Article
Multidisciplinary Sciences
Zhongshun Liu, Congwei Jiang, Zhangmengxue Lei, Sihan Dong, Linlin Kuang, Chenxu Huang, Ying Gao, Mu Liu, Hui Xiao, Patrick Legembre, Jae U. Jung, Huaping Liang, Xiaozhen Liang
Summary: Type I interferons (IFNs) are the first line of defense against invading pathogens. This study identified a previously unknown protein, PINLYP, that interacts with TBK1 to induce the production of type I IFN. Loss of PINLYP impaired the activation of IRF3 and the production of IFN induced by various viruses and Toll-like receptor ligands. Mice lacking PINLYP were more susceptible to lethal virus infection, highlighting the importance of PINLYP in the host defense against viral infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Yi-dou Hu, Ke Wu, Yuan-jie Liu, Qian Zhang, Hui Shen, Jin Ji, Dong Fang, Song-yang Xi
Summary: The JUP/AGR2/LYPD3 signaling axis plays a crucial role in the malignant features of melanoma. Targeting this signaling axis may lead to the development of promising drugs.
Article
Chemistry, Multidisciplinary
Mederic Degardin, Jimmy Gaudreault, Romane Oliverio, Benjamin Serafin, Catherine Forest-Nault, Benoit Liberelle, Gregory De Crescenzo
Summary: Functionalization of surfaces with proteins is needed in many biomedical and biosensing applications. The E/K coiled-coil peptide heterodimeric system has been found to be advantageous for this purpose. Various strategies of Kcoil grafting for capturing Ecoil-tagged proteins were compared, and the performances in biosensing and cell culture applications were evaluated. The results showed that both thiol coupling and amine coupling methods can be effective for protein capture, with each having its own advantages and limitations.
Article
Biochemistry & Molecular Biology
Alaeddine Safi, Wouter Smagghe, Amanda Goncalves, Qing Wang, Ke Xu, Ana Ibis Fernandez, Benjamin Cappe, Franck B. Riquet, Evelien Mylle, Dominique Eeckhout, Nancy De Winne, Eveline Van De Slijke, Freya Persyn, Geert Persiau, Daniel Van Damme, Danny Geelen, Geert De Jaeger, Tom Beeckman, Jelle Van Leene, Steffen Vanneste
Summary: In this study, a SYMPL-vector set was used to detect protein-protein interactions (PPIs) and kinase activities in plants based on phase separation. The technology allowed easy detection of inducible, binary, and ternary PPIs among cytoplasmic and nuclear proteins in plant cells. Furthermore, a reporter for SNF1-related kinase 1 activity was developed, enabling visualization of tissue-specific, dynamic SnRK1 activity in transgenic Arabidopsis plants.
Article
Medicine, Research & Experimental
Pranav R. H. Joshi, Alice Bernier, Pablo D. Moco, Joseph Schrag, Parminder S. Chahal, Amine Kamen
Summary: This study presents a scalable and robust anion-exchange chromatography (AEX) method for removing empty AAV capsids and enriching AAV vectors, showing good results and comparability for different serotypes.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Materials Science, Biomaterials
Angeline Pelus, Gaelle Bordes, Sophie Barbe, Younes Bouchiba, Callum Burnard, Juan Cortes, Brice Enjalbert, Jeremy Esque, Alejandro Estana, Regis Faure, Anthony K. Henras, Stephanie Heux, Claude Le Men, Pierre Millard, Sebastien Nouaille, Julien Perochon, Marion Toanen, Gilles Truan, Amandine Verdier, Camille Wagner, Yves Romeo, Cedric Y. Montanier
Summary: The development of protein and microorganism engineering has increased expectations for biotechnology in designing emerging biomaterials. By using a Carbohydrate-Binding Module (CBM) as a central core, a versatile strategy can be employed to bring a large diversity of functions to cellulose surfaces.
