Article
Biochemistry & Molecular Biology
Shubhi Pandey, Punita Kumari, Mithu Baidya, Ryoji Kise, Yubo Cao, Hemlata Dwivedi-Agnihotri, Ramanuj Banerjee, Xaria X. Li, Cedric S. Cui, John D. Lee, Kouki Kawakami, Jagannath Maharana, Ashutosh Ranjan, Madhu Chaturvedi, Gagan Deep Jhingan, Stephane A. Laporte, Trent M. Woodruff, Asuka Inoue, Arun K. Shukla
Summary: The study uncovers that non-canonical 7TMRs, D6R and C5aR2, efficiently couple to parrs with distinct engagement of GRKs and activation of non-canonical downstream signaling pathways.
Review
Cell Biology
Gennaro Napolitano, Chiara Di Malta, Andrea Ballabio
Summary: The mTORC1 signaling pathway integrates multiple environmental cues to regulate cell growth and metabolism. Recent studies have revealed a 'non-canonical' signaling pathway of mTORC1 that controls the function of MiT-TFE transcription factors, key regulators of cell metabolism, and responds to stimuli that converge on the lysosomal surface.
TRENDS IN CELL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Mengjiao Shi, Jian Zhang, Miaomiao Li, Yaping Zhao, Ying Guo, Jiayi Xu, Rongrong Liu, Zongfang Li, Dongmei Ren, Pengfei Liu
Summary: This study investigated the protective effects of liquiritigenin (LQ), a natural flavonoid, against oxidative hepatotoxicity and the underlying mechanism. It was found that LQ eliminated reactive oxygen species in liver cells through NRF2 activation, involving both canonical and non-canonical signaling pathways. LQ-induced NRF2 activation was partially dependent on KEAP1-C151 and was also mediated by the blockage of autophagic flux and upregulation of p62, which competitively bound with KEAP1. Overall, this study reveals a novel molecular mechanism underlying the hepatoprotection of LQ, providing new insights into the pathogenesis and therapeutic strategies for oxidative liver injury.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Andrew B. Kleist, Shawn Jenjak, Andrija Sente, Lauren J. Laskowski, Martyna Szpakowska, Maggie M. Calkins, Emilie Anderson, Lisa M. McNally, Raimond Heukers, Vladimir Bobkov, Francis C. Peterson, Monica A. Thomas, Andy Chevigne, Martine J. Smit, John D. McCorvy, M. Madan Babu, Brian F. Volkman
Summary: NMR experiments on ACKR3 revealed that the recruitment of beta-arrestins is associated with conformational exchange at key regions of the receptor, and identified an allosteric hub in the core receptor that coordinates transitions among different conformational states, guiding beta-arrestin recruitment.
Article
Pharmacology & Pharmacy
Yujin Wang, Xiaoyan Shen, Qingwen Wang, Zixin Guo, Liwen Hu, Zhe Dong, Weidong Hu
Summary: The study found that the levels of RBJ protein were increased in tumor tissue of non-small cell lung cancer (NSCLC) and correlated positively with clinical stage. Silencing RBJ inhibited the growth, invasion, and migration of NSCLC cells, while overexpression of RBJ accelerated tumor formation. The results suggest that RBJ promotes the progression of NSCLC by activating the epithelial-mesenchymal transition (EMT) signaling pathway.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Cell Biology
Tara Akhshi, William S. Trimble
Summary: The study reveals that Smo induces ciliogenesis through two distinct pathways while also activating autophagy, with the Gα(i)-LGN-NuMA-dynein complex playing a crucial role in the process. Different Smo agonists activate these pathways separately, and only together they can replicate the activity of Hh ligand.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Cell Biology
Laurence Langlois-Lemay, Damien D'Amours
Summary: Centrosomes have diverse roles in addition to their well-known function as microtubule organizing centers in eukaryotic cells. They act as important structural platforms, together with their functional counterparts in lower eukaryotes, the spindle pole bodies, orchestrating signaling events essential for cell cycle progression, cellular responses to DNA damage, sensory reception, and cell homeostasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Karla Rubio, Addi J. Romero-Olmedo, Pouya Sarvari, Guruprasadh Swaminathan, Vikas P. Ranvir, Diana G. Rogel-Ayala, Julio Cordero, Stefan Guenther, Aditi Mehta, Birgit Bassaly, Peter Braubach, Malgorzata Wygrecka, Stefan Gattenloehner, Achim Tresch, Thomas Braun, Gergana Dobreva, Miguel N. Rivera, Indrabahadur Singh, Johannes Graumann, Guillermo Barreto
Summary: A study revealed that non-canonical ITGB2 signaling activates EGFR and RAS/MAPK/ERK signaling in small cell lung cancer (SCLC), providing a potential target for therapy independent of EGFR mutations.
