Article
Environmental Sciences
Yu Zhang, Zheyu Li, Yanxiang Zhang, Kai Sun, Nanqi Ren, Mingtang Li
Summary: This study investigated the effects of diclofenac exposure on adult male crayfish. The results showed histological changes in the hepatopancreas and abnormal gene expressions related to antioxidant, immune, and metabolic functions. The intestinal microbiota of the crayfish was also significantly altered after diclofenac exposure.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Yu Zhang, Zheyu Li, Xing Tian, Pianpian Xu, Kai Sun, Nanqi Ren
Summary: This study investigates the toxic effects of MC-LR on crayfish. The results show that MC-LR can effectively accumulate in the hepatopancreas, gills, and intestines of crayfish. Antioxidant-related genes show different expressions in response to MC-LR-induced oxidative stress. MC-LR also leads to histological changes and disruptions in the intestinal flora of crayfish. These findings provide new insights into the toxic impacts of microcystins on aquatic crustaceans.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Fisheries
Honglian Chen, Yongjie Wang, Jing Zhang, Junjie Bao
Summary: This study investigated the intestinal microbiota of healthy crayfish, WSSV-infected active crayfish, and WSSV-infected diseased crayfish using high throughput sequencing technology. The results showed that WSSV infection led to significant changes in certain phyla in the intestines of crayfish, with implications for developing microbial strategies for the prevention and therapy of WSS disease.
Article
Microbiology
Mengqi Xie, Shiyu Zhang, Lili Xu, Zhixin Wu, Junfa Yuan, Xiaoxuan Chen
Summary: This study used high-throughput sequencing to analyze the intestinal microbiota of Procambarus clarkii at different growth stages. The results showed that alpha diversity decreased with crayfish growth, with significant differences in microbiota composition among stages. The microbiota exhibited a consistent progression pattern from larval to juvenile stage, indicating changes in composition and function as the crayfish developed.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Xiaowen Zhang, Manli Shen, Cui Wang, Miaomiao Gao, Liuen Wang, Zeyu Jin, Xiaohua Xia
Summary: This study evaluated the effects of aluminum exposure on crayfish. The results showed that aluminum induced biochemical, histological, gut microbial, and immune changes in the crayfish. Therefore, excessive aluminum exposure may have adverse effects on various biological processes of crayfish, posing a potential threat to crayfish culture.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Shengxuan Li, Yanbin Lin, Naijuan He, Liu Fang, Qian Wang, Guoliang Ruan
Summary: This study investigated the effects of low-temperature aerial exposures on the antioxidative and immune responses and hepatopancreatic histopathology in P. clarkii. The results showed that low-temperature exposure caused severe oxidative damage and reduced antioxidant capacity in crayfish. However, 12 hours of re-immersion was not sufficient to restore crayfish from stress to a normal state.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2023)
Article
Fisheries
Lei Zhu, Yiming Kong, Xulu Chang, Junchang Feng, Xinru Wang, Libo Hou, Xianliang Zhao, Chao Pei, Xianghui Kong
Summary: The study demonstrated that fish-derived probiotics can improve the growth performance, immune response, intestinal microbiota diversity, and pathogen resistance of crayfish, suggesting their potential as feed additives in aquaculture.
