期刊
JOURNAL OF NEUROSCIENCE
卷 36, 期 6, 页码 1823-1840出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2579-15.2016
关键词
26RFa; Gpr103; P518; QRFP; sleep; zebrafish
资金
- National Institutes of Health [NS-060996, NS-070911, DA-031367]
- Mallinckrodt Foundation
- Rita Allen Foundation
- Brain and Behavior Research Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1429350] Funding Source: National Science Foundation
The hypothalamus plays an important role in regulating sleep, but few hypothalamic sleep-promoting signaling pathways have been identified. Here we demonstrate a role for the neuropeptide QRFP (also known as P518 and 26RFa) and its receptors in regulating sleep in zebrafish, a diurnal vertebrate. Weshow that QRFP is expressed in similar to 10 hypothalamic neurons in zebrafish larvae, which project to the hypothalamus, hindbrain, and spinal cord, including regions that express the two zebrafish QRFP receptor paralogs. We find that the overexpression of QRFP inhibits locomotor activity during the day, whereas mutation of qrfp or its receptors results in increased locomotor activity and decreased sleep during the day. Despite the restriction of these phenotypes to the day, the circadian clock does not regulate qrfp expression, and entrained circadian rhythms are not required for QRFP-induced rest. Instead, we find that QRFP overexpression decreases locomotor activity largely in a light-specific manner. Our results suggest that QRFP signaling plays an important role in promoting sleep and may underlie some aspects of hypothalamic sleep control.
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