Article
Biochemistry & Molecular Biology
Zuoning Han, Yanling Ma, Gary Cao, Zhengping Ma, Ruihua Chen, Mary Ellen Cvijic, Dong Cheng
Summary: The study found that αV01 integrin plays a role in non-canonical TGF-0 signaling pathways and that αV01 inhibition may be an effective strategy for the treatment of liver fibrosis.
BIOCHEMICAL JOURNAL
(2021)
Article
Cell Biology
Bartika Ghoshal, Edouard Bertrand, Suvendra N. Bhattacharyya
Summary: This study demonstrates that EV-delivered miR-122 is primarily single-stranded and is loaded onto recipient cell argonaute proteins to become functional. Following endocytosis, EV-associated miR-122 is released on the endosomal membrane in a pH-dependent manner, facilitating the formation of the exogenous miRNP pool in recipient cells.
JOURNAL OF CELL SCIENCE
(2021)
Review
Multidisciplinary Sciences
Theo Accogli, Christophe Hibos, Frederique Vegran
Summary: This article reviews the canonical and non-canonical functions of NLRP3 and their impact on various pathologies. Studying the functions of NLRP3 can provide a better understanding of its role in different diseases and help identify new therapeutic strategies.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Cell Biology
Mukulika Bose, Priyanka Grover, Alexa J. Sanders, Ru Zhou, Mohammad Ahmad, Sophia Shwartz, Priyanka Lala, Sritama Nath, Mahboubeh Yazdanifar, Cory Brouwer, Pinku Mukherjee
Summary: This study finds that MUC1 plays a significant role in the development of pancreatic ductal adenocarcinoma (PDA). Overexpression of MUC1 activates the JNK pathway and increases cell viability. Conversely, low expression of MUC1 allows TGF-beta to retain its tumor-suppressive function. These findings contribute to a better understanding of the mechanisms behind PDA development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Neil D. Perkins
Summary: Numerous studies have shown the key role of I kappa B kinase (IKK) subunits, alpha and beta, in regulating the Nuclear Factor kappa B (NF-kappa B) pathway. While previous research suggested separate functions for IKK beta and IKK alpha, new findings demonstrate that IKK alpha is an important regulator of the canonical NF-kappa B pathway in KRAS driven colorectal cancer cell lines.
BIOCHEMICAL JOURNAL
(2022)
Review
Cell Biology
Lukas Busch, Simone Eggert, Kristina Endres, Bernd Bufe
Summary: Recent research has focused on the pro-inflammatory activity of amyloid beta (Aβ) on microglia cells in Alzheimer's Disease (AD). There are over 100 different Aβ variants with variable length and chemical modifications, and their biological significance for AD is not fully understood, except for Aβ(1-42) and Aβ(1-40). This study aims to provide a comprehensive overview of the contributions of these neglected Aβ variants to microglia activation.
Article
Biochemistry & Molecular Biology
Chuntian Huang, Ruijuan Du, Xuechao Jia, Kangdong Liu, Yan Qiao, Qiong Wu, Ning Yao, Lu Yang, Liting Zhou, Xuejiao Liu, Pu Xiang, Mingxia Xin, Yan Wang, Xiaojie Chen, Dong Joon Kim, Zigang Dong, Xiang Li
Summary: The study revealed that CDK15 plays a pivotal role in colorectal cancer progression by phosphorylating PAK4 to promote tumor growth. The CDK15-PAK4 axis may serve as a novel therapeutic target for colorectal cancer.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Chemistry, Multidisciplinary
Do-Hyeon Kim, Kai Zhou, Dong-Kyun Kim, Soyeon Park, Jungeun Noh, Yonghoon Kwon, Dayea Kim, Nam Woong Song, Jong-Bong Lee, Pann-Ghill Suh, Nam Ki Lee, Sung Ho Ryu
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Article
Cell Biology
Parkyong Song, Yonghoon Kwon, Kyungmoo Yea, Hyo-Youl Moon, Jong Hyuk Yoon, Jaewang Ghim, Hyunjung Hyun, Dayea Kim, Ara Koh, Per-Olof Berggren, Pann-Ghill Suh, Sung Ho Ryu
CELLULAR SIGNALLING
(2015)
Article
Biochemical Research Methods
Jong Hyuk Yoon, Dayea Kim, Jin-Hyeok Jang, Jaewang Ghim, Soyeon Park, Parkyong Song, Yonghoon Kwon, Jaeyoon Kim, Daehee Hwang, Yoe-Sik Bae, Pann-Ghill Suh, Per-Olof Berggren, Sung Ho Ryu
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Multidisciplinary Sciences
Youngwoo Choi, Yonghoon Kwon, Dae-Kyum Kim, Jinseong Jeon, Su Chul Jang, Taejun Wang, Minjee Ban, Min-Hye Kim, Seong Gyu Jeon, Min-Sun Kim, Cheol Soo Choi, Young-Koo Jee, Yong Song Gho, Sung Ho Ryu, Yoon-Keun Kim
SCIENTIFIC REPORTS
(2015)
Article
Chemistry, Multidisciplinary
