期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 126, 期 -, 页码 38-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2016.04.016
关键词
Infection biology; Semi-quantitative western blot; Data normalization; Stain-Free technology
资金
- DFG [JI 221/1-1]
- FoFoLe program of the Medical Faculty of the LMU [26/2013]
Western blots are a commonly used method for protein detection and quantification in biological samples. Compensation of loading variations is achieved by housekeeping protein (HKP) normalization and/or total protein normalization (TPN). However, under infection conditions, HKP normalization, traditionally used in cell biology for quantification of western blots, can be problematic. Binding of microbes to target cells via specific receptors can induce signal transduction events resulting in drastic changes in the level of expression of HKPs. Additionally, samples collected after infection assays will include cellular and microbial proteins altering the analysis with TPN. Here we demonstrate under experimental infection conditions, how a reliable semi-quantitative analysis of proteins in western blots can be achieved using the Stain-Free technology. (C) 2016 Elsevier B.V. All rights reserved.
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