Article
Immunology
Ming Yun Yu, Hui Jie Jia, Jing Zhang, Guang He Ran, Yan Liu, Xiu Hong Yang
Summary: Macrophages are immune cells that are highly diverse and flexible, and play a crucial role in fighting against pathogens and tumor cells. They can polarize into M1 phenotype to exert pro-inflammatory effects or M2 phenotype to exert anti-inflammatory effects in response to different stimuli. The balance of macrophage polarization is closely associated with disease progression, and therapeutic approaches targeting macrophage polarization are feasible.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Wenhui Huang, Yue Zhang, Bojun Zheng, Xuguang Ling, Guozhen Wang, Lijuan Li, Wei Wang, Miaoxia Pan, Xu Li, Ying Meng
Summary: Macrophages infiltration plays a crucial role in Sepsis-associated acute lung injury (ALI). This study reveals that Lipopolysaccharides-induced macrophages-derived exosomes (L-Exo) can induce ALI and damage alveolar epithelial cells. It is found that GBP2 in L-Exo targets NLRP3 inflammasomes and promotes inflammation. These findings provide new insights into the prevention and treatment of ALI.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Microbiology
Zhicheng Sun, Xiaoyang Pang, Xiyang Wang, Hao Zeng
Summary: This study aimed to investigate the differential expression of miRNA in macrophage-derived exosomes within the tuberculosis-infected bone microenvironment, and identify potential diagnostic markers and therapeutic targets. Through miRNA-mRNA co-expression network analysis, GO and KEGG pathway enrichment analysis, miRNA-125b-5p was found to be significantly up-regulated in spinal tuberculosis and showed potential as a diagnostic biomarker. The construction of the miRNA-125b-5p target gene network and KEGG enrichment analysis highlighted the importance of MAPK, TNF, Ras, Rap1, and the PI3K-Akt signaling pathways in regulating the disease course of spinal tuberculosis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yunyi Xie, Le Yu, Zhilan Cheng, Yingying Peng, Zeyuan Cao, Beichen Chen, Yihong Duan, Yan Wang
Summary: Human exfoliated deciduous teeth (SHED)-derived exosomes have a therapeutic effect on wound closure and itch relief, making them a potential resource for cell-free therapeutics.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Yeojin Hong, Jiae Lee, Thi Hao Vu, Sooyeon Lee, Hyun S. Lillehoj, Yeong Ho Hong
Summary: Exosomes derived from poly(LC)-activated macrophages stimulate expression of interferons, cytokines, and chemokines in HD11 and CU91 cells, while also inducing the NF-KB signaling pathway. This suggests that chicken macrophages regulate immune responses of naive macrophages and T cells through Exosomes and the NF-KB pathway, making poly(LC)-activated exosomes potentially useful as immunostimulators.
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Review
Neurosciences
Ze-Yan Liang, Xiong-Jie Xu, Jian Rao, Zhe-Lun Yang, Chun-Hua Wang, Chun-Mei Chen
Summary: This review discusses the research progress of MSC-derived exosomal miRNAs in promoting M2 macrophage differentiation in SCI. Research has shown the potential therapeutic effects of exosomal miRNAs in promoting nerve function recovery in SCI patients.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Biochemistry & Molecular Biology
V. B. Sameer Kumar, K. Anjali
Summary: One of the major reasons for the non-effectiveness of anticancer therapeutics targeting tumour angiogenesis is the complexity of cellular communication in the tumour micro-environment. Recent research has highlighted the importance of signaling events mediated by exosomes in tumor progression and angiogenesis. This review collates information on the role of exosomal cargo, especially exosomal miRNAs, in modulating tumor angiogenesis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Oncology
America Campos, Shayna Sharma, Andreas Obermair, Carlos Salomon
Summary: The article discusses the association between miRNAs within extracellular vesicles (EVs) and chemotherapy resistance, identifying certain miRNAs in various cancers linked to chemoresistance. Studies suggest that miR-21, miR-222, and miR-155 have roles in response to chemotherapy in different types of tumors.
