Article
Chemistry, Physical
Elif Ozyilmaz, Sebahat Ascioglu, Mustafa Yilmaz
Summary: Immobilization of enzymes to magnetic metal-organic frameworks enhances enzyme activity, stability, and reusability, with excellent enantioselectivity demonstrated. It may have implications for pharmaceutical industry due to increased reusability and stability, enantioselectivity exhibited by MOF-enzyme biocomposites, and significant differences in biological activities of enantiomers.
Article
Chemistry, Physical
Joanna Chalupka, Adam Sikora, Michal Piotr Marszall
Summary: In this study, enantioselective biotransformation of (R,S)-atenolol was achieved using various catalytic systems containing ionic liquids and toluene as a reaction medium, as well as lipases from Candida rugosa. The use of a two-phase reaction system enabled the reuse of the biocatalyst and resulted in satisfactory kinetic resolution parameters.
Review
Biochemistry & Molecular Biology
Rafael Leandro Fernandes Melo, Misael Bessa Sales, Viviane de Castro Bizerra, Paulo Goncalves de Sousa Junior, Antonio Luthierre Gama Cavalcante, Tiago Melo Freire, Francisco Simao Neto, Muhammad Bilal, Teofil Jesionowski, Joao Maria Soares, Pierre Basilio Almeida Fechine, Jose Cleiton Sousa dos Santos
Summary: Magnetic biocatalysts, combining magnetic properties and enzyme catalytic activity, offer easy recovery and reuse. Lipases immobilized by magnetic nanoparticles dominate research in this field. This review provides a bibliometric analysis and overview of the area, highlighting research advances and hotspots, as well as the diverse applications of magnetic biocatalysts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Joanna Chalupka, Jacek Duleba, Adam Sikora, Tomasz Siodmiak, Michal Piotr Marszall
Summary: In this study, enantioselective biotransformations of (R,S)-1-phenylethanol were performed using various catalytic systems containing ionic liquids and n-heptane or toluene as reaction medium. The use of Burkholderia cepacia lipase, vinyl acetate, and n-heptane with [EMIM][BF4] proved to be effective in obtaining enantiomerically pure 1-phenylethyl acetate, with high enantiomeric excess (ee(p) = 98.9%) and enantioselectivity (E > 200). Furthermore, the use of ionic liquids allowed for the reuse of the enzyme in multiple reaction cycles, ensuring operational stability.
Article
Green & Sustainable Science & Technology
Le Zhong, Xiaobo Jiao, Hongtong Hu, Xuejian Shen, Juan Zhao, Yuxiao Feng, Conghai Li, Yingjie Du, Jiandong Cui, Shiru Jia
Summary: In this study, surfactant activated lipase from Aspergillus oryzae was used to prepare magnetic hybrid nanoflowers with Fe3O4 magnetic nanoparticles embedded, showing high catalytic efficiency, tolerance, and reusability. The magnetic hybrid nanoflowers exhibited excellent performance in producing biodiesel from soybean oil, with a high yield even after multiple cycles of reuse.
Article
Biotechnology & Applied Microbiology
Shushu Wang, Shan Li, Runtang Liu, Wei Zhang, Huajin Xu, Yi Hu
Summary: In this study, Candida rugosa lipase (CRL) was activated and covalently immobilized on a nanocomposite material using surfactants. The nanocomposite material, fabricated by combining magnetic nanoparticles with chitosan, proved to be an ideal support for enzyme immobilization. The immobilized CRL showed improved stability and reusability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Organic
Nilton Goncalves da Cruz, Amanda Silva de Miranda, Henriete da Silva Vieira, Markus Kohlhoff, Joao Guilherme Pereira Mendonca, Marisa Alves Nogueira Diaz, Gaspar Diaz-Munoz
Summary: This paper describes the enantioselective synthesis of the Hancock 1,2,3,4-tetrahydroquinoline alkaloids, including (S)-galipeine, (S)-cuspareine, (S)-galipinine, and (S)-angustureine, as well as the nonnatural enantiomer (R)-galipeine. The target compounds were obtained from a racemic quinaldinic acid derived alpha-amino ester in five steps, with overall yields ranging from 21.2% to 37.5%. The synthesis involved enzymatic kinetic resolution to control the C-2 stereocenter, Wittig olefination for introducing alkyl substituents at C-2, and catalytic hydrogenation to furnish the target alkaloids.
