Endonuclease-Assisted PAM-free Recombinase Polymerase Amplification Coupling with CRISPR/Cas12a (E-PfRPA/Cas) for Sensitive Detection of DNA Methylation

Development of PCR assays to detect signature circulating tumor DNA methylation markers and KRas mutations for pancreatic ductal adenocarcinoma (PDAC).

(2022) Shiwei Guo et al.   JOURNAL OF CLINICAL ONCOLOGY

A Simple and Highly Sensitive Naked-Eye Analysis of EGFR 19del via CRISPR/Cas12a Triggered No-Nonspecific Nucleic Acid Amplification

(2022) Chunfeng Feng et al.   ACS Synthetic Biology

CRISPR technologies and the search for the PAM-free nuclease

(2021) Daphne Collias et al.   Nature Communications

Sensitive, Rapid, and Automated Detection of DNA Methylation Based on Digital Microfluidics

(2021) Qingyu Ruan et al.   ACS Applied Materials & Interfaces

Combined recombinase polymerase amplification/rkDNA–graphene oxide probing system for detection of SARS-CoV-2

(2021) Moon Hyeok Choi et al.   ANALYTICA CHIMICA ACTA

The push and pull of DNA methylation

(2021) Tianpeng Gu et al.   SCIENCE

A novel DNA methylation biosensor by combination of isothermal amplification and lateral flow device

(2021) Xiaonan Liu et al.   SENSORS AND ACTUATORS B-CHEMICAL

Evaluating the Consistency of Gene Methylation in Liver Cancer Using Bisulfite Sequencing Data

(2021) Xubin Zheng et al.   Frontiers in Cell and Developmental Biology

Digital Loop-Mediated Isothermal Amplification-Based Absolute Methylation Quantification Revealed Hypermethylated DAPK1 in Cervical Cancer Patients

(2021) Ya-Ling Tan et al.   ANALYTICAL CHEMISTRY

Navigating the DNA methylation landscape of cancer

(2021) Atsuya Nishiyama et al.   TRENDS IN GENETICS

Dual Methylation-Sensitive Restriction Endonucleases Coupling with an RPA-Assisted CRISPR/Cas13a System (DESCS) for Highly Sensitive Analysis of DNA Methylation and Its Application for Point-of-Care Detection

(2021) Xianfeng Wang et al.   ACS Sensors

Microfluidic Ruler-Readout and CRISPR Cas12a-Responded Hydrogel-Integrated Paper-Based Analytical Devices (μReaCH-PAD) for Visible Quantitative Point-of-Care Testing of Invasive Fungi

(2021) Di Huang et al.   ANALYTICAL CHEMISTRY

A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites

(2021) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

Live-Cell Probe for In Situ Single-Cell Monitoring of Mitochondrial DNA Methylation

(2021) Han Zhao et al.   ACS Sensors

HOGG1-assisted DNA methylation analysis via a sensitive lanthanide labelling strategy

(2021) Jing Zhou et al.   TALANTA

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage

(2020) Yijuan Bao et al.   ACS Sensors

Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities

(2020) Jeanne E. van Dongen et al.   BIOSENSORS & BIOELECTRONICS

Recent advances in biosensors for in vitro detection and in vivo imaging of DNA methylation

(2020) Qian Zhang et al.   BIOSENSORS & BIOELECTRONICS

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

(2020) Hua Liu et al.   FOOD CHEMISTRY

Comparison of Bisulfite Pyrosequencing and Methylation-Specific qPCR for Methylation Assessment

(2020) Loretta De Chiara et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Use of DNA methylation profiling in translational oncology

(2020) Vanessa Ortiz-Barahona et al.   SEMINARS IN CANCER BIOLOGY

EPIC-TABSAT: analysis tool for targeted bisulfite sequencing experiments and array-based methylation studies

(2019) Julie Krainer et al.   NUCLEIC ACIDS RESEARCH

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Highly sensitive detection of sentinel lymph node metastasis of breast cancer by digital PCR for RASSF1A methylation

(2019) Mizuho Abe et al.   ONCOLOGY REPORTS

Bandage-like wearable flexible microfluidic recombinase polymerase amplification sensor for the rapid visual detection of nucleic acids

(2019) Bin Yang et al.   TALANTA

Rolling Circular Amplification (RCA)-Assisted CRISPR/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs

(2019) Ruixuan Wang et al.   ANALYTICAL CHEMISTRY

Endonuclease-assisted hydrogel bead array for digital analysis of circulating tumor DNA methylation

(2019) Yunlong Liu et al.   SENSORS AND ACTUATORS B-CHEMICAL

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Structural Basis for the Altered PAM Specificities of Engineered CRISPR-Cas9

(2016) Seiichi Hirano et al.   MOLECULAR CELL