Article
Obstetrics & Gynecology
F. Vilella, W. Wang, I Moreno, B. Roson, S. R. Quake, C. Simon
Summary: Analysis of single-cell RNA-sequencing in 73,181 human endometrial cells from 27 donors shows low expression of ACE2 in stromal or unciliated epithelial cells across the menstrual cycle, indicating a low risk of SARS-CoV-2 endometrial infection in healthy reproductive-age women.
HUMAN REPRODUCTION
(2021)
Article
Immunology
Jennifer L. Welch, Jinhua Xiang, Qing Chang, Jon C. D. Houtman, Jack T. Stapleton
Summary: SARS-CoV-2 infection may express its receptor ACE-2 in T cells, altering T cell proliferation, activation, and survival, leading to impaired immune function.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Cell Biology
Rajasekaran Mahalingam, Prakash Dharmalingam, Abirami Santhanam, Sivareddy Kotla, Gangarao Davuluri, Harry Karmouty-Quintana, Guha Ashrith, Rajarajan A. Thandavarayan
Summary: This study analyzed the expression of SARS-CoV-2 virus in human organoid cells using single-cell RNA sequencing data, finding abundant expression of ACE2 and TMPRSS2 in most organoids. It was discovered that low-density lipoprotein receptor expression is enriched in intestinal, lung, and retinal organoid cells. The study demonstrates that organoids can serve as an experimental platform for investigating the disease mechanism of this novel virus and for drug development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Virology
James Harte, Samantha L. Wakerlin, Andrew J. Lindsay, Justin McCarthy, Caroline Coleman-Vaughan
Summary: This study found that metalloproteases play an important role in the pathomechanism of COVID-19, promoting the fusion and syncytiation of SARS-CoV-2 spike protein. Inhibitors of metalloproteases may be beneficial in the treatment of COVID-19 patients.
Article
Chemistry, Multidisciplinary
Taylor F. Gunnels, Devin M. Stranford, Roxana E. Mitrut, Neha P. Kamat, Joshua N. Leonard
Summary: The study demonstrates that cell-mimicking decoy nanoparticles can effectively inhibit SARS-CoV-2 and its variants, providing a promising approach to overcome drug resistance challenges and offering significant implications for potential clinical applications.
Article
Cell Biology
Thankamani Karthika, Jeswin Joseph, V. R. Akshay Das, Niranjana Nair, Packirisamy Charulekha, Melvin Daniel Roji, V. Stalin Raj
Summary: The cytoplasmic tail of ACE2 is not essential for the entry of SARS-CoV-1 and -2, suggesting that their entry may be mediated via known or unknown host factors. Inhibition of pseudotyped SARS-CoVs entry into cells was observed when treated with a dynamin inhibitor and an endosomal acidification inhibitor. Antibodies against SARS-CoV and soluble ACE2 were unable to enter cells expressing wtACE2 and increment cytACE2.
Article
Pharmacology & Pharmacy
Scott P. Davies, Courtney J. Mycroft-West, Isabel Pagani, Harriet J. Hill, Yen-Hsi Chen, Richard Karlsson, Ieva Bagdonaite, Scott E. Guimond, Zania Stamataki, Marcelo Andrade De Lima, Jeremy E. Turnbull, Zhang Yang, Elisa Vicenzi, Mark A. Skidmore, Farhat L. Khanim, Alan Richardson
Summary: The study suggests that fenofibrate and fenofibric acid may be effective in reducing SARS-CoV-2 infection by up to 70% at clinically achievable concentrations. These drugs have a history of clinical use and relatively good safety profiles, making them potential therapeutic agents that require urgent clinical evaluation for treating SARS-CoV-2 infection.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Virology
Soheila Kazemi, Alberto Domingo Lopez-Munoz, Jaroslav Holly, Ling Jin, Jonathan W. Yewdell, Brian P. Dolan
Summary: The study presents a method to generate cells stably expressing different orthologs of ACE2 receptor for SARS-CoV-2 and found that both S-protein RBD binding and pseudovirus infection are proportional to ACE2 levels at the cell surface. This approach allows the creation of a library of stably transfected cells expressing different vertebrate ACE2 orthologs, useful for identifying susceptible vertebrate species for SARS-CoV-2 infection and its variants.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Christopher J. Day, Benjamin Bailly, Patrice Guillon, Larissa Dirr, Freda E. -C. Jen, Belinda L. Spillings, Johnson Mak, Mark von Itzstein, Thomas Haselhorst, Michael P. Jennings
Summary: This study identified compounds that bind to human ACE2 or the SARS-CoV-2 spike protein receptor binding domain through molecular docking and SPR screening, with three compounds demonstrating dose-dependent antiviral potency in vitro.