SYNTHESIS-STUTTGART
(2023)
Article
Biophysics
Hongbo Suo, Moju Li, Renmin Liu, Lili Xu
Summary: In this study, imidazolium-based ionic liquid was successfully grafted to magnetic polydopamine nanoparticles for lipase immobilization. The immobilized lipase showed excellent activity and stability, with increased affinity to substrate. Additionally, the immobilized lipase can be easily separated from the reaction system with a magnet, providing new ideas for further studies in this field.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Energy & Fuels
Xiu Xing, Zi-Wen Zhou, Yun-Jie Wei, Yan-Hong Liu, Kun Li, Xiao-Qi Yu, Na Wang
Summary: The process of jatropha oil transesterification for biodiesel production was studied using immobilized Rhizomucor miehei lipase (RML) in ionic liquids. The highest synthetic activity of immobilized RML was found in [BMIM][PF6], which was more than five times higher than the free enzyme. The immobilized enzyme showed good stability even after multiple reuses and storage at -20 degrees C for 98 days.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Energy & Fuels
Fahimeh Esmi, Tahereh Nematian, Zeinab Salehi, Abbas Ali Khodadadi, Ajay K. Dalai
Summary: Fatty acid methyl ester (FAME) was synthesized from olive oil using Rhizopus oryzae lipase (ROL) immobilized on mesoporous silica magnetic nanoparticles (MNPs). The immobilized lipase showed increased enzyme loading efficiency and led to a higher biodiesel production compared to other nanobiocatalysts, making it a suitable nanomaterial for ROL immobilization. The physico-chemical properties of the MNPs were characterized and correlated with their activities for biodiesel synthesis.
Article
Biochemistry & Molecular Biology
Hui Guo, Bingshuang Lei, Jianwei Yu, Yunfei Chen, Junqing Qian
Summary: The study successfully immobilized Rhizopus lipase on magnetic dialdehyde cellulose nanoparticles, optimizing the immobilization conditions to improve enzyme stability and activity. Changes in the secondary structures of the immobilized enzyme revealed increased conformational rigidity, leading to enhanced stability at different temperatures and pH levels, as well as improved tolerance to organic reagents. After storage for 30 days and 6 cycles, the immobilized enzyme still retained high recovery activity, indicating the promising application prospect of MDC in immobilized enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Organic
Qian Chen, Hao Sun, Linlin Li, Jie Tian, Qian Xu, Nana Ma, Li Li, Lin Zhang, Chun Li
Summary: The asymmetric hydrogenation of alpha-halogenated ketones with iridium catalyst using cinchona-alkaloid-based NNP ligands has been developed, allowing the efficient synthesis of chiral halohydrins with a wide substrate scope and high enantioselectivity.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Isabela Oliveira Costa, Nathalia Saraiva Rios, Paula Jessyca Morais Lima, Luciana Rocha Barros Goncalves
Summary: Enzyme immobilization is a method to enhance enzyme application by allowing their reuse and improving stability. The immobilization of enzymes through nanoflowers structures is an innovative and cost-effective technique. This study aims to synthesize hybrid biocatalytic nanostructures similar to flowers using lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL). The production of nanoflowers was achieved through precipitation of lipases with CuCl2 or CuSO4 salts for 72 hours. However, challenges were faced in terms of low thermal stability and reusability. Glutaraldehyde cross-linking was tested to overcome these challenges, resulting in improved thermal stability and efficiency.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hongjun Zhang, Zeguang Cheng, Litian Wei, Xinjun Yu, Zhao Wang, Yinjun Zhang
Summary: In this study, a novel esterase gene was identified and its enantioselectivity was improved through protein engineering. The mutant BaCE-L86Q/G284E showed promising potential as a biocatalyst for the preparation of specific organic acids, as demonstrated by its superior E value.
BIOORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Elif Ozyilmaz, Sebahat Ascioglu, Mustafa Yilmaz
Summary: Metal organic frameworks (MOFs) are hybrid materials with unique properties, used for enzyme immobilization. CRL@ Calix-ZIF-8 shows improved stability and catalytic activity in enzyme immobilization applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Dorota Chelminiak-Dudkiewicz, Aleksander Smolarkiewicz-Wyczachowski, Katarzyna Wegrzynowska-Drzymalska, Marta Ziegler-Borowska
Summary: Levan, a biocompatible and renewable biopolymer with anticancer properties, has promising applications in various industries. However, the literature lacks information on its behavior under UV irradiation, potentially limiting its use. This study investigates the effects of irradiation on the structural properties of levan, and finds that photodegradation occurs faster and more efficiently with a polychromatic light source compared to an LED lamp.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Wegrzynowska-Drzymalska, Kinga Mylkie, Pawel Nowak, Dariusz T. Mlynarczyk, Dorota Chelminiak-Dudkiewicz, Halina Kaczmarek, Tomasz Goslinski, Marta Ziegler-Borowska
Summary: In this study, dialdehyde starch nanocrystals were used as cross-linking agents to chemically cross-link native chitosan, gelatin, and a mixture of these two biopolymers. The resulting materials exhibited desirable properties for biomedical applications, and their characteristics such as hydrophilicity, swelling ability, and protein absorption were also evaluated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Tomasz Siodmiak, Jacek Duleba, Natalia Kocot, Dorota Watrobska-Swietlikowska, Michal Piotr Marszall
Summary: The lipolytic activity of immobilized Lipase Amano A from Aspergillus niger (AA-ANL) was investigated in this study. It was found that the immobilized AA-ANL exhibited significantly higher lipolytic activity compared to the free lipase, and hyperactivation was observed in most tested substrates. The use of different polyacrylic supports for immobilization also showed beneficial effects, and a "lipolytic jump" was observed in the full range of tested temperature and pH.
Article
Biochemistry & Molecular Biology
Katarzyna Wegrzynowska-Drzymalska, Dariusz T. Mlynarczyk, Dorota Chelminiak-Dudkiewicz, Halina Kaczmarek, Tomasz Goslinski, Marta Ziegler-Borowska
Summary: In this study, thin chitosan-gelatin biofilms cross-linked with dialdehyde cellulose nanocrystals were successfully obtained for dressing materials. The selective oxidation of nanocrystals improved the degree of cross-linking and enhanced the performance of the biofilms, such as roughness parameters and antioxidant activity. DNCL showed better cross-linking effects than DAMC. The cross-linking process also improved oxygen permeability and anti-inflammatory properties. Films cross-linked with DAMC exhibited lower toxicity, making them potentially more suitable for medical dressings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Emilia Piosik, Michal Kotkowiak, Anna Modlinska, Dorota Chelminiak-Dudkiewicz, Marta Ziegler-Borowska
Summary: This study successfully synthesized novel nanoagents called ZnPc-MNPs, which have dual anticancer activity and can be used for hyperthermia and photodynamic therapy. Through characterization analysis, it was proven that ZnPc-MNPs have excellent photophysical parameters and high anticancer activity. Additionally, it was shown that ZnPc-MNPs have limited impact on cell membranes and exhibit good biocompatibility.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Biochemistry & Molecular Biology
Aleksander Smolarkiewicz-Wyczachowski, Halina Kaczmarek, Jaroslaw Piskorz, Pawel Nowak, Marta Ziegler-Borowska
Summary: The study focuses on preparing and characterizing photosensitive composites based on chitosan for photodynamic therapy. Chitosan films with a 5% addition of two BODIPY dyes were prepared and their properties were investigated. The addition of BODIPY to chitosan increased hydrophilicity, structure heterogeneity, and roughness. The presence of iodine atoms in the BODIPY structure caused a bathochromic effect and decreased the emission quantum yield in the composites. BODIPY-doped chitosan showed improved interactions with human serum albumin and acidic alpha-glycoprotein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Dorota Chelminiak-Dudkiewicz, Aleksander Smolarkiewicz-Wyczachowski, Kinga Mylkie, Magdalena Wujak, Dariusz T. Mlynarczyk, Pawel Nowak, Szymon Bocian, Tomasz Goslinski, Marta Ziegler-Borowska
Summary: This study focuses on obtaining and characterizing novel chitosan-based biomaterials containing cannabis oil to potentially promote wound healing. The study found that increasing the concentration of cannabis oil in chitosan-based films improved their roughness, mechanical parameters, swelling capacity, hydrophilic properties, and antimicrobial effect. However, it also decreased water vapor permeability. The films were found to be biocompatible and could be potential candidates for wound dressing materials.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Joanna Chalupka, Jacek Duleba, Adam Sikora, Tomasz Siodmiak, Michal Piotr Marszall
Summary: In this study, enantioselective biotransformations of (R,S)-1-phenylethanol were performed using various catalytic systems containing ionic liquids and n-heptane or toluene as reaction medium. The use of Burkholderia cepacia lipase, vinyl acetate, and n-heptane with [EMIM][BF4] proved to be effective in obtaining enantiomerically pure 1-phenylethyl acetate, with high enantiomeric excess (ee(p) = 98.9%) and enantioselectivity (E > 200). Furthermore, the use of ionic liquids allowed for the reuse of the enzyme in multiple reaction cycles, ensuring operational stability.
