期刊
NEURON
卷 110, 期 22, 页码 3727-+出版社
CELL PRESS
DOI: 10.1016/j.neuron.2022.08.015
关键词
-
资金
- NIH [R01NS089787]
- American Cancer Society [122229-IRG-97-153-10-IRG]
- NSF [CMMI 1761432]
This study investigates nocifensive behavior in Drosophila larvae and reveals the critical role of shear stress in activating nociceptors. The study also identifies TRPA1 as a mechanosensitive channel specifically activated by shear stress.
Mechanical nociception is essential for animal survival. However, the forces involved in nociceptor activation and the underlying mechanotransduction mechanisms remain elusive. Here, we address these problems by investigating nocifensive behavior in Drosophila larvae. We show that strong poking stimulates nociceptors with a mixture of forces including shear stress and stretch. Unexpectedly, nociceptors are selectively activated by shear stress, but not stretch. Both the shear stress responses of nociceptors and nocifensive behavior require transient receptor potential A1 (TrpA1), which is specifically expressed in nociceptors. We further demonstrate that expression of mammalian or Drosophila TrpA1 in heterologous cells confers responses to shear stress but not stretch. Finally, shear stress activates TrpA1 in a membrane-delimited manner, through modulation of membrane fluidity. Together, our study reveals TrpA1 as an evolutionarily conserved mechanosensitive channel specifically activated by shear stress and suggests a critical role of shear stress in activating nociceptors to drive mechanical nociception.
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