Article
Multidisciplinary Sciences
Ryoma Tsuchiya, Aino Kaneshima, Masakazu Kobayashi, Maki Yamazaki, Yoko Takasu, Hideki Sezutsu, Yoshiaki Tanaka, Akira Mizoguchi, Kunihiro Shiomi
Summary: Diapause is a major developmental switch in insects evolved as a survival strategy under unfavorable environmental conditions. In the silkworm Bombyx mori, diapause is transgenerationally induced as a maternal effect, with progeny diapause determined by the mother's embryonic environmental temperature. The molecular mechanisms of diapause induction involve a hierarchical pathway regulated by gamma-aminobutyric acid (GABA)ergic and corazonin signaling systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Entomology
Yang Yu, Tian Li, Meiwei Guo, Rong Xiong, Dongshen Yan, Ping Chen
Summary: This study established BmFAMeT6 overexpression and knockout strains in insects and revealed the direct role of FAMeT in regulating insect JH titers and the relationship between farnesoic acid and JH synthesis. It provides a new perspective for understanding JH synthesis and regulation in silkworms and other insects.
Article
Entomology
Shi-Hong Gu, Chien-Hung Chen, Pei-Ling Lin
Summary: The study found that in the embryonic development of Bombyx mori, the protein levels of PTP1B, PTEN, and SHP2 were higher and the enzymatic activities of tyrosine phosphatases increased, suggesting their association with embryonic development.
JOURNAL OF INSECT PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jianfeng Qiu, Taiming Dai, Cheng Luo, Wenzhao Cui, Kai Liu, Jianglan Li, Yanghu Sima, Shiqing Xu
Summary: We found that knocking out the core clock gene Cry1 significantly delays the developmental time in Bombyx mori. This study focuses on the ecdysone and juvenile hormone signalling pathways of fifth instar larvae with the longest developmental time delay. Our results provide insights into the molecular mechanisms by which the circadian clock regulates animal development by maintaining hormonal homeostasis.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bing Li, Pei Hu, Zhi-Hao Huang, Jia-Yue Yang, Jie Wang, Xiu-Zhi Xie, Yu-Han Wang, Chen-Chen Li, Jia-Ping Xu
Summary: This study investigated the expression patterns of BmMettl3 and BmMettl14 in silkworm development and found that m6A methylation is regulated during diapause termination. The absence of BmMettl3 or BmMettl14 led to an increase in the percentage of cells in the S phase. This research contributes to understanding the role of m6A methylation in insect embryogenesis and gametogenesis, as well as diapause initiation and termination.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biology
Lijuan Chen, Zhongjie Zhang, Kai Chen, Ye Yu, Bo Hu, Hongsheng Song, Xiaojing Liu
Summary: This study investigates the expression pattern of diapause hormone (DH) receptor in silkworm pupae and analyzes gene expression profile in the ovaries after DH injection. The results show that differentially expressed genes related to hormone signaling, cellular detoxification, ribosomal proteins, lipid metabolism, and epigenetic modifications are identified. The study provides valuable insights into gene expression variation during the early phase of DH release in silkworm.
Article
Biochemistry & Molecular Biology
Rui Cai, Gang Tao, Ping Zhao, Qingyou Xia, Huawei He, Yejing Wang
Summary: Juvenile hormone (JH) plays a crucial role in insect development, and the POU factor POU-M2 regulates JH synthesis and transcription of JHAMT gene to control insect development and larval state.
Article
Entomology
Guan-Feng Xu, Cheng-Cheng Gong, Hao Lyu, Hui-Min Deng, Si-Chun Zheng
Summary: This study investigated the gene expression control of Bombyx mori embryonic development stages using RNA sequencing, identifying key genes related to DNA replication, transcription, and various developmental pathways. The findings indicate distinct gene expression patterns in early and late embryos, with a focus on organogenesis. These results enhance our understanding of the molecular mechanisms underlying embryonic development and B. mori regulation.
