Review
Engineering, Biomedical
X. B. Chen, A. Fazel Anvari-Yazdi, X. Duan, A. Zimmerling, R. Gharraei, N. K. Sharma, S. Sweilem, L. Ning
Summary: Bioinks combine biomaterials and living cells, and are used in extrusion bioprinting to create 3D structures that resemble human tissue or organs. These printed constructs have various applications in tissue engineering and in vitro tissue modeling. The properties of the bioinks, including rheological, mechanical, and biological properties, as well as the printing process, play a crucial role in successful printing and application. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, emphasizing bioink synthesis, characterization, and their influence on the printing process.
BIOACTIVE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Carmen Radeke, Raphael Pons, Marko Mihajlovic, Jonas R. Knudsen, Sarkhan Butdayev, Paul J. Kempen, Charis-Patricia Segeritz, Thomas L. Andresen, Christian K. Pehmoller, Thomas E. Jensen, Johan U. Lind
Summary: In order to achieve the automated fabrication of complex tissue mimicking constructs through 3D bioprinting, it is necessary to develop bioinks that are not only printable and biocompatible but also have integrated cell-instructive properties. Here, we present a scalable technique for generating nanofiber 3D printing inks with unique tissue-guiding capabilities. By tailoring the size and dispersibility of cellulose fibrils, we are able to create nanofibers that match the size and dimensions of natural collagen fibers, allowing for the orientation of cells and the spatial organization of engineered tissues during printing.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Shangjing Xin, Kaivalya A. Deo, Jing Dai, Navaneeth Krishna Rajeeva Pandian, David Chimene, Robert M. Moebius, Abhishek Jain, Arum Han, Akhilesh K. Gaharwar, Daniel L. Alge
Summary: An in-depth investigation was conducted on the dissipation process of HMPs during printing, revealing that external resistance and internal physicochemical properties play a key role in the printing outcome. Understanding these mechanisms can help improve the printability of HMPs and enhance their use in 3D bioprinting.
Review
Biotechnology & Applied Microbiology
Sam P. Tarassoli, Zita M. Jessop, Thomas Jovic, Karl Hawkins, Iain S. Whitaker
Summary: Bioprinting is increasingly using natural biomaterials and there is no established tissue specific bioinks or bioprinting techniques. Further research and standardization of biomechanical assessment types and timing are needed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Yuzhen Wang, Xingyu Yuan, Bin Yao, Shuoji Zhu, Ping Zhu, Sha Huang
Summary: This review discusses the potential and limitations of extrusion-based bioprinting (EBB) for skin regeneration, and introduces strategies for improving the physical properties of bioinks and reinforcing bioinks in EBB approaches. It highlights the applications and effects of current EBB-based bioinks on wound healing, wound scar formation, vascularization, and the regeneration of skin appendages, along with the challenges and future perspectives.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Mohamadmahdi Samandari, Jacob Quint, Alejandra Rodriguez-delaRosa, Indranil Sinha, Olivier Pourquie, Ali Tamayol
Summary: Skeletal muscle injuries can lead to mobility limitations and loss of independence, with current treatments showing variable effectiveness. Skeletal muscle tissue engineering and bioprinting are considered promising solutions for reconstructing complex muscle structures and improving functional recovery.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Martina M. De Santis, Hani N. Alsafadi, Sinem Tas, Deniz A. Bolukbas, Sujeethkumar Prithiviraj, Iran A. N. Da Silva, Margareta Mittendorfer, Chiharu Ota, John Stegmayr, Fatima Daoud, Melanie Koenigshoff, Karl Sward, Jeffery A. Wood, Manlio Tassieri, Paul E. Bourgine, Sandra Lindstedt, Sofie Mohlin, Darcy E. Wagner
Summary: The study introduces a hybrid bioink composed of alginate and rECM for 3D bioprinting, showing promising printing properties and biological inductive capabilities for generating human tissue.