Do-Hyeon Kim, Dong-Kyun Kim, Kai Zhou, Soyeon Park, Yonghoon Kwon, Min Gyu Jeong, Nam Ki Lee, Sung Ho Ryu
Article
Biochemistry & Molecular Biology
Do-Hyeon Kim, Soyeon Park, Dong-Kyun Kim, Min Gyu Jeong, Jungeun Noh, Yonghoon Kwon, Kai Zhou, Nam Ki Lee, Sung Ho Ryu
Review
Biochemistry & Molecular Biology
Parkyong Song, Yonghoon Kwon, Jae-Yeol Joo, Do-Geun Kim, Jong Hyuk Yoon
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Min Gyu Jeong, Kai Zhou, Soyeon Park, HyeongJeon An, Yonghoon Kwon, Yeonho Chang, Do-Hyeon Kim, Sung Ho Ryu
Summary: A new method for analyzing protein interactions in the outer membrane of living cells has been developed, which could provide critical information to understand how cancer cells develop resistance to targeted drug therapies. By investigating weak protein-protein interactions, researchers found that the cancer-linked protein EGFR tends to bind with a range of other proteins, depending on the type of cancer cell studied, potentially contributing to drug resistance.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yeonho Chang, Do-Hyeon Kim, Kai Zhou, Min Gyu Jeong, Soyeon Park, Yonghoon Kwon, Triet Minh Hong, Jungeun Noh, Sung Ho Ryu
Summary: Researchers have developed a new microscopy method called QD-PAINT, which shows promising potential to overcome the limited resolution of current SMLM by utilizing quantum dots (QDs) with improved photoswitchability and specificity. By adapting QDs for SMLM, researchers achieved an improved resolution of around 8 nm, significantly enhancing the spatial resolution compared to existing methods using organic dyes. This novel approach could potentially lead to even higher resolutions in the future.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2021)
Article
Medicine, Research & Experimental
Parkyong Song, Han-Seul Jo, Wan-Seog Shim, Yang Woo Kwon, Sungwon Bae, Yonghoon Kwon, Bakhovuddin Azamov, Jin Hur, Dongjun Lee, Sung Ho Ryu, Jong Hyuk Yoon
Summary: Emodin promotes type I collagen synthesis in dermal fibroblasts, potentially serving as a candidate for treating skin aging and wrinkles.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yonghoon Kwon, Do-Hyeon Kim, Min Gyu Jeong, Minh-Triet Hong, Soyeon Park, Yeonho Chang, Kai Zhou, Seung-Yeol Park, Jin Zhang, Sung Ho Ryu
Summary: This study reveals the importance of dimerization of the β2-adrenergic receptor for generating inverse agonism and identifies cholesterol as a specific regulator in this process.
CELL CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Brian Tenner, Jason Z. Zhang, Yonghoon Kwon, Veronica Pessino, Siyu Feng, Bo Huang, Sohum Mehta, Jin Zhang
Summary: FluoSTEPs is a novel class of biosensors that combines self-complementing split green fluorescent protein, CRISPR-mediated knock-in, and fluorescence resonance energy transfer technology to probe compartmentalized signaling dynamics in situ. It can simultaneously highlight endogenous microdomains and report domain-specific, real-time signaling events, including kinase activities, guanosine triphosphatase activation, and second messenger dynamics. FluoSTEPs have been shown to be useful in probing spatiotemporal regulation within endogenous signaling architectures, as demonstrated by a FluoSTEP for 3',5'-cyclic adenosine monophosphate (cAMP) revealing distinct cAMP dynamics within clathrin microdomains in response to stimulation of G protein-coupled receptors.
Article
Chemistry, Multidisciplinary
Do-Hyeon Kim, Yeonho Chang, Soyeon Park, Min Gyu Jeong, Yonghoon Kwon, Kai Zhou, Jungeun Noh, Yun-Kyu Choi, Triet Minh Hong, Young-Tae Chang, Sung Ho Ryu
Summary: Multicolor fluorescence imaging is a powerful tool for visualizing the spatiotemporal relationship among biomolecules. However, commonly employed organic dyes exhibit a blue-conversion phenomenon which can lead to severe multicolor image artifacts, particularly in localization-based superresolution microscopy. Therefore, practical guidelines for the use of organic dyes in multicolor imaging have been provided to prevent artifacts caused by blue-conversion.
Article
Biochemistry & Molecular Biology
Chaithanya Chelakkot, Youngwoo Choi, Dae-Kyum Kim, Hyun T. Park, Jaewang Ghim, Yonghoon Kwon, Jinseong Jeon, Min-Seon Kim, Young-Koo Jee, Yong S. Gho, Hae-Sim Park, Yoon-Keun Kim, Sung H. Ryu
EXPERIMENTAL AND MOLECULAR MEDICINE
(2018)