Article
Acoustics
D. Cui, Y. Liu, X. Jiang, C. Ding, L. C. Poon, H. Wang, H. Yang
Summary: This study found the presence of ACE2- and TMPRSS2-positive cells in the human trophectoderm and placenta in all three trimesters of pregnancy, suggesting the possibility of SARS-CoV-2 spreading through the placenta and causing intrauterine fetal infection.
ULTRASOUND IN OBSTETRICS & GYNECOLOGY
(2021)
Article
Oncology
Christophe Deben, Maxim Le Compte, Vasiliki Siozopoulou, Hilde Lambrechts, Christophe Hermans, Ho Wa Lau, Manon Huizing, Kevin Lamote, Jeroen M. H. Hendriks, Peter Van Dam, Patrick Pauwels, Evelien L. J. Smits, Marc Peeters, Filip Lardon
Summary: SARS-CoV-2 infection can occur in non-small-cell lung cancer (NSCLC) patients who express ACE2, TMPRSS2, and FURIN on the surface of their lung cancer cells. Increased expression of ACE2 is correlated with higher levels of soluble ACE2 (sACE2) in the serum. Standard of care (SOC) therapies do not increase sACE2 levels. The study suggests that EGFR mutation is associated with higher expression of ACE2 and sACE2 in NSCLC patients, and it is important to further investigate the impact of mACE2, sACE2, and SOC targeted therapies on the course of COVID-19.
Article
Pharmacology & Pharmacy
Siew Pheng Lim
Summary: Despite the availability of vaccines and therapeutics, continuous genetic alterations in the SARS-CoV-2 virus pose a persistent threat, especially to immunocompromised and elderly individuals. The virus enters host cells via the interaction of its spike protein (S protein) with different receptors on the cell surface, such as angiotensin-converting enzyme 2 (ACE2). This review discusses therapeutic approaches to block the interaction between SARS-CoV-2 and host cell receptors, as well as the identification of auxiliary entry receptors.
ANTIVIRAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Zengyuan Zhang, Yanfang Zhang, Kefang Liu, Yan Li, Qiong Lu, Qingling Wang, Yuqin Zhang, Liang Wang, Hanyi Liao, Anqi Zheng, Sufang Ma, Zheng Fan, Huifang Li, Weijin Huang, Yuhai Bi, Xin Zhao, Qihui Wang, George F. Gao, Haixia Xiao, Zhou Tong, Jianxun Qi, Yeping Sun
Summary: The study found that the spike protein receptor binding domain (RBD) of SARS-CoV-2 can bind to the receptor of dog angiotensin-converting enzyme 2 (dACE2), allowing both pseudotyped and authentic SARS-CoV-2 to infect cells expressing dACE2. Additionally, important mutations in the RBD binding interface are identified to play a pivotal role in the binding affinity of RBD to both dACE2 and hACE2, providing insights into potential animal spread and cross-species transmission of SARS-CoV-2.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Linjie Li, Hanyi Liao, Yumin Meng, Weiwei Li, Pengcheng Han, Kefang Liu, Qing Wang, Dedong Li, Yanfang Zhang, Liang Wang, Zheng Fan, Yuqin Zhang, Qiyue Wang, Xin Zhao, Yeping Sun, Niu Huang, Jianxun Qi, George Fu Gao
Summary: The study reveals the differences in binding affinities between the four early sub-variants of the Omicron variant and human ACE2 receptor, providing insights into the structural basis for these differences.
