Article
Biochemical Research Methods
Zahir Ali, Edith Sanchez, Muhammad Tehseen, Ahmed Mahas, Tin Marsic, Rashid Aman, Gundra Sivakrishna Rao, Fatimah Saeed Alhamlan, Madain S. Alsanea, Ahmed A. Al-Qahtani, Samir Hamdan, Magdy Mahfouz
Summary: Bio-SCAN is a rapid and accurate pathogen detection platform for SARS-CoV-2, offering high sensitivity and efficiency in distinguishing between different variants.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, Weihua Guan
Summary: A novel SARS-CoV-2 detection strategy, integrating a solid-state CRISPR-Cas12a-assisted nanopore sensing approach, was developed with a nanopore-sized counting method and a kinetic cleavage model to achieve precise detection of viral RNA, demonstrating trade-offs between sensitivity, specificity, and detection limit.
Article
Chemistry, Multidisciplinary
Ju Zhang, Airu Zhu, Miao Mei, Jing Qu, Yalan Huang, Yongshi Shi, Meiying Xue, Jingfang Zhang, Renli Zhang, Bing Zhou, Xu Tan, Jincun Zhao, Yu Wang
Summary: RNA aptamers are useful biological probes and therapeutic agents. CRISmers is a CRISPR/Cas-based screening system for identifying specific aptamers targeting SARS-CoV-2 variants. The study demonstrates that aptamers selected by CRISmers show sensitive detection and potent neutralization of SARS-CoV-2, both in vitro and in vivo. The findings highlight the robustness, consistency, and potential broad utility of CRISmers.
Review
Virology
Wenjie Jiang, Wangquan Ji, Yu Zhang, Yaqi Xie, Shuaiyin Chen, Yuefei Jin, Guangcai Duan
Summary: The mutations of SARS-CoV-2 have led to a decline in vaccine effectiveness and detection sensitivity, highlighting the importance of research on detecting VOCs for controlling the epidemic.
Article
Immunology
Iqbal Azmi, Md Imam Faizan, Rohit Kumar, Siddharth Raj Yadav, Nisha Chaudhary, Deepak Kumar Singh, Ruchika Butola, Aryan Ganotra, Gopal Datt Joshi, Gagan Deep Jhingan, Jawed Iqbal, Mohan C. Joshi, Tanveer Ahmad
Summary: A major bottleneck in scaling-up COVID-19 testing is the need for sophisticated instruments and trained healthcare professionals, as well as constraints in the supply chain. CASSPIT introduces a new approach that allows direct use of saliva samples for detection without the extra RNA extraction step.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nasir Javaid, Thuong L. H. Pham, Sangdun Choi
Summary: Reversing the transcriptional status of genes using CRISPR/dCas9-based activators has proven to be useful for various purposes. Our study demonstrates that dCas9-VP192 is more effective than VP64-dCas9-VP64 in activating reference genes, resulting in higher mRNA and protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yanan Tang, Turun Song, Lu Gao, Saifu Yin, Ming Ma, Yun Tan, Lijuan Wu, Yang Yang, Yanqun Wang, Tao Lin, Feng Li
Summary: Here, we report an ultrasensitive CRISPR-based antibody detection (UCAD) assay that translates the detection of anti-SARS-CoV-2 antibodies into CRISPR-based nucleic acid detection in a homogeneous solution and is 10,000 times more sensitive than the classic immunoassays. The clinical validation using serum samples shows that UCAD has 100% sensitivity and 98.5% specificity. With ultrahigh sensitivity, UCAD enables the quantitative analysis of serum anti-SARS-CoV-2 levels in vaccinated kidney transplant recipients who are shown to produce undetectable anti-SARS-CoV-2 using standard immunoassay.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Lin Wang, Junhu Zhou, Qixue Wang, Yunfei Wang, Chunsheng Kang
Summary: This study proposes a potential anti-SARS-CoV-2 strategy based on the CRISPR-Cas13a system, identifying a specific RNA segment of the spike protein and designing an efficient crRNA sequence for cleavage. It provides a rapid design pipeline for an antiviral tool against SARS-CoV-2 and introduces a novel approach for anti-virus study.
Article
Microbiology
Yuanhao Liang, Hongqing Lin, Lirong Zou, Jianhui Zhao, Baisheng Li, Haiying Wang, Jing Lu, Jiufeng Sun, Xingfen Yang, Xiaoling Deng, Shixing Tang
Summary: This study developed a CRISPR-Cas12-based system for the direct detection of SARS-CoV-2 variants, achieved high sensitivity, and successfully distinguished specific mutations in different variants. The approach has the potential for rapid development and implementation in resource-limited settings.
MICROBIOLOGY SPECTRUM
(2021)
Article
Nanoscience & Nanotechnology
Lina Wu, Xinjie Wang, Xiangchuan Wu, Shiqi Xu, Ming Liu, Xizhong Cao, Taishan Tang, Xingxu Huang, He Huang
Summary: The CRISPR-Cas system has been developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity. This study demonstrates the use of a MnO2 nanozyme-mediated CRISPR-Cas12a system for the detection of SARS-CoV2. The system shows high selectivity and facile operation, making it a promising molecular diagnosis tool for point-of-care testing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Tong Zhang, Wang Zhao, Xi Chen, Xinlian Zhang, Jinhui Zhu, Shenwei Li, Chuanyong Wu, Zhengan Tian, Guodong Sui
Summary: In this study, a fully automated CRISPR-LAMP platform is proposed, which can precisely manipulate the CRISPR-LAMP droplet and perform combined reactions with high sensitivity and specificity. The platform shows good performance in monitoring SARS-CoV-2 variant mutations and wild type.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Joon Soo Park, Kuangwen Hsieh, Liben Chen, Aniruddha Kaushik, Alexander Y. Trick, Tza-Huei Wang
Summary: The digital-enhanced CRISPR/Cas-assisted one-pot virus detection (deCOViD) is a novel assay that allows qualitative and quantitative detection of SARS-CoV-2 in a very short time with high sensitivity and a broad dynamic range. It outperforms current benchtop-based counterparts and represents one of the fastest and most sensitive CRISPR/Cas-assisted SARS-CoV-2 detection methods to date.
Article
Biochemistry & Molecular Biology
Na Liu, Ran Liu, Jingjing Zhang
Summary: This article introduces a CRISPR-Cas12a-based electrochemical sensor for quantitative detection of SARS-CoV-2 nucleocapsid antigen. The sensor combines aptamer-specific recognition with CRISPR-Cas12a-mediated signal amplification, demonstrating excellent analytical performance.
BIOELECTROCHEMISTRY
(2022)
Article
Biophysics
Woong Heo, Kyungyeon Lee, Sunyoung Park, Kyung-A Hyun, Hyo-Il Jung
Summary: A electrochemical biosensor based on CRISPR/Cas13a was developed for the rapid and sensitive detection of SARS-CoV-2 without the need for nucleic acid amplification. The biosensor showed high sensitivity and a wide detection range, making it suitable for on-site rapid diagnostic testing.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Jen-Hui Tsou, Hongjie Liu, Sanford A. Stass, Feng Jiang
Summary: This study developed a rapid CRISPR test for sensitive detection of SARS-CoV-2 without the need for RNA extraction, allowing for completion within one hour. The test is simple to operate, requiring minimal equipment, and results can be visualized immediately using a UV light illuminator or paper strips.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).