Review
Biochemistry & Molecular Biology
Yehong Huo, Jian Hu, Yuanyuan Yin, Peng Liu, Kaiyong Cai, Wei Ji
Summary: Peptides can self-assemble into hierarchical nanostructures with excellent chemical and physical properties, making them widely applicable in bio-/nanotechnology. This review presents the latest research progress on self-assembling peptide-based nanomaterials and their applications in biomedicine and optoelectronics.
Article
Nanoscience & Nanotechnology
Greta Bergamaschi, Angelo Musico, Roberto Frigerio, Alessandro Strada, Andrea Pizzi, Benedetta Talone, Jacopo Ghezzi, Alfonso Gautieri, Marcella Chiari, Pierangelo Metrangolo, Renzo Vanna, Francesca Baldelli Bombelli, Marina Cretich, Alessandro Gori
Summary: This study presents a composite hydrogel obtained by combining a self-assembling peptide with low-temperature gelling agarose, which demonstrates simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Matteo Hirsch, Alvaro Charlet, Esther Amstad
Summary: Many soft natural tissues possess unique mechanical properties achieved through complex interactions between structure and composition. To address the lack of control in soft synthetic materials, a novel 3D printing approach called double network granular hydrogels (DNGHs) has been introduced. These DNGHs are stiff enough to support high tensile loads and significantly tougher than pure polymeric networks. This ink allows for the printing of strong and tough objects with high shape fidelity, opening up new possibilities for soft robotic applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Davide Marin, Michal Bartkowski, Slavko Kralj, Beatrice Rosetti, Paola D'Andrea, Simone Adorinni, Silvia Marchesan, Silvia Giordani
Summary: Nanocomposite hydrogels combining self-assembling tripeptide and carbon nano-onions were developed through covalent and non-covalent approaches. The tripeptide coated the nano-onions and extended their stability in aqueous dispersions. The materials showed high compatibility and could be loaded with CNOs at the highest level reported for nanocarbons. In vitro experiments demonstrated excellent cytocompatibility.
Article
Chemistry, Multidisciplinary
Alvaro Charlet, Matteo Hirsch, Sanjay Schreiber, Esther Amstad
Summary: Sustainable materials, such as recyclable polymers, are becoming more important due to their environmentally friendly nature. Fast prototyping methods are needed for customized products, and there is a need for soft materials that can meet contradictory requirements.
Review
Biotechnology & Applied Microbiology
Andrea Hernandez, Jeffrey D. D. Hartgerink, Simon Young
Summary: Self-assembling peptides are promising biomaterials in biomedicine and material sciences due to their biocompatibility and controlled release properties. They can form versatile nanostructures in response to molecular interactions and mimic biological functions, offering a combinatorial therapy for cytokines and drugs. However, there are challenges to be addressed for their wide application in the clinic. This article describes the characteristics and current applications of self-assembling peptides in immunomodulatory therapeutics.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Fangli Gang, Chunyang Ma, Chunyan Guo, Rui Shi, Yi Xiao
Summary: Chitosan is a widely studied biopolymer for hydrogel preparation, but its low mechanical strength and uncontrollable swelling limit its use as printing ink. This study presents an integrated rapid-prototyping and strength-increasing CS hydrogels 3D printing strategy and demonstrates its excellent anti-swelling and cytocompatibility properties.
Review
Chemistry, Multidisciplinary
Tong Guan, Jiayang Li, Chunying Chen, Ying Liu
Summary: This review introduces the advantages of self-assembling peptide-based hydrogels in wound treatment and emphasizes their precise control capabilities during different stages of wound healing. Furthermore, recent advancements in multifunctional self-assembling peptide-based hydrogels that can regulate and remodel the wound immune microenvironment are summarized.
