4.7 Article

Traditional Chinese herb formulas in diet enhance the non-specific immune responses of yellow catfish (Pelteobagrus fulvidraco) and resistance against Aeromonas hydrophila

期刊

FISH & SHELLFISH IMMUNOLOGY
卷 131, 期 -, 页码 631-636

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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.10.050

关键词

Chinese herbal medicine; Pelteobagrus fulvidraco; Non-specific immunity; Serum enzyme activity; Disease resistance

资金

  1. Engineering Center for the Protection and Utilization of Characteristic Fish in Northern Guizhou
  2. [050]

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Supplementing yellow catfish with a traditional Chinese herbal mixture can enhance fish immunity and disease resistance against Aeromonas hydrophila, as indicated by increased activity of immune parameters in serum and induction of cytokine gene expression in the spleen. The results suggest that the TCHM-supplemented diet has a positive impact on fish health.
The effects of a traditional Chinese herbal mixture (TCHM) composed of Glycyrrhiza uralensis, Astragalus mem-branaceus, Rheum palmatum, Catsia tora and Lonicera japonica on immune response and disease resistance of yellow catfish (Pelteobagrus fulvidraco) were studied. Fish were fed diets containing 0% (control), 1.0%, 3.0% or 5.0% TCHM (w/w) for 28 d. Immune parameters including cytokine genes interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha) and Immunoglobulin M (IgM), acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM), catalase (CAT), superoxide dismutase (SOD) and immunoglobulin M (IgM) were measured during the test period. After 28 d of feeding, fish were infected with Aeromonas hydrophila, and mortality was recorded. The TCHM-supplementation diet stimulated ACP, AKP, LZM, CAT, SOD, and IgM activity in serum and induced IL-1 beta, TNF-alpha, and IgM mRNA expression in the spleen. All TCHM groups showed reduced mortality after A. hydrophila infection compared to the control group. These results suggest that the TCHM-supplemented diet can improve fish immunity and disease resistance against A. hydrophila.

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