BIOMATERIALS SCIENCE
(2021)
Article
Food Science & Technology
Jun Heo, Chang Woo Kwon, Juno Lee, Haena Park, Hyunjong Yu, Pahn-Shick Chang
Summary: A novel lipase was discovered from Korean chestnut, showing high purification fold, specific activity against different triglycerides, and maximal activity at alkaline pH. The enzyme exhibited high affinity for its substrate and could be activated by Mg2+ and Ca2+. Its molecular structure was similar to a lectin in the biomedical field.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Chemistry, Analytical
Daojin Li, Sihan Dong
Summary: Traditional boronate affinity materials for cis-diol-containing biomolecules suffer from low binding affinity and require a basic binding pH, while 6-aminopyridine-3-boronic acid functionalized magnetic nanoparticles show high binding affinity and a low binding pH. This new material allows for selective extraction of cis-diol-containing biomolecules from samples with low concentrations under neutral or acidic conditions, demonstrating practical applications for enrichment in real samples.
ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Ruoyu Zhao, Binghao Wang, Yan Guo, Jingzi Zhang, Danqi Chen, Wei Ming He, Yong Juan Zhao, Yibing Ding, Chunyuan Jin, Chaojun Li, Yue Zhao, Wei Ren, Lei Fang
Summary: Arsenic pollution has a significant impact on health, particularly in relation to the interaction between SLBP and chaperone complexes like HSPs and ERp44. Knockdown of these proteins can reduce the protein levels of SLBP and inhibit cell migration induced by arsenic exposure.
Article
Multidisciplinary Sciences
Ann Feke, Morgan Vanderwall, Wei Liu, Joshua M. Gendron
Summary: The small LOV/F-box/Kelch family of E3 ubiquitin ligases is essential for regulating plant circadian clocks and flowering time by sensing dusk. Each member of the family has unique protein domains allowing them to act as photoreceptors transducing light signals by altering target protein stability. The Kelch repeat domain of ZTL may mediate inter- and intra-molecular interactions between the three LOV/F-box/Kelch proteins, providing insight into protein complex composition and additional roles of the Kelch repeat domain.
Article
Biochemistry & Molecular Biology
Nader Alerasool, He Leng, Zhen-Yuan Lin, Anne-Claude Gingras, Mikko Taipale
Summary: This study systematically uncovers human proteins that activate transcription in a natural chromatin context. The researchers identified specific co-activators preferred by these transcriptional activators and discovered potent transactivation domains. They also predicted and experimentally validated the interaction interfaces of activation domains with BRD4. Additionally, the study identified novel activators involved in fusion events in tumors and characterized the functional role of one fusion protein.
Article
Oncology
Dan Liu, Chunhui Xu, Yanting Liu, Wen Ouyang, Shaojian Lin, Aining Xu, Yuanliang Zhang, Yinyin Xie, Qiuhua Huang, Weili Zhao, Zhu Chen, Lan Wang, Saijuan Chen, Jinyan Huang, Zhe Bao Wu, Xiaojian Sun
Summary: In this study, a previously unannotated human gene (LY6A) was identified as a candidate ortholog of mouse Ly-6A/Sca-1. It is aberrantly expressed in pituitary tumors and may play a role in tumorigenesis. Similar to its mouse ortholog, human LY6A is upregulated by interferon, indicating a conserved transcriptional regulatory mechanism.
FRONTIERS OF MEDICINE
(2023)
Article
Oncology
Kimihiko Yoshida, Takaki Hiwasa, Masaaki Ito, Mitsunori Ushigome, Hirotaka Takizawa, Shu-Yang Li, Bo-Shi Zhang, Yasuo Iwadate, Kimihiko Funahashi, Hideaki Shimada
Summary: This study evaluated the clinical significance of serum anti-JMJD6 antibodies (s-JMJD6-Abs) in patients with colorectal cancer. The results showed that 37% of colorectal cancer patients tested positive for s-JMJD6-Abs, which may serve as an independent poor prognostic biomarker.