Review
Chemistry, Multidisciplinary
Aokai Zhang, Feng Wang, Lian Chen, Xianshuo Wei, Maoquan Xue, Feng Yang, Shaohua Jiang
Summary: This review provides a comprehensive description of stimuli-responsive hydrogels fabricated using light-responsive and extrusion-based 3D printing technologies, discussing actuator mechanisms, applications, as well as recent advancements in enhancing toughness and design tools.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Maya K. Monroe, Han Wang, Caleb F. Anderson, Hongpeng Jia, Charles Flexner, Honggang Cui
Summary: Peptides and peptide-based materials play an important role in the treatment of viral infections as active pharmaceutical ingredients, targeting moieties, excipients, carriers, or structural components in drug delivery systems. Peptides have the ability to bind cell receptors, facilitate membrane penetration, and facilitate intracellular trafficking, making them useful in improving targeting efficiency and treatment efficacy in various antiviral systems. Additionally, the self-assembly of peptides provides a diverse library of constructs and biomaterials for systemic and local delivery of antiviral agents.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Chemistry, Multidisciplinary
Ying Zhang, Yingying Yu, Jie Gao
Summary: Nanomedicines offer distinct clinical advantages over traditional therapeutic and diagnostic agents. Supramolecular nanomedicines made from in-situ self-assembling peptides have emerged as a promising strategy, combining the advantages of nanomedicine and prodrug approaches while addressing their respective limitations.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Petr Rozhin, Slavko Kralj, Brigitte Soula, Silvia Marchesan, Emmanuel Flahaut
Summary: Supramolecular hydrogels formed from simple peptides can be improved by the addition of carbon nanomaterials, although their interaction with the peptide structure needs to be investigated. This study compares the performance of single-walled and double-walled carbon nanotubes as additives for a tripeptide hydrogel, with the latter showing superior results. Various spectroscopic techniques, thermogravimetric analysis, microscopy, and rheology data provide detailed insights into the structure and behavior of these nanocomposite hydrogels.
Article
Chemistry, Multidisciplinary
Francesca Cadamuro, Francesco Nicotra, Laura Russo
Summary: Manufacturing technologies such as 3D printing and bio-printing are valuable tools for developing new advanced constructs resembling the structural and functional properties of human organs and tissues. This review focuses on the morphological and biochemical properties of extracellular matrices (ECM) and describes how 3D printing and bioprinting can be used to generate ECM mimics with desired properties.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Maria Gessica Ciulla, Raffaele Pugliese, Fabrizio Gelain
Summary: This study made significant advancements in the field of chemical cross-linked self-assembling peptides (SAPs), bringing them closer to the goal of stiff peptidic materials suitable for the regeneration of various tissues, such as skin, muscle, and lung.
Article
Chemistry, Multidisciplinary
Nicholas A. Sather, Hiroaki Sai, Ivan R. Sasselli, Kohei Sato, Wei Ji, Christopher Synatschke, Ryan T. Zambrotta, John F. Edelbrock, Ryan R. Kohlmeyer, James O. Hardin, John Daniel Berrigan, Michael F. Durstock, Peter Mirau, Samuel Stupp
Summary: Liquid crystalline hydrogels can be 3D printed from aqueous supramolecular polymer inks, with pH and salt concentration influencing intermolecular interactions and ink viscosity. Enhanced hierarchical interactions in high viscosity inks lead to greater nanoscale alignment, enabling the creation of materials with anisotropic properties and scaffolds that promote cell alignment.
Article
Chemistry, Multidisciplinary
Le Tracy Yu, Jeffrey D. Hartgerink
Summary: Collagen is a protein that plays critical roles in living systems. Researchers are interested in preparing mimics of collagen using a triple helix structure. However, current collagen mimics have limitations such as poor thermal stability and slow folding rates. In this study, the researchers used covalent capture to solve these problems while preserving the native three-dimensional structure critical for biological function. They also developed a strategy to selectively capture specific charge paired residues, which expands the utility of covalent capture approach to stabilize biomimetic triple helices. The researchers demonstrated the effectiveness of this approach using peptides derived from Pulmonary Surfactant protein A (SP-A).
Article
Biochemistry & Molecular Biology
Sarah A. H. Hulgan, Jeffrey D. Hartgerink
Summary: This Perspective highlights recent developments in the use of collagen mimetic peptides (CMPs) for understanding and controlling the triple helical structure. Factors influencing triple helix assembly, such as amino acid propensity, pairwise interactions, and long-range effects, have been studied, leading to a better understanding and predictability for peptides with natural amino acids. In addition, unnatural amino acids and covalent cross-linking have been successfully utilized to stabilize the triple helix, allowing for the design and application of more complex and well-controlled collagen mimetic biomaterials.
