期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 128, 期 -, 页码 65-72出版社
ELSEVIER
DOI: 10.1016/j.molcatb.2016.03.012
关键词
Deep eutectic solvents; Solvatochromic parameters; Lipase; Stability
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A01060206]
- Korea CCS RD Center (KCRC) [2014M1A8A1049296]
- Rural Development Administration through the Cooperative Research Program for Agriculture Science & Technology Development [010205022014]
- National Research Foundation of Korea [2015R1D1A1A01060206] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
DESs (deep eutectic solvents) have many potential applications as cosolvents or anhydrous reaction media for biocatalytic reactions, owing to their non-volatility, non-flammability, non-toxicity, biocompatibility, biodegradability, and low cost. In this work, choline chloride ([Ch]Cl)-based DESs and DES mixtures containing two hydrogen bond donors were used as cosolvents to enhance the activity and stability of Candida rugosa lipase in aqueous reactions. The activity of lipase in an aqueous solution of [Ch]Cl:urea:glycerol was 155% higher than that in buffer. The half-life time of lipase at 40 degrees C in an aqueous solution of [Ch]Cl:glycerol was enhanced by 9.2 times. The lipase showed the highest acid stability and base stability in the aqueous solutions of [Ch]Cl:glycerol:thiourea and [Ch]Cl:ethylene glycol:formamide, respectively. In general, glycerol-containing DES mixtures were very useful in enhancing the activity and stability of lipase, while formamide-containing DES mixtures could not efficiently enhance the activity and stability of lipase. To understand the effect of DES mixtures on the activity and stability of lipase in aqueous solution, four solvatochromic parameters of DES mixtures were determined. When the solvatochromic parameters of DES mixtures were correlated with the stability of lipase in aqueous solutions of DES mixtures, it was found that thermal stability and storage stability of lipase were associated with the hydrogen bond acidity of DES mixtures. Acid stability and base stability of lipase were correlated with polarity based on Reichardt's dye and the dipolarity/polarizability of DES mixtures, respectively. (C) 2016 Elsevier B.V. All rights reserved.
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