Article
Biochemistry & Molecular Biology
Satya Prakash Shukla, Kwang Bog Cho, Vineeta Rustagi, Xiang Gao, Xinping Fu, Shaun Xiaoliu Zhang, Bin Guo, D. Gomika Udugamasooriya
Summary: The study identified two ACE2-binding peptoid compounds that effectively block the entry of SARS-CoV-2 into human cells. These compounds also showed efficacy against a mutant virus and were found to be safe without reducing ACE2 expression or enzyme activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Qi Yao, Xinqi Ge, Zhichao Lu, Jinlong Shi, Jianhong Shen, Jian Chen
Summary: In this study, the researchers investigated the role and prognostic value of HAUS1 in glioma, a type of brain tumor. They found that HAUS1 was upregulated and served as a biomarker for poor prognosis in patients with glioma. High HAUS1 expression was associated with several tumor-infiltrating immune cells, suggesting that HAUS1 plays a pivotal role in immune infiltration in glioma. The findings suggest that HAUS1 could be a potential biomarker for predicting the prognosis of patients with glioma.
JOURNAL OF ONCOLOGY
(2022)
Article
Cell Biology
Ziheng Wang, Xinqi Ge, Jinlong Shi, Bing Lu, Xiaojin Zhang, Jianfei Huang
Summary: The expression of SPTSSA is significantly upregulated in glioma and associated with poor prognosis. The study identifies biological processes and signaling pathways associated with SPTSSA expression, such as oxidative stress. The immunohistochemistry confirms the significant correlation between SPTSSA expression, tumor-infiltrating immune cells, and overall survival.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Pharmacology & Pharmacy
Zongheng Liu, Long Peng, Yidan Sun, Zhichao Lu, Bing Wu, Weichen Wang, Xiaomei Zhang, Haiyan Hao, Peipei Gong
Summary: This study investigated the predictive and therapeutic significance of COMMD4 in glioma. The findings revealed a correlation between the high expression of COMMD4 and unfavorable prognosis in glioma patients. Additionally, COMMD4 was associated with immune cells and drug resistance. It was suggested that COMMD4 could be used as a prognostic marker for glioma.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Weichen Wang, Zhichao Lu, Maoyu Wang, Zongheng Liu, Bing Wu, Chengkai Yang, He Huan, Peipei Gong
Summary: By analyzing cuproptosis-related genes, a cuproptosis-based signature was constructed, revealing that patients with high CuproptosisScore had worse prognosis and higher gene mutation frequency. CuproptosisScore could serve as an independent prognostic factor, providing predictive value for glioma patients and potentially guiding personalized treatment strategies.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Xinqi Ge, Manyu Xu, Tong Cheng, Nan Hu, Pingping Sun, Bing Lu, Ziheng Wang, Jian Li
Summary: This study reveals the association between TP53I13 and gliomas, indicating that TP53I13 expression may impact the survival outcomes in glioma patients. Additionally, TP53I13 serves as an independent marker that plays a crucial role in regulating the infiltration of immune cells into tumors.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Min He, Gujie Wu, Ziheng Wang, Kuan Ren, Zheng Yang, Qun Xue
Summary: This study identified 17 differentially expressed TRP-related genes between lung adenocarcinoma (LUAD) and normal lung tissues using TCGA data. Based on these genes, LUAD patients were classified into two subtypes, which showed significant differences in prognosis, clinical features, and immune cell infiltration characteristics. A prognostic signature with 12 genes was established, and patients were classified into low- and high-risk groups. The low-risk group had significantly longer survival time, which was validated in other datasets. The TRP score was found to be an independent predictor of overall survival in LUAD patients.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Xing Huang, Zhichao Lu, Min He, Yipeng Feng, Shaorong Yu, Bo Shen, Jianwei Lu, Pingping Wu, Banzhou Pan, Hanlin Ding, Chen Chen, Yidan Sun