Article
Environmental Sciences
Rong Han, Aman Khan, Zhenmin Ling, Ying Wu, Pengya Feng, Tuoyu Zhou, El-Sayed Salama, Marwa M. El-Dalatony, Xirong Tian, Pu Liu, Xiangkai Li
Summary: In this study, Limosilactobacillus fermentum GR-3 strain isolated from water was found to have high adsorption capacity for Arsenic (As) and strong antioxidant abilities. When added to crayfish feed, the strain effectively reduced the concentration of As(III) and restored gut microbiota dysbiosis caused by As(III) exposure. The application of this strain in aquaculture also resulted in a decrease in As(III) accumulation and increased production.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Environmental Sciences
Wang Lin, Jingyi Wu, Huimin Luo, Xiangli Liu, Beibei Cao, Fen Hu, Fang Liu, Jifeng Yang, Pinhong Yang
Summary: Ammonia, a major water pollutant in aquaculture, has negative effects on the antioxidant and innate immune responses in crustaceans. This study investigated these effects in red swamp crayfish exposed to different concentrations of ammonia. The results showed that high concentrations of ammonia induced hepatopancreatic injury and suppressed the antioxidant capacity and innate immunity of P. clarkii.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Agriculture, Dairy & Animal Science
Hui Xu, Xuexia Bai, Yu Li, Jiajia Li, Yong Meng, Zhiqiang Xu, Jianqing Tang, Yan Lu, Yahong Huang
Summary: Freshwater biodiversity crisis has attracted significant attention due to the destruction of freshwater ecosystems. Drought, as an important factor contributing to this crisis, is still poorly understood. In this study, we examined the immune function, histopathology, and metabolism of crayfish under drought conditions using flow cytometry, hematoxylin-eosin staining, and untargeted metabolomics. The results demonstrate that drought suppresses immune function, disrupts the balance of oxidative and antioxidative systems, and induces tissue damage and metabolic changes in crayfish.
Article
Green & Sustainable Science & Technology
Md Muzammel Hossain, Yuan Yuan, Hengliang Huang, Ziwei Wang, Mohammad Abdul Baki, Zhihua Dai, Muhammad Rizwan, Shuanglian Xiong, Menghua Cao, Shuxin Tu
Summary: The study investigated the effects of D6 exposure on crayfish, revealing changes in oxidative stress induction and gene expression. It showed alterations in enzyme activities, antioxidant systems, and related gene expression in crayfish under D6 exposure. The research provides insights into the toxicity mechanism of D6 in crayfish and serves as a basis for further exploration in this area.
Article
Immunology
Minghao Li, Jincheng Wang, Huiling Deng, Liangyu Li, Xiaoli Huang, Defang Chen, Ping Ouyang, Yi Geng, Shiyong Yang, Lizi Yin, Wei Luo, Jun Jiang
Summary: This study investigates the effects of C. freundii on the digestive organs of crayfish and reveals that it disrupts intestinal microbiota, leading to inflammation and integrity disruption. C. freundii infection also stimulates bile acid biosynthesis and upregulates lipid degradation and cytochrome P450-related pathways in the hepatopancreas. The disrupted intestinal-liver axis homeostasis may serve as a potential basis for C. freundii pathogenesis in crayfish.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Rossy Guillen-Watson, Maria Arias-Andres, Keilor Rojas-Jimenez, Ingo S. Wehrtmann
Summary: Microplastics are a hazardous pollutant that can cause dysbiosis of the intestinal microbiome and impair the immune system of crayfish, as observed in this study using Procambarus clarkii as a model.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Honglin Chen, Fangfang Liu, Miaofeng Ouyang, Huan Zhou, Bao Lou
Summary: This study investigated the intestinal microbiota of two important freshwater crayfish species, the red swamp crayfish (Procambarus clarkii, Pc) and the red claw crayfish (Cherax quadricarinatus, Cq). The results showed that Pc had higher community richness and diversity compared to Cq, suggesting its stronger environmental adaptability. The core phyla identified in Pc were Proteobacteria, Tenericutes, Bacteroidetes, Firmicutes, and RsaHF231, while Tenericutes, Proteobacteria, and Firmicutes were detected in Cq. Proteobacteria exhibited significant differential abundance between males and females in both Pc and Cq. Additionally, Vibrio coralliilyticus was found particularly in the intestine of Cq.
Article
Fisheries
Hui Yang, Tongwei Ji, Haoran Xiong, Yingying Zhang, Wenzhi Wei, Qiuning Liu
Summary: This study investigated the immune response of Procambarus clarkii to WSSV infection and analyzed the expression of hematopoietic cytokines. Transcriptome analysis revealed differentially expressed genes and their involvement in various biological processes and signaling pathways. Real-time quantitative PCR analysis validated the findings of transcriptome analysis. The results of this study provide new evidence for the theoretical basis of the crayfish immune system and offer new insights for disease prevention and control.
FISH & SHELLFISH IMMUNOLOGY
(2022)