Article
Biochemistry & Molecular Biology
Li Zhang, Haiyang Xu, Yunshi Zhang, Huan Zhang, Zhan Wang, Pengchao Guo, Ping Zhao
Summary: This study determined the three-dimensional crystal structure of JHAMT3 and its binding properties. The results revealed that JHAMT3 shares a similar structural pattern with functional JHAMT2 and is highly expressed in the silk gland of Bombyx mori larvae. It is suggested that JHAMT3 may function as a binding protein for the regulation of juvenile hormone acid levels.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yan-Hua Chen, Tao Jiang, Ayinuer Yasen, Bing-Yan Fan, Juan Zhu, Mei-Xian Wang, Ping Qian, Xing-Jia Shen
Summary: This study found that N6-adenosine methylation (m(6)A) is involved in the diapause regulation of bivoltine silkworms by changing the expression levels of mevalonate kinase (MK). These findings provide a clearer understanding of the role of environmental signals in the diapause regulation of bivoltine silkworms.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biology
Wen-Zhao Cui, Jian-Feng Qiu, Tai-Ming Dai, Zhuo Chen, Jiang-Lan Li, Kai Liu, Yu-Jun Wang, Yang-Hu Sima, Shi-Qing Xu
Summary: Diapause in insects is a developmental transition regulated by the circadian clock system and endocrine system. This study focused on a mutant silkmoth with the Period gene knocked out, which altered the classic diapause pathway and disrupted the predetermined effects of temperature and photoperiod on diapause determination. The impaired circadian clock system in the mutant resulted in up-regulation of the GABA receptor gene, leading to a cascade effect that ultimately reduced the diapause-inducing effect of diapause hormone.
Article
Biochemistry & Molecular Biology
Hisashi Tobita, Takashi Kiuchi
Summary: Diapause, an important trait for insects' survival, is determined by the photoperiod which controls the timing of diapause induction. In the silkworm, maternal conditions and core clock genes like period play a crucial role in the regulation of photoperiodic diapause. Knocking out clock genes disrupt normal feedback loops and affect the ability of female moths to induce diapause in response to photoperiod.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Entomology
Shan-shan Wang, Lei-lei Wang, Yue-xia Pu, Ji-yin Liu, Mei-xian Wang, Juan Zhu, Zhong-yuan Shen, Xing-jia Shen, Shun-ming Tang
Summary: This study reveals the molecular mechanism underlying the parasitism behavior of the tachinid fly on silkworms, showing an increase in the levels of 20-hydroxyecdysone (20E) and juvenile hormone (JH) in parasitized silkworms. The expression patterns of related genes indicate an upregulation of 20E signaling and biosynthesis genes, as well as a downregulation of ecdysone oxidase (EO), a 20E inactivation enzyme. In addition, JH signaling genes are activated, while JH degradation pathway genes are suppressed, resulting in an increase in JH titer. Silencing certain genes can partially recover the precocious wandering behavior of parasitized silkworms.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Hao Guo, Feng Chen, Mingyi Zhou, Weiqun Lan, Wenchang Zhang, Guanwang Shen, Ping Lin, Qingyou Xia, Ping Zhao, Zhiqing Li
Summary: In this study, the role of BmMETTL4 in silkworm embryonic development was investigated. Mutations in BmMETTL4 resulted in developmental defects and lethality in late silkworm embryos. Analysis showed that BmMETTL4 mutation significantly affected genes involved in molecular structure, chitin binding, and serine hydrolase activity. Additionally, expression of cuticular protein genes and collagens was decreased while collagenases were increased, contributing to abnormal embryo development and decreased hatchability in silkworm.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Feifei Ren, Jiming Yan, Xiong Wang, Yukai Xie, Nan Guo, Luc Swevers, Jingchen Sun
Summary: In this study, RNA-seq analysis of the silkworm midgut after BmCPV1 infection identified 2520 differentially expressed genes (DEGs). Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding, cell, and cellular processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Experimental validation confirmed that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)