Review
Physics, Applied
Atsushi Kuwahara, Kazunori Ikebukuro, Ryutaro Asano
Summary: Antibody fragments without the Fc region are gaining attention in the pharmaceutical industry due to their ability to penetrate solid tissues, cost-effective expression using microbial systems, and unique modes of action. Various protein engineering strategies, such as high-solubility tag systems, complementarity-determining region grafting, and domain arrangements, have been reported for the cultivation and purification of therapeutic antibody fragments. Careful attention should be paid to the potential negative impact of fusion tags on folding, function, immunogenicity, and yield.
APPLIED PHYSICS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Rebecca A. Oot, Yeqi Yao, Morris F. Manolson, Stephan Wilkens
Summary: V-ATPases are large proton pumps found in virtually every eukaryotic cell, with some isoforms of the a subunit playing a role in tissue-specific diseases and cancer progression. Purification of active human V-ATPases is challenging due to isoform complexity and low abundance, but a strategy involving lipid nanodiscs has been developed for isolation and analysis of the enzyme.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Neurosciences
Celine Nicolas, Rebecca S. Hofford, Emilie Dugast, Virginie Lardeux, Pauline Belujon, Marcello Solinas, Michael T. Bardo, Nathalie Thiriet
Summary: The study found that exposure to environmental enrichment reduces methamphetamine seeking behavior in rats and can reverse methamphetamine-induced increases in glucocorticoid receptor levels in the brain. Additionally, administration of the glucocorticoid receptor antagonist mifepristone significantly decreased methamphetamine seeking.
PSYCHOPHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Stephanie Holm, Anna S. Husted, Louise J. Skov, Thomas H. Morville, Christoffer A. Hagemann, Tina Jorsal, Morten Dall, Alexander Jakobsen, Anders B. Klein, Jonas T. Treebak, Filip K. Knop, Thue W. Schwartz, Christoffer Clemmensen, Birgitte Holst
Summary: This study found that LEAP2 is downregulated during states of energy deprivation in both humans and rodents. The recovery period after endurance exercise is associated with increased levels of BHB and decreased levels of LEAP2 in humans. In fasting and ketogenic diet conditions, Leap2 expression in the liver is decreased. Furthermore, oral administration of BHB and direct exposure to BHB in hepatocytes also decrease LEAP2 levels.
Article
Biochemistry & Molecular Biology
Jens D. Mikkelsen, Sanjay S. Aripaka, Sif Kaad, Burcu A. Pazarlar, Lars Pinborg, Bente Finsen, Andrea Varrone, Benny Bang-Andersen, Jesper F. Bastlund
Summary: A newly developed radioligand [JNJ-64413739] that selectively binds to the purinergic receptor P2X7R has been found to detect neuroinflammation in the brain. The binding properties of JNJ-64413739 in human brain tissue were investigated, and it was found to have different binding abilities in gray and white matter. These findings demonstrate that JNJ-64413739 is suitable for evaluating the distribution and expression of P2X7R in the human brain.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Yan Tong Yang, Shao Wei, Xiaonan Li, Yuanjie Sun, Ping He, Kristi Anne Kohlmeier, Yan Zhu
Summary: Courtship in Drosophila involves dynamic interaction between males and females, with successful copulation depending on the intention of both parties and conveyed through complex action sequences. Recent research focuses on the neural mechanisms controlling female's sexual receptivity. This study reveals that pre-mating sexual receptivity in female Drosophila requires activity within a subset of serotonergic projection neurons, which are regulated by male-derived sex peptide and downstream 5-HT signaling.
Article
Neurosciences
Hoda Ranjbar, Monavareh Soti, Kristi A. Kohlmeier, Mahyar Janahmadi, Mohammad Shabani
Summary: The study evaluated the effects of 3-AP exposure on Purkinje cell electrophysiological properties in cerebellar vermis brain slices. It was found that exposure to 3-AP resulted in significant changes in cellular excitability, but these changes were reversed when treated with a cannabinoid antagonist. This suggests the potential therapeutic use of cannabinoid antagonists in cerebellar dysfunctions.