Article
Pharmacology & Pharmacy
Mengying Niu, Hong Li, Xu Li, Xiaoqian Yan, Aijun Ma, Xudong Pan, Xiaoyan Zhu
Summary: This study identified four promising exosomal miRNAs associated with large-artery atherosclerosis (LAA) stroke, the most common subtype of ischemic stroke, through high-throughput sequencing and qRT-PCR analysis. These miRNAs can serve as diagnostic biomarkers for LAA stroke, showing superior diagnostic efficiency compared to their plasma counterparts.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Oncology
Xiaoyun He, Gaoyan Kuang, Yongrong Wu, Chunlin Ou
Summary: Exosomes are small vesicles secreted by cells and can carry various cargo including RNA, DNA, proteins, and metabolites. Studies have shown that exosomal microRNAs play a crucial role in the progression of diseases such as diabetes mellitus, affecting processes like pancreatic beta-cell injury and insulin resistance. Understanding the mechanisms of exosomal miRNAs in diabetes mellitus can provide valuable insights for identifying diagnostic biomarkers and drug targets.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Lianyuan Tao, Deyu Li, Sengmao Mu, Guanjing Tian, Guoyi Yan
Summary: Our study reveals that the hepatitis-B antigen HBeAg found in HBV+HCC upregulates long non-coding RNA MAPKAPK5_AS1 (MAAS) expression in M2 macrophages by affecting its m6A modification. MAAS is transferred to HBV+HCC cells via exosomes, which in turn facilitates their proliferation. This is a novel role for MAAS and further elucidates the mechanism of HBeAg-induced HBV-related HCC development.
LABORATORY INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Zunyong Feng, Jing Zhou, Yinhua Liu, Ruixue Xia, Qiang Li, Liang Yan, Qun Chen, Xiaobing Chen, Yuxin Jiang, Gao Chao, Ming Wang, Guoren Zhou, Yijie Zhang, Yongsheng Wang, Hongping Xia
Summary: This study investigated the role of RGS1 in regulating the immunophenotype of different subpopulations of alveolar macrophages, finding that miRNA in exosomes can affect their immune balance, with potential therapeutic effects on ALI/ARDS and PF.
CELL DEATH AND DIFFERENTIATION
(2021)
Review
Oncology
Chaoliang Liao, Huiwen Liu, Xiangjian Luo
Summary: Nasopharyngeal carcinoma is a unique subtype of head and neck cancer endemic to Southern China and Southeast Asia, with most patients diagnosed in advanced stages and having poor prognosis. Exosomal miRNAs play a crucial role in the tumorigenesis, development, and treatment of NPC, serving as valuable biomarkers.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Thi Hao Vu, Yeojin Hong, Anh Duc Truong, Jiae Lee, Sooyeon Lee, Ki-Duk Song, Jihye Cha, Hoang Vu Dang, Ha Thi Thanh Tran, Hyun S. Lillehoj, Yeong Ho Hong
Summary: In this study, the gene expression related to cytokine-cytokine receptor interactions in resistant and susceptible chickens infected with avian influenza virus was investigated. The results showed that resistant chickens exhibited stronger immune responses and antiviral activity than susceptible chickens against HPAIV infection.
Article
Agriculture, Dairy & Animal Science
Thi Hao Vu, Yeojin Hong, Anh Duc Truong, Sooyeon Lee, Jubi Heo, Hyun S. Lillehoj, Yeong Ho Hong
Summary: Resistant Ri chickens show higher antiviral activity compared to susceptible Ri chickens, and H5N1-infected resistant Ri chickens exhibit immune responses and antiviral activity through the MAPK signaling pathway.