Article
Biochemistry & Molecular Biology
Joanna Chalupka, Adam Sikora, Marta Ziegler-Borowska, Michal Piotr Marszall
Summary: The aim of this study was to obtain (S)-clopidogrel through the enantioselective biotransformation of clopidogrel carboxylic acid and chiral chromatographic separation using a lipase from Candida rugosa and a two-phase reaction medium containing an ionic liquid. The best kinetic resolution parameters were obtained using lipase from Candida rugosa OF, cyclohexane and [EMIM][BF4] as the reaction medium, and methanol as the acyl acceptor. The study proved the possibility of obtaining (S)-clopidogrel in a parallel with green chemistry methodology and environmentally friendly conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Aleksander Smolarkiewicz-Wyczachowski, Halina Kaczmarek, Jaroslaw Piskorz, Marta Ziegler-Borowska
Summary: In this study, chitosan films modified by photosensitive BODIPY compounds with and without iodine atoms were prepared and their stability under thermal and UV radiation conditions was investigated. The results showed that BODIPY dyes exhibited good photochemical stability in solid chitosan matrix, but the compound with iodine atoms showed slightly lower stability compared to the one without. Additionally, BODIPY dyes did not affect the thermal stability of chitosan, but their decomposition temperature was lower in the presence of chitosan.
Article
Environmental Sciences
Piotr Macczak, Halina Kaczmarek, Marta Ziegler-Borowska
Summary: The study investigated the potential use of chitosan and its derivatives, dialdehyde chitosan and carboxymethyl chitosan, as alternative flocculants for purifying filter backwash water. The modified chitosan derivatives were successfully synthesized and characterized using various techniques. The biopolymer flocculants showed promising efficiency in removing impurities, with carboxymethyl chitosan achieving nearly 99% iron removal and 98.26% turbidity removal rates at an optimal dosage of 0.2 mg/L.
Review
Chemistry, Multidisciplinary
Piotr Szyk, Beata Czarczynska-Goslinska, Dariusz T. Mlynarczyk, Barbara Slusarska, Tomasz Kocki, Marta Ziegler-Borowska, Tomasz Goslinski
Summary: This article reviews the scientific progress and prospects of polymer-based nanoparticles as drug delivery systems for purines. These nanoparticles have the potential to enhance the therapeutic effects of purine derivatives by specific binding, increased drug retention and penetration, and can also reduce the toxic side effects of these drugs.
Article
Biochemistry & Molecular Biology
Dorota Chelminiak-Dudkiewicz, Miloslav Machacek, Jolanta Dlugaszewska, Magdalena Wujak, Aleksander Smolarkiewicz-Wyczachowski, Szymon Bocian, Kinga Mylkie, T. Goslinski, Michal P. Marszall, Marta Ziegler-Borowska
Summary: In this study, porous bacterial levan-based sponges containing cannabis oil (Lev@CBDs) were prepared and characterized. The sponges exhibited desirable physical properties and showed good performance in terms of swelling ratio, water vapor transmission rate, thermal stability, mechanical properties, antioxidant and anti-inflammatory properties. These sponges also demonstrated antibacterial activity and biocompatibility. The results suggest that Lev@CBDs have the potential to be used as wound dressings in future in vivo applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Emilia Piosik, Michal Kotkowiak, Anna Modlinska, Dorota Chelminiak-Dudkiewicz, Marta Ziegler-Borowska
Summary: This study presents a novel nanoagent with dual anticancer activity based on magnetite nanoparticles coated with aminated chitosan and immobilized with zinc(II) phthalocyanine. The synthesized nanoagent combines the superparamagnetic properties of magnetite nanoparticles and the photosensitizing potential of zinc(II) phthalocyanine, making it a promising material for dual anticancer therapy based on hyperthermia and photodynamic effects.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Engineering, Environmental
Rafal Krakowiak, Robert Frankowski, Kinga Mylkie, Michal Kotkowiak, Dariusz T. Mlynarczyk, Alina Dudkowiak, Beata J. Stanisz, Agnieszka Zgola-Grzeskowiak, Marta Ziegler-Borowska, Tomasz Goslinski
Summary: Hybrid materials based on nanometric size bare anatase titanium(IV) oxide and meso-functionalized porphyrins were prepared and characterized. The hybrid materials showed higher photocatalytic activity than bare titanium(IV) oxide in the degradation of ibuprofen under UV light and had relatively good photocatalytic activity under visible light.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