Article
Developmental Biology
Amin Hassanshahi, Mahyar Janahmadi, Moazamehosadat Razavinasab, Mehran Ilaghi, Kristi A. Kohlmeier, Elham Hassanshahi, Mohammad Shabani
Summary: In this study, the researchers found that prenatal agmatine exposure could ameliorate behavioural and cognitive deficits in female offspring born to prenatally stressed mice. This study contributes to a better understanding of the effects of prenatal stress on newborns and provides a foundation for targeted prenatal treatments.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
(2023)
Article
Cell Biology
Sarah Falk, Jonas Petersen, Charlotte Svendsen, Cesar R. Romero-Leguizamon, Soren Heide Jorgensen, Nathalie Krauth, Mette Q. Ludwig, Kathrine Lundo, Urmas Roostalu, Grethe Skovbjerg, Duy Anh Gurskov Nielsen, Aske Lykke Ejdrup, Tune H. Pers, Oksana Dmytriyeva, Jacob Hecksher-Sorensen, Ulrik Gether, Kristi A. Kohlmeier, Christoffer Clemmensen
Summary: GLP-1R agonists can promote nicotine avoidance and combined treatment with nicotine and GLP-1R agonist can enhance the anti-obesity effects. GLP-1R agonism increases the excitability of hypothalamic POMC neurons and VTA dopaminergic neurons to suppress nicotine-induced dopamine release, resulting in weight loss. These findings support the use of GLP-1R-based therapies for nicotine dependence and further evaluation of combined treatment.
Article
Developmental Biology
Amin Hassanshahi, Mahyar Janahmadi, Moazamehosadat Razavinasab, Hoda Ranjbar, Narges Hosseinmardi, Gila Behzadi, Kristi A. Kohlmeier, Mehran Ilaghi, Mohammad Shabani
Summary: Prenatal stress (PS) can lead to behavioral disorders in offspring, such as substance abuse and anxiety. This study investigated the effects of agmatine on cognitive outcomes in male mice exposed to PS and molecular changes in the ventral tegmental area (VTA). The results showed that agmatine reduced locomotor activity impairment and conditioned place preference to morphine in PS offspring. Agmatine also improved anxiety-like behavior and drug-seeking behavior induced by PS. The molecular effects were observed in the VTA, with agmatine reducing enhanced brain-derived neurotrophic factor (BDNF) levels. Overall, prenatal treatment with agmatine protected against the negative consequences of PS on the development of affective circuits in offspring.
DEVELOPMENTAL PSYCHOBIOLOGY
(2023)
Article
Clinical Neurology
Siri A. Vulpius, Sebastian Werge, Isabella Friis Jorgensen, Troels Siggaard, Jorge Hernansanz Biel, Gitte M. Knudsen, Soren Brunak, Lars H. Pinborg
Summary: This study demonstrates that text mining of clinical notes can improve the accuracy of epilepsy diagnosis and classification of different epilepsy types for patients. It suggests that combining population-based health registries with electronic health records can be crucial for future precision medicine development for epilepsy patients.
Article
Biochemistry & Molecular Biology
Altair Brito Dos Santos, Siganya Thaneshwaran, Lara Kamal Ali, Cesar Ramon Romero Leguizamon, Yang Wang, Morten Pilgaard Kristensen, Annette E. Langkilde, Kristi A. Kohlmeier
Summary: This study found that a-syn(M) has different effects on female LDT neurons compared to male. In females, a-syn(M) decreases membrane excitability and reduces intracellular calcium, which depends on inhibitory acid transmission and leads to decreased amplitude and frequency of sEPSCs, as well as reduced action potential firing rate. It was also found that a-syn(M) induces higher neurodegeneration in males compared to females through inhibitory amino acid transmission. GABA receptor agonists were associated with reduced cell death in males.
