期刊
NATURE METHODS
卷 19, 期 9, 页码 1072-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-022-01577-1
关键词
-
资金
- Max Planck Society
The combination of MINFLUX nanoscopy with DNA-PAINT labeling enables multi-color imaging of multiple molecular targets, leading to improved nanoscopy in fixed cells and increased multiplexing in MINFLUX imaging, as demonstrated by three-color imaging of mitochondria in mammalian cells.
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on-off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells. A systematic exploration of MINFLUX nanoscopy with DNA-PAINT labeling leads to improved nanoscopy in fixed cells and MINFLUX imaging with increased multiplexing, as exemplified by three-color imaging of mitochondria in mammalian cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据