ADVANCED MATERIALS
(2021)
Article
Polymer Science
Alyaa Idrees Abdulmaged, Chin Fhong Soon, Balkis A. Talip, Siti Adibah Ahmad Zamhuri, Salama A. Mostafa, Wenbin Zhou
Summary: Tissue engineering is an innovative approach for treating diseases and replacing body parts using artificial tissues and organs. Bioinks are essential for the success of tissue engineering applications, as they consist of living cells, biomaterials, and bioactive molecules to fabricate tissue-like structures. This study introduces a new multi-material bioink composed of alginate, gelatin, and cholesteryl ester liquid crystals. The formulated bioinks exhibit high printability and resolution, and the printed structures have high shape fidelity and minimum width. The bioinks also show well-defined and stable structures after polymerization with calcium ions. Physicochemical and viability properties of the bioinks are examined, and they demonstrate good performance in terms of thermal stability, water absorption ability, and support for cell activity.
Article
Chemistry, Multidisciplinary
Chongjian Gao, Lan Tang, Huawei Qu, Mingming Wu, Tian Zhou, Chunyi Wen, Pinpin Wang, Nan Xu, Changshun Ruan
Summary: Introduced a strategy of pre-crosslinking and post-crosslinking improvement for methacrylated alginate (AlgMA) bioinks in extrusion bioprinting through the introduction of a small-molecule polycationic crosslinker, methacrylated e-polylysine (e-PLMA). The e-PLMA significantly reinforced the operability and stability of AlgMA bioinks and improved the viability and vitality of cells in printed constructs. This strategy shows great potential in improving Alg-based extrusion bioprinting and expanding its biomedical applications.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Haonan Wang, Huaqing Yu, Xia Zhou, Jilong Zhang, Hongrui Zhou, Haitong Hao, Lina Ding, Huiying Li, Yanru Gu, Junchi Ma, Jianfeng Qiu, Depeng Ma
Summary: This article reviews the significance and recent progress of ECM-based biomaterials in 3D bioprinting, as well as their applications in tissue regeneration and cancer research. There is great potential for future development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Giuseppe Guagliano, Cristina Volpini, Jacopo Camilletti, Francesca Donnaloja, Francesco Briatico-Vangosa, Livia Visai, Paola Petrini
Summary: Bioprinting is a key technique for fabricating cell-laden constructs with controlled geometry. We implemented an unconventional strategy by optimizing the design of structures to promote high oxygenation, mimicking the characteristics of hepatic tissue. Through computational methods and a combination of a priori and a posteriori analyses, we successfully printed constructs using an internally crosslinked bioink and cultured them for up to 12 days, demonstrating the suitability of Hep3Gel for mid/long-term cultures.
Review
Pharmacology & Pharmacy
Annika C. Dell, Grayson Wagner, Jason Own, John P. Geibel
Summary: 3D bioprinting plays a crucial role in tissue engineering in medicine by providing precise and highly customizable methods for creating biological tissues. The selection of appropriate cell ink formulations is vital for process optimization and customization to suit target tissues. Hydrogel-based cell ink formulations show promise for future development in bioprinting technology.
Article
Engineering, Biomedical
Matthew L. Bedell, Angelica L. Torres, Katie J. Hogan, Ziwen Wang, Bonnie Wang, Anthony J. Melchiorri, K. Jane Grande-Allen, Antonios G. Mikos
Summary: The investigation of novel hydrogel systems in osteochondral tissue engineering is important for understanding the relationship between biomaterials, cells, and other factors. This study explored the use of 3D printing to fabricate hydrogel environments that mimic tissue-specific architectures. The researchers characterized a methacryloylated gelatin composite hydrogel system and investigated the effects of additives on bone and cartilage-like matrix deposition and genetic markers in human mesenchymal stem cells (hMSCs). New assays were developed to study the effects of different bioinks and printing techniques, and viable constructs with encapsulated hMSCs were successfully printed. The results demonstrate the potential of these novel hydrogel formulations for promoting osteochondral phenotypes and highlight the importance of bioink additives in printability.
