Improving physiological relevance of cell culture: the possibilities, considerations, and future directions of the ex vivo coculture model

In vitro models provide an important platform for the investigation of cellular growth and atrophy to inform, or extend mechanistic insights from, logistically challenging in vivo trials. Although these models allow for the identification of candidate mechanistic pathways, many models involve supraphysiological dosages, nonphysiological conditions, or experimental changes relating to individual proteins or receptors, all of which limit translation to human trials. To overcome these drawbacks, the use of ex vivo human plasma and serum has been used in cellular models to investigate changes in myotube hypertrophy, cellular protein synthesis, anabolic and catabolic markers in response to differing age, disease states, and nutrient status. However, there are currently no concurrent guidelines outlining the optimal methodology for this model. This review discusses the key methodological considerations surrounding the use of ex vivo plasma and serum with a focus in application to skeletal muscle cell lines (i.e., C2C12, L6, and LHCN-M2) and human primary skeletal muscle cells (HSMCs) as a means to investigate molecular signaling in models of atrophy and hypertrophy, alongside future directions.

Automated summary

In vitro models provide a platform for studying cellular growth and atrophy, complementing in vivo trials. However, many models have limitations, such as nonphysiological conditions, supraphysiological dosages, or experimental changes that hinder translation to human trials. To overcome these limitations, ex vivo human plasma and serum have been used in cellular models to study molecular signaling in muscle cells. However, guidelines for optimal methodology are currently lacking.