Article
Multidisciplinary Sciences
Cheng-Yang Zhang, Xiao-Feng Zhang, Jun Yuan, Yuan-Feng Gong, Hui Tang, Wan-Yu Guo, Tong-Yan Li, Cai-Wen Li, Yun-Qiang Tang, Ning-Fang Ma, Ming Liu
Summary: This study successfully optimized the culture procedure for HCC organoids, establishing a culture protocol that allows for the formation of larger-sized HCC organoids with stable passaging and culture conditions. Detailed instructions and precautions were provided for each step of the procedure, from HCC tissue dissociation to cryopreservation and resuscitation. These organoids exhibit genetic similarity to original HCC tissues and can be utilized for various applications, including the identification of potential therapeutic targets and drug development.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Cell Biology
Jana Zschuentzsch, Stefanie Meyer, Mina Shahriyari, Karsten Kummer, Matthias Schmidt, Susann Kummer, Malte Tiburcy
Summary: This article summarizes the recent progress in preclinical models for neuromuscular diseases, with a focus on the application of in vitro models in studying disease mechanisms and therapeutic strategies. The integration of multicellular interactions of muscle tissue in vitro and its impact on disease phenotypes is highlighted.
Article
Biophysics
Hao Nguyen Tran, Vini Gautam
Summary: Brain organoids are powerful models for studying neurodevelopmental processes and neurological disorders, but their limited use in biomedicine and clinical research due to complex cellular architecture and lack of vascular networks can be overcome by micro/nanoscale devices. These devices can improve in vitro culture methods, enable physiological recordings, screen drugs, study drug effects, and develop vascular networks in organoids, advancing the potential of brain organoids in clinical research.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Biotechnology & Applied Microbiology
Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Summary: Cellular phenotypes and functional responses are regulated by signals present in their microenvironment. High throughput culture platforms can better recapitulate and analyze these complex signals. Various techniques such as microplates, microarrays, and microfluidic cell culture systems have been developed for high throughput cell culture. Current applications include stem cell sourcing, drug discovery, and personalized medicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kohta Toshimitsu, Ai Takano, Masayuki Fujii, Kazuhiro Togasaki, Mami Matano, Sirirat Takahashi, Takanori Kanai, Toshiro Sato
Summary: This study establishes a drug screening platform for patient-derived colorectal cancer organoids and identifies the bromodomain and extra-terminal bromodomain protein inhibitor as a cancer-selective growth suppressor. It also discovers an association between checkpoint with forkhead and ring finger domaines silencing and paclitaxel sensitivity.
NATURE CHEMICAL BIOLOGY
(2022)
Review
Genetics & Heredity
Toshikatsu Matsui, Tadahiro Shinozawa
Summary: Organoids, three-dimensional structures fabricated in vitro, mimic complex microenvironments and physiological functions, bridging the gap between animals and humans in research. In toxicology and drug development, human organoids offer new possibilities by overcoming limitations of current models.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Soon-Chan Kim, Ji Won Park, Ha-Young Seo, Minjung Kim, Jae-Hyeon Park, Ga-Hye Kim, Ja Oh Lee, Young-Kyoung Shin, Jeong Mo Bae, Bon-Kyoung Koo, Seung-Yong Jeong, Ja-Lok Ku
Summary: The study reveals the widespread subregional heterogeneity within single tumors, leading to varied drug responses, emphasizing the importance of understanding the molecular heterogeneity of each tumor and targeting common somatic driver mutations positioned in all tumor subregional clones.
Review
Biochemistry & Molecular Biology
Tingyu Fang, Hua Wang, Xiaoyue Pan, Peter J. Little, Suowen Xu, Jianping Weng
Summary: The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing every year, but there are currently no approved drugs for its treatment. Mouse models are commonly used to study NAFLD and can simulate different stages of the disease, playing an important role in research and drug discovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Tingting Tao, Pengwei Deng, Yaqing Wang, Xu Zhang, Yaqiong Guo, Wenwen Chen, Jianhua Qin
Summary: A new microfluidic multi-organoid system has been developed to replicate the human liver-pancreatic islet axis in both normal and disease states, providing a unique platform for future research and drug development in Type 2 diabetes mellitus.