Article
Fisheries
Yeojin Hong, Anh Duc Truong, Thi Hao Vu, Sooyeon Lee, Jubi Heo, Suyeon Kang, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study demonstrates the immunomodulatory effects of exosomes from HPAIV H5N1-infected chickens on immune-related cells, showing that these exosomes induce pro-inflammatory cytokine expression and stimulate the MAPK signaling pathway. Moreover, viral proteins from H5N1 are packaged in exosomes and transferred to non-infected immune-related cells.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Anh Duc Truong, Suyeon Kang, Hoang Vu Dang, Yeojin Hong, Thi Hao Vu, Jubi Heo, Nhu Thi Chu, Huyen Thi Nguyen, Ha Thi Thanh Tran, Yeong Ho Hong
Summary: In this study, 27 differentially expressed miRNAs were identified in the serum of African swine fever virus (ASFV)-infected pigs. These miRNAs were found to be associated with immune response, virus-host interactions, and several viral genes. This study provides important insights into the pathogenesis of ASFV and the host's immune response during an acute infection.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Veterinary Sciences
Thi Hao Vu, Jubi Heo, Yeojin Hong, Suyeon Kang, Ha Thi Thanh Tran, Hoang Vu Dang, Anh Duc Truong, Yeong Ho Hong
Summary: Highly pathogenic avian influenza viruses (HPAIVs) have high infectivity and mortality rates in chickens, causing significant economic impact on the poultry sector. This study aimed to elucidate the pathogenic mechanism of HPAIV through gene set enrichment analysis (GSEA) comparing resistant and susceptible chicken lines. The results showed that resistant chickens exhibited stronger antiviral responses to HPAIV infection. This study provides insights into the immune responses of genetically disparate chickens against HPAIV.
JOURNAL OF VETERINARY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Anh Duc Truong, Ha Thi Thanh Tran, Huyen Thi Nguyen, Yeong Ho Hong, Hyun S. Lillehoj, Hoang Vu Dang, Ki-Duk Song, Nhu Thi Chu
Summary: This study identified and characterized the chicken interleukin-1 receptor type 2 (chIL-1R2) in chicken immunity. The chIL-1R2 protein can specifically bind to chicken IL-1f3. The expression of chIL-1R2 can be induced by viral infection and regulated through NF-kB/JNK/MEK-mediated signaling pathways.
Article
Agriculture, Dairy & Animal Science
Yeojin Hong, Jubi Heo, Suyeon Kang, Thi Hao Vu, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study evaluated the target genes and immune responses regulated by gga-miR-20a-5p in the chicken macrophage cell line HD11 through exosome-mediated delivery. Exosomes were purified from HD11 cells and loaded with gga-miR-20a-5p mimic or negative control miRNA. The exosome-mediated delivery of gga-miR-20a-5p successfully suppressed the expression of target genes, interferon-stimulated genes, and proinflammatory cytokines, while promoting cell proliferation in HD11 cells.
Article
Agriculture, Dairy & Animal Science
Anh Duc Truong, Ha Thi Thanh Tran, Nhu Thi Chu, Huyen Thi Nguyen, Thi Hao Vu, Yeojin Hong, Ki-Duk Song, Hoang Vu Dang, Yeong Ho Hong
Summary: In this study, 20 FGF genes were identified in chickens and their roles in embryo development and immune responses were investigated. The study revealed interactions between FGF genes and MAPK genes, and their involvement in stimulating signaling pathways and regulating immune responses. The findings provide valuable insights into the functions of FGFs in chickens.
Article
Agriculture, Dairy & Animal Science
Sooyeon Lee, Suyeon Kang, Jubi Heo, Yeojin Hong, Thi Hao Vu, Anh Duc Truong, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study analyzed the miRNA expression profiles in resistant and susceptible lines of Ri chickens infected with HPAI H5N1 virus. The results revealed the potential regulatory roles of miRNAs in the immune response of these chicken lines against HPAI H5N1 virus infection in the lungs.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2023)
Article
Veterinary Sciences
Suyeon Kang, Thi Hao Vu, Jubi Heo, Chaeeun Kim, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study investigated the role of microRNAs (miRNAs) in immune responses against H5N1 infection and the development of disease-resistant chickens. The researchers characterized the miRNA expression patterns in tracheal tissues of H5N1-infected Ri chickens and found several miRNAs associated with immune-related genes. Network analysis revealed that these miRNAs may regulate gene signaling pathways related to influenza A virus progression.
