期刊
JOURNAL OF MAMMALIAN EVOLUTION
卷 24, 期 4, 页码 465-474出版社
SPRINGER
DOI: 10.1007/s10914-016-9358-5
关键词
Pygmy hippo; Common hippo; Phylogeny; Painting probes; BAC; Chromosome
资金
- CEITEC - Central European Institute of Technology from the European Regional Development Fund [ED1.1.00/455 02.0068]
- Ministry of Agriculture of the Czech Republic [RO 0516]
The family Hippopotamidae is comprised of two genera with two living species, the common hippo (Hippopotamus amphibius) and the pygmy hippo (Choeropsis liberiensis). Unlike the common hippo, the karyotype of C. liberiensis has not yet been investigated via cross-species chromosome painting methods. We established chromosomal homologies between the pygmy hippo, pig, and cattle by fluorescence in situ hybridization using whole chromosome, arm-specific, region specific, and bacterial artificial chromosome (BAC) probes. Probes from the 18 pig autosomes painted 45 conserved chromosomal segments in the pygmy hippo genome. The pygmy hippo and cattle homology map was deduced from our hybridization results of painting probes to pygmy hippo chromosomes with a combination of previously published dromedary hybridization data. On the pygmy hippo and cattle homology map, 29 cattle autosomes revealed 39 conservative segments on pygmy hippo chromosomes. For a more detailed structural analysis of genome rearrangements and X chromosome structure, we used cattle region specific and BAC probes. Our report demonstrates that cattle probes are useful not only in comparative studies within Ruminantia, but also in more phylogenetically distant Artiodactyla species.
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