期刊
JOURNAL OF MICROSCOPY
卷 263, 期 2, 页码 200-211出版社
WILEY
DOI: 10.1111/jmi.12424
关键词
Osmium impregnation; plant membranes; SBFSEM; selective staining
类别
资金
- BBSRC [BB/L014122/1]
- ERACAPs/BBSRC [BB/M000168/1]
- BBSRC [BB/M000168/1, BB/D524567/1, BB/L014122/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L014122/1, BB/M000168/1, BB/D524567/1] Funding Source: researchfish
Serial block face imaging with the scanning electron microscope has been developed as an alternative to serial sectioning and transmission electron microscopy for the ultrastructural analysis of the three-dimensional organization of cells and tissues. An ultramicrotome within the microscope specimen chamber permits sectioning and imaging to a depth of many microns within resin-embedded specimens. The technology has only recently been adopted by plant microscopists and here we describe some specimen preparation procedures suitable for plant tissue, suggested microscope imaging parameters and discuss the software required for image reconstruction and analysis.
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