JOURNAL OF VETERINARY SCIENCE
(2023)
Article
Fisheries
Thi Hao Vu, Jubi Heo, Suyeon Kang, Chaeeun Kim, Hyun S. Lillehoj, Yeong Ho Hong
Summary: This study investigates the role and mechanism of the chicken miRNA gga-miR-26a-5p in the immune response to HPAIV H5N1 infection. The results show that gga-miR-26a-5p acts as a translational repressor of MDA5 and negatively regulates the expression of signaling molecules and proinflammatory cytokines in the MDA5 signaling pathway. These findings contribute to a better understanding of the biological functions of gga-miR-26a-5p and the antiviral response in chickens.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jesper Tessin, Judith Rohde, Arne Jung, Nicole Kemper, Jochen Schulz
Summary: In this study, samples from poultry house environment and culled birds were collected, and E. cecorum was successfully isolated. The principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate transmission routes and optimize biosecurity measures.
Article
Agriculture, Dairy & Animal Science
Junqin Zhang, Zimin Xie, Yanlin Pan, Zuoxin Chen, Yunzhen Huang, Linlin Li, Jiawen Dong, Yong Xiang, Qi Zhai, Minhua Sun, Xingying Li, Shujian Huang, Ming Liao
Summary: In recent years, there has been an increase in the occurrence of fowl adenovirus 2 (FAdV-2) in China, posing a significant threat to the poultry industry. This study investigated the epidemiology, phylogenetic relationship, genomic characteristics, and pathogenicity of FAdV-2. The results showed that FAdV-2 was the most commonly detected serotype among various FAdV serotypes. Genetic analysis revealed that these FAdV-2 strains formed a distinct branch within the FAdV-D group and had a high nucleotide similarity with reference strains. Recombination analysis identified recombination events between FAdV-2 and FAdV-11 in some strains. Pathogenicity experiments demonstrated that a recombinant FAdV-2 strain caused a high mortality rate and more severe disease in chickens. These findings contribute to our understanding of FAdV-2 and provide a basis for vaccine development.
Article
Agriculture, Dairy & Animal Science
Ebtsam Iraqi, Ali Abdel Hady, Nadia Elsayed, Hanaa Khalil, Amina El-Saadany, Karim El-Sabrout
Summary: Thermal manipulation during egg incubation, particularly at 12 to 18 days, has positive effects on embryonic development, hatching characteristics, chick quality, as well as posthatch thermotolerance and performance.
Article
Agriculture, Dairy & Animal Science
Yong Liu, Mingxin Song, He Bai, Chunhua Wang, Fei Wang, Qi Yuan
Summary: Dietary curcumin supplementation can significantly improve egg quality of quails in the late laying period, primarily by improving lipid metabolism and selectively regulating the intestinal microbial community.
Article
Agriculture, Dairy & Animal Science
Lei Zhang, Zhixuan Song, Peiyao Li, Xingju Song, Xinming Tang, Dandan Hu
Summary: This study evaluated the vaccine potential of recombinant proteins for avian coccidiosis caused by Eimeria. The results showed that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
Article
Agriculture, Dairy & Animal Science
Milan Kumar Sharma, Amit Kumar Singh, Doyun Goo, Venkata Sesha Reddy Choppa, Hanseo Ko, Hanyi Shi, Woo Kyun Kim
Summary: The study investigates the effect of Eimeria infection on gastrointestinal physiology, growth performance, and egg production of Hy-Line W-36 laying hens during peak production. Results show that Eimeria infection negatively affects body weight, feed intake, immune response, and nutrient transporter gene expression. Egg production temporarily ceases in hens infected with high and medium-high dosages of Eimeria.
Article
Agriculture, Dairy & Animal Science
Elwy A. Ashour, Mahmoud Kamal, Hayman A. A. Altaie, Ayman A. Swelum, Gamaleldin M. Suliman, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack
Summary: This study evaluated the effects of CP and ME on the performance and nutritional value of Japanese quail lay chicken. The results showed that protein and energy levels significantly influenced all productive outcomes except feed conversion ratio. The combination of dietary energy and protein levels had a substantial effect on all egg quality trials evaluated. It is recommended to feed layer Japanese quail between the ages of 8 and 20 weeks during the summer with a dietary energy content of 2,900 kcal ME/kg and 20% CP.
