Article
Physiology
Edwin J. Baldelomar, Jennifer R. Charlton, Kevin M. Bennett
Summary: This study developed MRI tools to visualize and measure single-nephron function in the isolated, perfused rat kidney. The researchers used imaging tracers to identify nephrons and measure the delivery and filtration of the tracers at the glomeruli. They found that there was a wide distribution of glomerular filtration rates between nephrons and that the single-kidney glomerular filtration rate remained constant despite variations in nephron number.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
C. L. Walsh, P. Tafforeau, W. L. Wagner, D. J. Jafree, A. Bellier, C. Werlein, M. P. Kuhnel, E. Boller, S. Walker-Samuel, J. L. Robertus, D. A. Long, J. Jacob, S. Marussi, E. Brown, N. Holroyd, D. D. Jonigk, M. Ackermann, P. D. Lee
Summary: Hierarchical phase-contrast tomography (HiP-CT) allows for multiscale imaging of intact human organs, providing insights into organ structures and cellular details. It offers multi-resolution imaging of organotypic functional units and individual specialized cells within intact human organs.
Article
Physiology
Vincenzo Costanzo, Luciano D'Apolito, Donato Sardella, Anna Iervolino, Gaetano La Manna, Giovambattista Capasso, Sebastian Frische, Francesco Trepiccione
Summary: Renal micropuncture has been the choice for measuring SNGFR in animal models, but it is challenging due to complex preparation. Intravital multiphoton microscopy offers another approach with improved resolution. This study presents an innovative linescan-based method that can accurately measure SNGFR in rats and mice.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lei Deng, Jianwei Chen, Yafeng Li, Yutong Han, Guoqing Fan, Jie Yang, Dongjian Cao, Bolin Lu, Kefu Ning, Shuo Nie, Zoutao Zhang, Dan Shen, Yunfei Zhang, Wenbin Fu, Wei Eric Wang, Ying Wan, Sha Li, Yu-Qi Feng, Qingming Luo, Jing Yuan
Summary: This study proposes a cryo-fluorescence micro-optical sectioning tomography (cryo-fMOST) to image whole organs in three dimensions (3D) with submicron resolution. The results demonstrate that cryo-fMOST is capable of acquiring high-resolution morphological details of various whole organs and may be potentially useful for spatial multi-omics.
Article
Physiology
Meredith B. Cobb, Wenjie Wu, Esinam M. Attipoe, Ashley C. Johnson, Michael R. Garrett
Summary: Investigation into the association between nephron number and hypertension and diabetes on chronic kidney disease (CKD) using the HSRA rat model has shown a limited impact of nephron number on kidney injury, especially in response to diabetic hyperglycemia. This suggests that other factors may play a more significant role in the progression of CKD.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Cell Biology
Eun Young Kim, Stuart E. Dryer
Summary: Canonical transient receptor potential-6 (TRPC6) channels play a role in various glomerular diseases, but their involvement in the proteinuric state is still unclear. In this study, TRPC6 knockout was found to attenuate albumin overload-induced proteinuria in rats, although its protective effect on tubulointerstitial compartments depends on the disease model.
Review
Pediatrics
Jennifer R. Charlton, Edwin J. Baldelomar, Dylan M. Hyatt, Kevin M. Bennett
Summary: Studies have shown significant variability in human nephron number, but the mechanisms and etiology behind this variability remain largely unknown. Advances in imaging technology have allowed for a better understanding of renal structure and physiology and their roles in kidney disease progression. New developments in tools for counting nephrons in vivo have also emerged in the last 6 years.
PEDIATRIC NEPHROLOGY
(2021)
Review
Endocrinology & Metabolism
Shohei Fukunaga, Yuki Fujita
Summary: The prevalence of chronic kidney disease (CKD) is increasing globally, making it crucial to reduce the number of patients with CKD. The environment during the fetal and early childhood stages can impact CKD development. Maternal malnutrition can lead to a decrease in glomerular number in infants, increasing the risk of CKD. Various mechanisms, such as suppressed c-Ret expression and excess glucocorticoid exposure, can explain the low glomerular number resulting from maternal malnutrition. Maintaining an adequate glomerular number at birth is essential to prevent CKD development.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Medicine, General & Internal
Gopala K. Rangan, Nilofar Dorani, Miranda M. Zhang, Lara Abu-Zarour, Ho Ching Lau, Alexandra Munt, Ashley N. Chandra, Sayanthooran Saravanabavan, Anna Rangan, Jennifer Q. J. Zhang, Martin Howell, Annette T. Y. Wong
Summary: This study reviewed case reports and studies from 1946 to 2019 on excess water intake and hyponatraemia. The results showed that water intoxication can lead to significant health risks and mortality, with treatment mainly consisting of supportive care and saline solutions.