Article
Biochemical Research Methods
Brett H. Pogostin, Gabriel Saenz, Carson C. Cole, Erin M. Euliano, Jeffrey D. Hartgerink, Kevin J. McHugh
Summary: This study investigates the impact of oxidation state on the release of mannan from a peptide hydrogel. It is found that oxidized mannan is released more slowly from the hydrogel compared to reduced mannan. The slower release rate is attributed to the formation of dynamic imine bonds between oxidized mannan and the hydrogel. In vivo, oxidized mannan is cleared more slowly from the hydrogel. These findings suggest that the oxidation state of mannan affects its release and clearance from the hydrogel, highlighting a potentially important difference between oxidized and reduced mannan.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Kathryn R. Brink, Maxwell G. Hunt, Andrew M. Mu, Ken Groszman, Ky V. Hoang, Kevin P. Lorch, Brett H. Pogostin, John S. Gunn, Jeffrey J. Tabor
Summary: In this study, a display method was developed to investigate the effects of human antimicrobial peptides (AMPs) on the pathogenesis-regulating TCS PhoPQ of Salmonella Typhimurium. The study found that PhoPQ can sense AMPs with diverse sequences, structures, and biological functions. Machine learning was used to identify peptide sub-domains and biophysical features linked to PhoPQ activation.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tyler P. P. Graf, Sherry Yue Qiu, Dhruv Varshney, Mei-Li Laracuente, Erin M. M. Euliano, Pujita Munnangi, Brett H. H. Pogostin, Tsvetelina Baryakova, Arnav Garyali, Kevin J. J. McHugh
Summary: PULSED is a novel drug delivery system that provides a sequence of doses in a single injection, improving patient adherence and therapeutic efficacy. It utilizes high-resolution 3D printing and soft lithography to form biodegradable polymeric microstructures with an open cavity, which are filled with drug and sealed using contactless heating. The system exhibits versatility and compatibility with various drugs, with the potential to simplify drug formulations at a low cost and scale effectively.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Le Tracy Yu, Maria C. Hancu, Mark A. B. Kreutzberger, Amy Henrickson, Borries Demeler, Edward H. Egelman, Jeffrey D. Hartgerink
Summary: The folding of collagen is a complex process, involving the association of three peptides into a triple helical fold. The bundling of collagen triple helices is poorly understood, and to shed light on this process, the collagenous region of complement component 1q was examined. Synthetic peptides were prepared to study self-assembly, revealing that short peptides can assemble into specific octadecamers with the help of noncollagenous sequences. Cryo-electron microscopy showed that the octadecamers formed a hollow, crown-like structure. This research provides insight into the assembly mechanism of collagen and lays the foundation for designing higher order collagen mimetic peptide assemblies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biotechnology & Applied Microbiology
Tsvetelina H. Baryakova, Brett H. Pogostin, Robert Langer, Kevin J. McHugh
Summary: Improving medication adherence is essential for improving population health. Baryakova and colleagues explore the potential of next-generation drug delivery systems to address common barriers to adherence and discuss the impact of these systems across different diseases.
NATURE REVIEWS DRUG DISCOVERY
(2023)
Article
Biochemistry & Molecular Biology
Joseph W. R. Swain, Claire Y. Yang, Jeffrey D. Hartgerink
Summary: Self-assembled nanomaterials show promise in drug delivery, but currently face challenges that need improvement. This work investigates an orthogonal self-assembling system of nanofibrous MultiDomain Peptide (MDP) hydrogels encapsulating liposomes, demonstrating their chemical compatibility and drug-delivering capabilities.
Article
Biochemistry & Molecular Biology
Carson C. Cole, Le Tracy Yu, Mikita Misiura, Joseph Williams III, Thi H. Bui, Jeffrey D. Hartgerink
Summary: Collagen mimetic peptides are made up of triple helices, and their design and synthesis are challenging. In this study, researchers compared the effects of different charge pairs on the triple helical structure and applied their findings to the design and synthesis of collagen.
Article
Materials Science, Biomaterials
Brett H. Pogostin, Marina H. Yu, Alon R. Azares, Erin M. Euliano, Cheuk Sun Edwin Lai, Gabriel Saenz, Samuel X. Wu, Adam C. Farsheed, Sarah M. Melhorn, Tyler P. Graf, Darren G. Woodside, Jeffrey D. Hartgerink, Kevin J. McHugh
Summary: Multidomain peptides (MDPs) are materials that can form injectable supramolecular hydrogels under physiological conditions. Loading antigen into MDP hydrogels does not affect their rheological properties, and they can act as antigen depots. MDP adjuvanted vaccines enhance humoral immunity but do not elicit a strong cellular immune response.
BIOMATERIALS SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Cheuk Sun Edwin Lai, Viridiana Leyva-Aranda, Victoria H. Kong, Tania L. Lopez-Silva, Adam C. Farsheed, Carlo D. Cristobal, Joseph W. R. Swain, Hyun Kyoung Lee, Jeffrey D. Hartgerink
Summary: This study introduces multidomain peptide hydrogels as conduits for bridging sciatic nerve defects and evaluates their efficacy in functional recovery. The results show that conduits filled with anionic peptides may improve postoperative functional recovery, while conduits filled with cationic peptides exhibit poor outcomes.
ACS APPLIED BIO MATERIALS
(2022)