Summary: Lung adenocarcinoma, a common and aggressive type of lung cancer, has poor prognosis. Investigating the clinical value of oxidative stress mechanisms in regulating tumor cell apoptosis is important. Few studies have examined the impact of the microenvironment on immune-checkpoint inhibitor therapy in LUAD patients. This study develops and validates a gene signature model of oxidative stress-related prognostic markers, which is a promising tool for prognosis identification and immunotherapy.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Zhichao Lu, Yixun Chen, Siqi Chen, Xingjia Zhu, Chenxing Wang, Ziheng Wang, Qi Yao
Summary: This study systematically evaluated the expression differences of NECAP2 in low-grade glioma and pan-cancer. The findings showed that NECAP2 levels were elevated in gliomas, and this upregulation increased with higher tumor grades. NECAP2 overexpression in glioma patients was associated with a higher risk of malignant behavior and worse prognosis. The study also explored the correlation between NECAP2 and cancer immune infiltration, suggesting that NECAP2 could serve as a prognostic biomarker for glioma patients.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Chemistry, Multidisciplinary
Yuejuan Ling, Dekang Nie, Yue Huang, Mengyuan Deng, Qianqian Liu, Jinlong Shi, Siguang Ouyang, Yu Yang, Song Deng, Zhichao Lu, Junling Yang, Yi Wang, Rongqin Huang, Wei Shi
Summary: In this study, a bioactive zeolitic imidazolate framework (ZIF)-8-capped superoxide dismutase (SOD) and Fe3O4 nanoparticles (SOD&Fe3O4@ZIF-8, SFZ) were developed to enhance analgesic efficacy. SFZ nanoparticles showed antioxidant and anti-inflammatory effects, reducing reactive oxygen species (ROS) and inflammatory factors. Additionally, SFZ nanoparticles inhibited the activation of the MAPK/p-65 signaling pathway, preventing microglia and astrocyte activation for pain relief.
Article
Neurosciences
Ziheng Wang, Zhichao Lu, Yixun Chen, Chenxing Wang, Peipei Gong, Rui Jiang, Qianqian Liu
Summary: The aim of this study was to evaluate the effect of epicatechin on neurological recovery and neuroinflammation after traumatic brain injury (TBI) and investigate its potential value in clinical practice. A TBI model was established in adult rats, and the effect of epicatechin was assessed. The results showed that administering epicatechin after TBI prevented neuronal death, reduced neuroinflammation, and promoted neurological function restoration in TBI rats. A network pharmacology study suggested that epicatechin may exert its therapeutic benefits through the AKT-P53/CREB pathway.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Pharmacology & Pharmacy
Xiang Huang, Ziheng Wang, Mengruo Song, He Huan, Zishu Cai, Bing Wu, Jianhong Shen, You Lang Zhou, Jinlong Shi
Summary: In this study, the expression of circRNA (circIQGAP1) was found to be significantly downregulated in IDH1 mutant gliomas, and low circIQGAP1 expression was associated with better prognosis. circIQGAP1 regulated glioma cell behaviors through miR-1256/RCAN1/Bax/Bcl-2/Caspase3 and miR-622/RCAN2/Bax/Bcl-2/Caspase3 axes. The findings suggest that circIQGAP1 plays an important role in glioma development and may serve as a therapeutic target.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Health Care Sciences & Services
Haiyan Hao, Xinyu Yang, Huixia Zhu, Ziheng Wang, Haiyan Zhang, Chunxia Huang
Summary: This study evaluated the effects of intervention through a whole seamless connection of nursing from the WeChat interactive platform on stigma and quality of life in patients with urinary system cancer. The results showed that this intervention could significantly reduce stigma and improve the patients' quality of life.