Review
Cell Biology
Marlene Geyer, Karla Queiroz
Summary: PDAC, the most common type of pancreatic cancer, is characterized by early metastasis and often requires chemotherapy treatment. With only 20% of tumors resectable, there is an urgent need for innovative modeling platforms to better understand and treat this complex disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Jingyu Fu, Hailong Qiu, Cherie S. Tan
Summary: Drug discovery is a costly and complex process, and efficient methods are needed to screen potential compounds and remove toxic substances. The liver plays a crucial role in determining drug efficacy and potential side effects. The liver-on-a-chip platform based on microfluidic technology has gained attention for its ability to predict drug metabolism and hepatotoxicity, as well as study pharmacokinetics and pharmacodynamics when combined with other organ-on-chip systems.
Review
Pharmacology & Pharmacy
Jie Zhang, Hamed Tavakoli, Lei Ma, Xiaochun Li, Lichun Han, XiuJun Li
Summary: Cancer immunotherapy has achieved remarkable success, but the resistance to treatment remains a challenge. Understanding the interactions between the tumor microenvironment (TME) and the immune system is crucial in developing effective immunotherapies. Microfluidic tumor organoid-on-a-chip platforms have emerged as innovative tools to investigate the immune response to tumors and resistance to immunotherapy. This review focuses on recent advances and applications of these platforms.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Biotechnology & Applied Microbiology
Kayque Alves Telles-Silva, Lara Pacheco, Sabrina Komatsu, Fernanda Chianca, Luiz Carlos Caires-Junior, Bruno Henrique Silva Araujo, Ernesto Goulart, Mayana Zatz
Summary: The liver is the most important metabolic hub for endo and xenobiotic compounds. However, current in vitro models have limitations in predicting clinical outcomes. This review discusses the two most robust liver in vitro models, organoids and liver-on-chip, including their design, manufacturing techniques, cellular composition and functionality. The efficiency of drug screening and liver disease modeling is summarized. Finally, the advantages and limitations of organoids and liver-on-chip technologies are compared to guide appropriate model selection and technology development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Chrysanthi-Maria Moysidou, Chiara Barberio, Roisin Meabh Owens
Summary: Research in cell biology heavily relies on in vitro assays and models, with the recent advancement in 3D cell biology and engineering providing more physiologically relevant culture platforms. These platforms, such as scaffold-based systems and organs-on-chips, show great potential in bridging the gap between conventional animal studies and human physiology by allowing for more accurate modeling of specific processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Gastroenterology & Hepatology
Charles J. Zhang, Sophia R. Meyer, Matthew J. O'Meara, Sha Huang, Meghan M. Capeling, Daysha Ferrer-Torres, Charlie J. Childs, Jason R. Spence, Robert J. Fontana, Jonathan Z. Sexton
Summary: In this study, the utility of human liver organoids (HLOs) for high-throughput drug-induced liver injury (DILI) risk prediction and organ-on-chip system was evaluated. The results showed that HLOs have similar DILI predictive capacity as intact HLOs and can predict hepatotoxicity associated with DILI. The organ-on-chip system exhibits in vivo-like functions and is beneficial for DILI risk assessment.
JOURNAL OF HEPATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
John H. Ahrens, Sebastien G. M. Uzel, Mark Skylar-Scott, Mariana M. Mata, Aric Lu, Katharina T. Kroll, Jennifer A. Lewis
Summary: This study reports the fabrication of aligned cardiac tissues using bioprinting technology. By printing anisotropic organ building blocks, functional cardiac tissues with high cell density and complex cellular alignment can be generated.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Jay M. Taylor, Haiwen Luan, Jennifer A. Lewis, John A. Rogers, Ralph G. Nuzzo, Paul Braun
Summary: This article highlights recent progress in soft material chemistry and enabling methods of 3D and 4D fabrication. It discusses the emerging programmable material designs and associated assembly methods for constructing complex functional structures. The ability to programmably vary soft material architectures with properties and shapes over time is emphasized, particularly focusing on biomimetic and biologically compliant soft materials. The challenges and prospects for integrating chemistry, form, and function in this field are described, with examples of biomimetic and biologically compliant behaviors.
ADVANCED MATERIALS
(2022)
Review
Cell & Tissue Engineering
Kayla J. Wolf, Jonathan D. Weiss, Sebastien G. M. Uzel, Mark A. Skylar-Scott, Jennifer A. Lewis
Summary: The construction of human organs on demand is a solution to address the shortage of organ donors. However, replicating the complexity of native organs remains a significant challenge. The use of organ building blocks offers a pathway to create organ-specific tissues with desired organization.