CELL AND BIOSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Mina Abghari, Jenny Thythy Cecilia Mai Vu, Ninna Eckberg, Blanca I. Aldana, Kristi A. Kohlmeier
Summary: This study found that glutamate-mediated signaling in CA1 neurons and astrocytes was impaired in a mouse model of Alzheimer's disease, and decanoic acid was able to restore this signaling to normal levels.
Article
Education, Special
Laurits Munk Hojberg, Jesper Lundbye-Jensen, Jacob Wienecke
Summary: This study aimed to investigate how young adults with Down syndrome (DS) learn and retain new motor skills. The results showed that individuals with DS had poorer motor performance compared to typically developed individuals. However, they demonstrated online improvements in performance with practice and showed offline consolidation, leading to significant retention effects.
RESEARCH IN DEVELOPMENTAL DISABILITIES
(2023)
Article
Psychology, Educational
Linn Damsgaard, Anne-Mette Veber Nielsen, Marta Katarzyna Topor, Rasmus Ahmt Hansen, Soren Kildahl Jensen, Rebekka Laessoe Markers, Anne Kaer Gejl, Anne Sofie Bogh Malling, Jacob Wienecke
Summary: The study aimed to investigate the effect of embodied learning on children's literacy skills and whether the activities were particularly beneficial for children at risk for reading difficulties. The results demonstrated that the teaching method combining movement and phonemes significantly improved children's letter knowledge and spelling performance. However, no significant differences were observed between low and high performers, suggesting that this type of activities may be beneficial for all school children at this age.
EDUCATIONAL PSYCHOLOGY REVIEW
(2023)
Article
Chemistry, Medicinal
Christian B. M. Poulie, Camilla B. Chan, Aleksandra Parka, Magnus Lettorp, Josephine Vos, Amanda Raaschou, Eline Pottie, Mikkel S. Bundgaard, Louis M. E. Sorensen, Claudia R. Cecchi, Emil Marcher-Rorsted, Anders Bach, Matthias M. Herth, Ann Decker, Anders A. Jensen, Betina Elfving, Andreas C. Kretschmann, Christophe P. Stove, Kristi A. Kohlmeier, Claus Cornett, Christian Janfelt, Birgitte R. Kornum, Jesper L. Kristensen
Summary: The study found that pellotine, a Lophophora alkaloid, has short-acting sleep-inducing properties and acts through modulation of serotonergic receptors. Pellotine undergoes slow metabolism and readily enters the central nervous system. It was also demonstrated that pellotine is responsible for the hypnotic effects, rather than an active metabolite.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Chemistry, Medicinal
Bala Krishna Prabhala, Jiwan Chettri, Nagalakshmi Irrinki, Abhroop Garg, Rosa Jersie-Christensen, Havard Jenssen, Biljana Mojsoska, Neeraj Soni, Kristi A. Kohlmeier
Summary: As part of the brainstem reticular activating system, the laterodorsal tegmentum (LDT) plays a central role in regulating sleep, arousal, and motivated behaviors including addiction. This study investigated the neuronal lipids within the LDT and the effects of ex vivo treatment with the FAAH inhibitor OTMK. Metabolomics and proteomic analyses were conducted to reveal differences between treated and untreated LDT samples. The results suggest that this ex vivo preparation can be used for screening different FAAH inhibitors and to further characterize the global mass spectrometry-based omics profiles within the LDT.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Pei-Yang Gao, Ya-Nan Ou, Yi-Ming Huang, Zhi-Bo Wang, Yan Fu, Ya-Hui Ma, Qiong-Yao Li, Li-Yun Ma, Rui-Ping Cui, Yin-Chu Mi, Lan Tan, Jin-Tai Yu
Summary: Liver function may play a role in the progression of Alzheimer's disease. The study found that as AD progressed, certain liver function markers increased while others decreased. The relationship between liver function and CSF AD biomarkers indicates a potential mediation effect on cognition.
JOURNAL OF NEUROCHEMISTRY
(2024)