaping Zhang, Xiaoxiao He, Xiaoyun Lai, Xiaocui Zhang, Bo O. Zhou, Cheng Cheng Zhang, Guo-Qiang Chen, Zhuo Yu, Yi Yang, Junke Zheng
Article
Oncology
Yong Zhou, Wen-Yang Shi, Wei He, Zhao-Wen Yan, Man-Hua Liu, Jing Chen, Yun-Sheng Yang, Yin-Qi Wang, Guo-Qiang Chen, Ying Huang
EXPERIMENTAL CELL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Xinyi Liu, Shaoyong Lu, Kun Song, Qiancheng Shen, Duan Ni, Qian Li, Xinheng He, Hao Zhang, Qi Wang, Yingyi Chen, Xinyi Li, Jing Wu, Chunquan Sheng, Guoqiang Chen, Yaqin Liu, Xuefeng Lu, Jian Zhang
NUCLEIC ACIDS RESEARCH
(2020)
Article
Hematology
Hao Gu, Chiqi Chen, Xiaoxin Hao, Ni Su, Dan Huang, Yejun Zou, Shu-Hai Lin, Xianjun Chen, Denghao Zheng, Ligen Liu, Zhuo Yu, Li Xie, Yaping Zhang, Xiaoxiao He, Xiaoyun Lai, Xiaocui Zhang, Guo-Qiang Chen, Yuzheng Zhao, Yi Yang, Joseph Loscalzo, Junke Zheng
Article
Multidisciplinary Sciences
Meng-Kai Ge, Na Zhang, Li Xia, Cheng Zhang, Shuang-Shu Dong, Zhan-Ming Li, Yan Ji, Min-Hua Zheng, Jing Sun, Guo-Qiang Chen, Shao-Ming Shen
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Yang Wang, Jing Tian, Chao Huang, Jiao Ma, Gaolei Hu, Yalan Chen, Tianshi Wang, Rong Cai, Yong Zuo, Hongsheng Tan, Qiuju Fan, Baijun Dong, Wei Xue, Jing Yi, Guoqiang Chen, Jun Tu, Jinke Cheng
Article
Cell & Tissue Engineering
Jing Chen, Qiong Zhou, Man-Hua Liu, Yun-Sheng Yang, Yin-Qi Wang, Ying Huang, Guo-Qiang Chen
Letter
Oncology
Man-Hua Liu, Xiao-Cui Zhang, Jing Chen, Yun-Sheng Yang, Yin-Qi Wang, Jun-Ke Zheng, Guo-Qiang Chen, Ying Huang
Article
Multidisciplinary Sciences
Chiqi Chen, Xiaoxin Hao, Xiaoyun Lai, Ligen Liu, Jun Zhu, Hongfang Shao, Dan Huang, Hao Gu, Tinghua Zhang, Zhuo Yu, Li Xie, Xiaocui Zhang, Yi Yang, Jun Xu, Yuzheng Zhao, Zhigang Lu, Junke Zheng
Summary: A recent study has shown that B-ALL cells with low SoNar ratio preferentially use oxidative phosphorylation and are more resistant to treatment, suggesting a potential connection between metabolism and B-ALL cell fates.
Article
Biochemistry & Molecular Biology
Ping He, Cheng Zhang, Yan Ji, Meng-Kai Ge, Yun Yu, Na Zhang, Shuo Yang, Jian-Xiu Yu, Shao-Ming Shen, Guo-Qiang Chen
Summary: SNHG8, a chromatin-localized lncRNA, interacted and phase separated with histone H1 variants. It showed stronger binding ability to H1 proteins than linker DNA, leading to increased chromatin condensation and epithelial cell differentiation.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Zhi Pang, Xinran Dong, Huayun Deng, Chengzhi Wang, Xiaodong Liao, Chunhua Liao, Yahui Liao, Weidong Tian, Jinke Cheng, Guoqiang Chen, Haiying Yi, Lei Huang
Summary: Aberrant overexpression of MUC1 and HER2 is associated with breast cancer, and their concomitant expression leads to worse clinical outcome. In a mouse model, coexpression of Her2 and MUC1-CD promotes mammary tumor development and induces changes in tumor lineage plasticity and downregulation of tricarboxylic acid cycle genes. Furthermore, MUC1 enhances the Her2 signaling pathway by inducing Her2/Egfr dimerization.
Article
Multidisciplinary Sciences
Xue Chen, Yanyan Xu, Qidi Chen, Heng Zhang, Yu Zeng, Yan Geng, Lei Shen, Fubin Li, Lei Chen, Guo-Qiang Chen, Chuanxin Huang, Junling Liu
Summary: PTEN-deficient platelets promote autoimmunity in mouse models through excessive activation of T-FH cells and systemic autoimmune pathology.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Johann S. Bergholz, Qiwei Wang, Qi Wang, Michelle Ramseier, Sanjay Prakadan, Weihua Wang, Rong Fang, Sheheryar Kabraji, Qian Zhou, G. Kenneth Gray, Kayley Abell-Hart, Shaozhen Xie, Xiaocan Guo, Hao Gu, Thanh Von, Tao Jiang, Shuang Tang, Gordon J. Freeman, Hye-Jung Kim, Alex K. Shalek, Thomas M. Roberts, Jean J. Zhao
Summary: In this study, it was found that genetic inactivation of PI3K beta can induce anti-tumor immune responses and inhibit the growth of PTEN-deficient breast cancer. This effect is associated with reduced STAT3 signaling and increased expression of immune stimulatory molecules. Pharmacological inhibition of PI3K beta also elicits anti-tumor immunity and synergizes with immunotherapy to inhibit tumor growth. Therefore, the combination of PI3K beta inhibitors and immunotherapy may be a rational treatment approach for PTEN-deficient breast cancer.
Article
Oncology
Yin-Qi Wang, Yun-Sheng Yang, Jing Chen, Man-Hua Liu, Guo-Qiang Chen, Ying Huang
EXPERIMENTAL CELL RESEARCH
(2020)