Article
Cell Biology
Navin Gupta, Takuya Matsumoto, Ken Hiratsuka, Edgar Garcia Saiz, Pierre Galichon, Tomoya Miyoshi, Koichiro Susa, Narihito Tatsumoto, Michifumi Yamashita, Ryuji Morizane
Summary: Kidneys have the capacity for intrinsic repair, but when overwhelmed, incomplete repair can lead to chronic kidney disease. Limited treatments exist due to the limited predictivity of animal studies. This study used kidney organoids to identify genes and pathways involved in intrinsic repair and discovered a potential therapeutic candidate that can prevent the progression of chronic kidney disease.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Min Shi, Kyle W. McCracken, Ankit B. Patel, Weitao Zhang, Lioba Ester, M. Todd Valerius, Joseph Bonventre
Summary: This study describes a highly efficient method for differentiating human pluripotent stem cells into kidney collecting duct cells. The process involves inducing the stem cells into progenitor cells, forming organoids with similar morphology to fetal kidney, and ultimately differentiating into functional collecting duct cells.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jeffrey O. Aceves, Szilvia Heja, Kenichi Kobayashi, Sanlin S. Robinson, Tomoya Miyoshi, Takuya Matsumoto, Olivier J. M. Schaffers, Ryuji Morizane, Jennifer A. Lewis
Summary: This study presents a method for creating a perfusable 3D proximal tubule model that exhibits improved drug uptake. The model shows upregulation of transporters and increased lactate dehydrogenase release when exposed to nephrotoxic substances. This platform holds promise for personalized kidney-on-chip models for drug screening and disease modeling.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Benito Roman-Manso, Robert D. Weeks, Ryan L. Truby, Jennifer A. Lewis
Summary: Embedded 3D printing combined with microwave-activated curing is used to generate architected ceramics with spatially controlled composition in freeform shapes. This integrated manufacturing method opens new avenues for the design and fabrication of complex ceramic architectures with programmed composition, density, and form for myriad applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Robert D. D. Weeks, Ryan L. L. Truby, Sebastien G. M. Uzel, Jennifer A. A. Lewis
Summary: Recent advancements in computational design and 3D printing technology have allowed for the fabrication of polymer lattices that have a high strength-to-weight ratio and tailored mechanics. However, limitations with most commercial 3D printing platforms have only allowed for the construction of monolithic 3D lattices. This study demonstrates the use of embedded three-dimensional (EMB3D) printing to create multi-material polymer lattices, enabling the fabrication of a wide range of periodic and stochastic lattices with tailored mechanical responses by printing multiple materials with different mechanical properties.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Natalie M. Larson, Jochen Mueller, Alex Chortos, Zoey S. Davidson, David R. Clarke, Jennifer A. Lewis
Summary: This article presents a rotational multimaterial 3D printing (RM-3DP) platform that allows subvoxel control over the local orientation of azimuthally heterogeneous architected filaments. By continuously rotating a multimaterial nozzle with a controlled ratio of angular-to-translational velocity, helical filaments with programmable helix angle, layer thickness, and interfacial area between different materials can be created. Functional artificial muscles and hierarchical lattices composed of architected helical struts have been successfully fabricated using this method.
Article
Materials Science, Multidisciplinary
Bradley Duncan, Robert D. Weeks, Benjamin Barclay, Devon Beck, Patrick Bluem, Roberto Rojas, Maxwell Plaut, John Russo, Sebastien G. M. Uzel, Jennifer A. Lewis, Theodore Fedynyshyn
Summary: In this study, low-loss graded dielectric materials were successfully designed and printed using active mixing of nanocomposite inks. This method enables the rapid fabrication of high-performance RF devices with locally tunable dielectric properties.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Cell Biology
Rolando Carrisoza-Gaytan, Katharina T. Kroll, Ken Hiratsuka, Navin R. Gupta, Ryuji Morizane, Jennifer A. Lewis, Lisa M. Satlin
Summary: Kidney organoids cultured with flow exhibit more mature podocytes and tubules compared to static controls. However, their physiological function needs further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Review
Biology
Ran Konoe, Ryuji Morizane
Summary: Organoids are small 3D models of organs that aid in studying their function and diseases. Kidney organoids have the potential to treat chronic kidney disease, which affects over 800 million individuals. However, creating fully functional kidney organoids is difficult due to immaturity and the absence of blood vessels and tissue organization.
Article
Materials Science, Multidisciplinary
Connor A. Verheyen, Sebastien G. M. Uzel, Armand Kurum, Ellen T. Roche, Jennifer A. Lewis
Summary: This study combines experimentation and computation to design granular matrices with controlled properties, allowing for potential applications in cell encapsulation, bioprinting, and tissue engineering.