Article
Agriculture, Dairy & Animal Science
J. E. Doornweerd, R. F. Veerkamp, B. de Klerk, M. van der Sluis, A. C. Bouwman, E. D. Ellen, G. Kootstra
Summary: Tracking group-housed individual broilers using video can provide valuable information on their health, welfare, and performance. This study conducted a comprehensive analysis on broiler tracking on video and potential tracking errors, highlighting the potential and challenges of phenotyping broiler locomotion. The results emphasized the importance of addressing tracking errors, potential algorithm improvements, and the need for an external animal identification system for efficient locomotion phenotyping.
Article
Agriculture, Dairy & Animal Science
Jiayu Wu, Yanan Wang, Yu An, Changyu Tian, Lingfeng Wang, Zuhong Liu, Desheng Qi
Summary: This study compared the gene expression differences in liver tissues of laying hens at different ages using RNA sequencing, and found that the transport and metabolism of amino acids changed during the growing period, which influenced the growth and development of the hens.
Article
Agriculture, Dairy & Animal Science
J. Raabe, G. Raveendran, W. Otten, K. Homeyer, T. Bartels
Summary: This study investigated the effects of different flicker frequencies on the behavior, performance, and stress response in male turkeys. The results showed that flicker frequencies of 165 Hz or higher had no detrimental effect on growth performance, injurious pecking, or endocrine stress response in the turkeys.
Article
Agriculture, Dairy & Animal Science
Adamu Mani Isa, Yanyan Sun, Yuanmei Wang, Yunlei Li, Jingwei Yuan, Aixin Ni, Hui Ma, Lei Shi, Hailai Hagos Tesfay, Yunhe Zong, Panlin Wang, Pingzhuang Ge, Jilan Chen
Summary: This study sequenced mRNAs and lncRNAs in the ovary stroma of specific chicken breeds and their reciprocal crossbreds to understand the molecular mechanism of heterosis for egg production. Results showed that genes with nonadditive expression were related to follicle growth and atresia as well as oxidative stress in the crossbred hens.
Article
Agriculture, Dairy & Animal Science
Zhepeng Wang, Qiu Chen, Yiwei Wang, Yulu Wang, Ruifang Liu
Summary: The study investigates the functional variants associated with chicken eggshell color in the SLCO1B3 gene by measuring the eggshell color of Lueyang black-boned chickens and analyzing the genetic data. It identifies 15 variants in SLCO1B3 that are significantly associated with eggshell color. However, the functional analysis shows that one specific variant, 1B3_SNP108, does not have a significant effect on promoter activity. This study highlights the importance of SLCO1B3 and its variants in breeding eggshell color.
Article
Agriculture, Dairy & Animal Science
P. Yin, Q. Tong, B. M. Li, W. C. Zheng, Y. Wang, H. Q. Peng, X. L. Xue, S. Q. Wei
Summary: This study investigated the welfare and health of laying hens in a multitier system. The results showed that the spatial distribution of hens changed with increasing age, and lower-ranking hens preferred to stay in lower tiers to avoid competition but still had access to resources. This improved the welfare and health of the hens near the end of the laying cycle.
Article
Agriculture, Dairy & Animal Science
Wenwen Liang, Siqiong Jie, Yiqin Wang, Xiaowei Wen, Yanling Xiong, Xiaoxi Lin, Zhengrong Lv
Summary: This study examined the effect of risk perceptions on preventive behavior among vendors in the live poultry wholesale market and the live poultry retail market in Guangdong Province using the health belief model and structural equation modeling. The results showed that perceived severity and perceived benefits positively influenced vendors' self-efficacy in the wholesale market, while perceived barriers negatively affected self-efficacy in both the wholesale and retail markets. Moreover, cues to action mediated the relationship between perceived severity, perceived benefits, and self-efficacy, and also mediated the effect of perceived susceptibility among wholesale market vendors.
Article
Agriculture, Dairy & Animal Science
Shreeya Sharma, Raveendra R. Kulkarni, Shayan Sharif, Hosni Hassan, Mohammadali Alizadeh, Scott Pratt, Khaled Abdelaziz
Summary: The study investigates the impact of exposing chick embryos to antigens via in ovo delivery of poultry-specific lactobacilli on the expression of genes associated with early bursal development and maturation. Results indicate that the supplementation of poultry-specific lactobacilli to chick embryos can accelerate the development and immunological maturation of the bursa of Fabricius.