Article
Agriculture, Dairy & Animal Science
Peter J. Hansen
Summary: Graduate education is crucial for academic scientists as it carries the responsibility of guiding students towards their career and life goals. Being a good mentor involves developing an education framework and adapting it to each student's needs based on experience, advice, and individual personality. Most importantly, mentors should be invested in the success and well-being of their students.
JOURNAL OF ANIMAL SCIENCE
(2023)
Review
Urology & Nephrology
Nobuo Tsuboi, Yusuke Okabayashi
Summary: Obesity-related glomerulopathy (ORG) is a kidney disease associated with obesity, characterized by glomerulomegaly and glomerulosclerosis. The condition is believed to be caused by single-nephron glomerular hyperfiltration, leading to issues such as glomerular hyperfiltration, hypertension, and podocyte dysfunction.
SEMINARS IN NEPHROLOGY
(2021)
Article
Urology & Nephrology
Jennifer R. Charlton, Yanzhe Xu, Teresa Wu, Kim A. deRonde, Jillian L. Hughes, Shourik Dutta, Gavin T. Oxley, Aleksandra Cwiek, Helen P. Cathro, Nathan P. Charlton, Mark R. Conaway, Edwin J. Baldelomar, Neda Parvin, Kevin M. Bennett
Summary: The study utilized CFE-MRI to detect kidney pathology in the transition from AKI to CKD, finding that MRI metrics were as accurate as histological metrics in distinguishing AKI and CKD groups from controls, demonstrating the accuracy and safety of CFE-MRI for detecting whole kidney pathology and providing a foundation for future longitudinal studies in mouse models of AKI and CKD.
KIDNEY INTERNATIONAL
(2021)
Article
Physiology
Sumit R. Monu, D'Anna L. Potter, Tang-Dong Liao, Keyona Nicole King, Pablo A. Ortiz
Summary: Inactivating mutations in the ALMS1 gene in humans cause Alstrom syndrome, which is characterized by obesity, insulin resistance, and renal dysfunction. This study found that ALMS1 plays a role in the control of glomerular hemodynamics by enhancing tubuloglomerular feedback sensitivity and decreasing glomerular filtration rate. ALMS1 gene deletion also leads to glomerular damage and hypertension.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Medicine, General & Internal
Peter Richmond, Lara Hatchuel, Min Dong, Brenda Ma, Branda Hu, Igor Smolenov, Ping Li, Peng Liang, Htay Htay Han, Joshua Liang, Ralf Clemens
Summary: A study in Australia evaluated the dose-finding and adjuvant justification of the SCB-2019 vaccine against SARS-CoV-2. The vaccine, which contains a stabilised trimeric form of the spike protein (S-Trimer) combined with AS03 or CpG/Alum adjuvants, elicited robust humoral and cellular immune responses in both younger and older adults.
Review
Nursing
Miwa Horiuchi-Hirose, Tomoko Fukuoka, Yuka Saeki
Summary: This study aimed to determine perceptions of registered and student nurses regarding the integration of anatomy and physiology into nursing practice. The literature review revealed that both undergraduate student and registered nurses recognized the importance of anatomy and physiology knowledge to nursing practice. They acknowledged its relevance to understanding patient pathophysiology, treatment selection, patient observation, and patient safety.
Article
Urology & Nephrology
Jeffrey H. William, Alexander Morales, Sylvia E. Rosas
SEMINARS IN DIALYSIS
(2020)
Article
Surgery
Scott C. Fligor, Chun Li, Ryoko Hamaguchi, Jeffrey William, Benjamin C. James
Summary: From 2004 to 2016, the rate of parathyroidectomies for SHPT per 1000 ESRD patients decreased, with black patients undergoing surgery at a higher rate. In-hospital mortality trended lower during this period.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Urology & Nephrology
Jeffrey H. H. William, Stewart H. H. Lecker, Robert A. A. Cohen
Summary: Awareness of being observed improves faculty preparation; discomfort about being observed is outweighed by observer input; variable quality of feedback suggests a need for more faculty development.
Letter
Education, Scientific Disciplines
Andrew Matthews, Jeffrey H. William
ADVANCES IN MEDICAL EDUCATION AND PRACTICE
(2021)