期刊
JOURNAL OF MOLECULAR DIAGNOSTICS
卷 18, 期 3, 页码 378-387出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jmoldx.2015.12.002
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资金
- Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [A111550]
- Korea Health Promotion Institute [A111550] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Until now, fetal placenta specific epigenetic markers for noninvasive prenatal testing of fetal trisomy 21 (T21) have been identified based only on differences in tissue-specific epigenetic characteristics between placenta and maternal blood, but these characteristics have not been validated in T21 placenta. We aimed to discover novel epigenetic markers on chromosome 21 that show a hypermethylated pattern in fetal placenta compared with blood, regardless of the presence of T21. We performed a high-resolution tiling array analysis of chromosome 21 using the methylated-CpG binding domain protein based method. We identified 93 epigenetic regions that showed fetal placenta specific differential methylation patterns; among these, three regions showed fetal placenta specific methylation patterns in T21 placenta samples. The methylation patterns of these three regions in the array were confirmed by bisulfite direct sequencing. The three regions were detectable in first-trimester maternal plasma. Moreover, a combination of their methylation ratio achieved high diagnostic accuracy for noninvasive prenatal testing of fetal T21 by further statistical analysis. These three novel regions with fetal placenta specific differential methylation patterns on chromosome 21 were identified irrespective of the presence of T21. Our findings suggest that epigenetic characteristics of markers according to the presence or absence of T21 should be considered in the development of noninvasive prenatal testing of fetal T21 using fetal placenta specific epigenetic markers.
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