Review
Biochemistry & Molecular Biology
Zhiyuan Kong, Xiaohong Wang
Summary: Large diameter artery defects can be substituted by unbiodegradable polymers, while small diameter blood vessels and microvessels pose challenges in constructing complex vascular models. Vascularization strategies can be categorized into self-generated capillaries from implantation, pre-constructed vascular channels, and 3D printed cell-laden hydrogels. This review discusses the latest advances in 3D bioprinting of vascularized tissues/organs, including new printing techniques and bioinks for promoting angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
C. Alexander Arevalos, Jonathan M. Berg, Jacqueline M. V. Nguyen, Elizabeth L. Godfrey, Claudia Iriondo, K. Jane Grande-Allen
ANNALS OF BIOMEDICAL ENGINEERING
(2016)
Article
Engineering, Biomedical
Patrick S. Connell, Dragoslava P. Vekilov, Christine M. Diaz, Seulgi E. Kim, K. Jane Grande-Allen
ANNALS OF BIOMEDICAL ENGINEERING
(2018)
Article
Engineering, Environmental
Matthew Bedell, Matthew Brown, Alper Kiziltas, Deborah Mielewski, Shakti Mukerjee, Rick Tabor
Article
Materials Science, Biomaterials
Reid L. Wilson, Jennifer P. Connell, K. Jane Grande-Allen
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2019)
Article
Materials Science, Biomaterials
Yu Seon Kim, Jason L. Guo, Johnny Lam, K. Jane Grande-Allen, Paul S. Engel, Antonios G. Mikos
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2019)
Review
Chemistry, Multidisciplinary
Matthew L. Bedell, Adam M. Navara, Yingying Du, Shengmin Zhang, Antonios G. Mikos
Article
Engineering, Biomedical
Reid L. Wilson, Sarah A. Hewes, Anubama Rajan, Shih-Ching Lin, Carolyn Bomidi, Takanori Iida, Mary K. Estes, Anthony W. Maresso, K. Jane Grande-Allen
Summary: Researchers have developed a simple-to-use perfusion system to study the effects of invasive diarrheal pathogens in the human intestine; they found that infection simulation using this system displays important differences and in some ways better replicates the pathophysiology of in vivo infections; in addition, commensal strains can be added to the system to simulate the effects of the host microbiome on the infectious process.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Mani Diba, Gerry L. Koons, Matthew L. Bedell, Antonios G. Mikos
Summary: This study presents the 3D printing of novel biomaterial constructs with shape memory behavior using photo-reactive gelatin nanoparticles as colloidal building blocks. The UV-induced covalent interparticle crosslinks significantly enhance the mechanical properties of printed constructs and provide remarkable control over swelling, degradation, and biomolecule release behavior. These novel colloidal inks show great potential to serve as bio-inks for 3D printing of biomaterials with shape-morphing features in tissue engineering and regenerative medicine applications.
Article
Multidisciplinary Sciences
Mollie M. Smoak, Katie J. Hogan, K. Jane Grande-Allen, Antonios G. Mikos
Summary: This study assesses the potential of electrospun decellularized skeletal muscle extracellular matrix (dECM) to control mouse myoblast growth and myotube formation, aiming to develop a tissue-engineered therapy for skeletal muscle regeneration. The fabrication of a bioactive dECM-based system with tunable physicochemical properties has applications in skeletal muscle engineering, bringing the field closer to developing a therapy to address unmet clinical needs.
Article
Engineering, Biomedical
Amadeus S. Zhu, Tasneem Mustafa, Jennifer P. Connell, K. Jane Grande-Allen
Summary: Mitral valve disease is a common cardiovascular condition characterized by fibrotic remodeling. In this study, it was found that pro-inflammatory cytokines TNF-alpha and IL-1 beta can downregulate myofibroblast differentiation in mitral VICs via the NF-kappa B signaling pathway, suggesting potential new avenues for pharmaceutical treatments for mitral valve disease.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Mechanical
S. M. Mehta, D. R. De Santos, S. Sridhar, V. C. Aguayo, C. A. Meraz, M. Mikos, K. J. Grande-Allen
Summary: A small mechanical testing device was successfully developed by the research team, capable of conducting mechanical and microstructural characterization on a microscope stage, accurately testing mechanical properties of various soft materials with competitive performance and pricing. This innovation bridges the gap in testing microstructural changes left by existing commercial devices, providing a new research tool for the field of Biomedical Engineering.
EXPERIMENTAL TECHNIQUES
(2022)
Article
Nanoscience & Nanotechnology
Madeleine A. Roseen, Monica M. Fahrenholtz, Jennifer P. Connell, Kathryn Jane Grande-Allen
ACS APPLIED BIO MATERIALS
(2020)
Article
Nanoscience & Nanotechnology
Madeleine A. Roseen, Romi Lee, Allison D. Post, Megan Wancura, Jennifer P. Connell, Elizabeth Cosgriff-Hemandez, K. Jane Grande Allen
ACS APPLIED BIO MATERIALS
(2020)