Article
Biochemical Research Methods
Pan Deng, Taylor Valentino, Michael D. Flythe, Hunter N. B. Moseley, Jacqueline R. Leachman, Andrew J. Morris, Bernhard Hennig
Summary: By studying metabolites produced by the gut microbiome, differences in the utilization of different dietary fibers for biosynthesis of essential molecules were observed, providing a new method for a deeper understanding of the metabolic function of the gut microbiome.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Richard C. Burrell, Samuel J. Bonacorsi, Adrian Ortiz, Tamas Benkovics, Zhongping Shi
Summary: Censavudine, a drug used for treating HIV-1, has been synthesized with carbon-14 and deuterium labels for research purposes. The synthesized analogs have high purity and meet the requirements for clinical studies.
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2022)
Article
Agriculture, Multidisciplinary
Ryo Nakabayashi, Yutaka Yamada, Tomoko Nishizawa, Tetsuya Mori, Takashi Asano, Masanari Kuwabara, Kazuki Saito
Summary: The biosynthetic pathway of the ACE inhibitor asparaptine in asparagus was investigated, revealing new metabolites and analogues. Oral administration of asparaptine A to hypertensive mice showed a blood pressure-lowering effect within 2 hours, suggesting potential therapeutic effects beyond ACE inhibition.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Environmental Sciences
Maaike Goudriaan, Victor Hernando Morales, Marcel T. J. van der Meer, Anchelique Mets, Rachel T. Ndhlovu, Johan van Heerwaarden, Sina Simon, Verena B. Heuer, Kai-Uwe Hinrichs, Helge Niemann
Summary: We developed an assay using stable isotope tracer techniques to determine microbial plastic mineralization rates in liquid medium on a lab scale. By incubating C-13-labeled polyethylene particles with Rhodococcus ruber as a model organism, we found that microbially mediated mineralization rates of up to 1.2% yr(-1) of the added plastic could be achieved. Isotope tracer techniques were also able to detect C-13 enrichment in membrane fatty acids of R. ruber after incubation, indicating microbial plastic degradation.
MARINE POLLUTION BULLETIN
(2023)
Article
Medicine, Research & Experimental
Matthew D. Taves, Shizuka Otsuka, Michaela A. Taylor, Kaitlynn M. Donahue, Thomas J. Meyer, Margaret C. Cam, Jonathan D. Ashwell
Summary: Glucocorticoids, produced by the enzyme 1113-hydroxysteroid dehydrogenase type 1 (1113-HSD1), play a role in tumor progression by suppressing the immune response. Inhibition of 1113-HSD1 reduces tumor growth and increases sensitivity to immunotherapy. Targeting 1113-HSD1 may be beneficial in cancer therapy.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Yongjie Zhang, Jie Xu, Jie Zhao, Huili Chen, Ning Li, Xijing Chen, Di Zhao
Summary: This study used ICP-MS and stable isotope labeling strategy to systematically investigate the pharmacokinetic properties of Hemin. The results showed that Hemin was rapidly absorbed in the gastrointestinal tract and distributed to various organs, with predominant excretion via feces. The majority of Hemin in plasma was found to be bound to proteins.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2023)
Article
Chemistry, Analytical
Chien-Yu Su, Jie-Huei Wang, Tien-Yi Chang, Chia-Lung Shih
Summary: A two-stage data-processing approach combining MDF and SIT was developed to improve the efficacy of drug metabolite identification. The new approach significantly increased the validated rate and discovered several novel metabolites related to the toxicity of PIO.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemistry & Molecular Biology
Doaa A. Osman, Mario A. Macias, Lamya H. Al-Wahaibi, Nora H. Al-Shaalan, Luke S. Zondagh, Jacques Joubert, Santiago Garcia-Granda, Ali A. El-Emam
Summary: The solid-state structural analysis and docking studies of three adamantane-linked 1,2,4-triazole derivatives show that while electronic nature of the groups does not influence solid formation, there may be a correlation with group size. Despite similarities in intermolecular disposition, pairwise interaction energies vary between the compounds, influencing total packing energy. HOMO-LUMO energies indicate influence of NO2 group on reactivity, suggesting potential for inhibiting 11 beta-HSD1 enzyme.
Article
Chemistry, Organic
Yuzhao Hu, Haiyuan Quan, Liuyang Wang, Zhinan Wang, Xiangdong Mei, Jun Nin, Dongmei She
Summary: This article described a method for synthesizing C-13(4)-1,4-dihydropyridines with simple operation, mild reaction conditions, and high yield. This method provides a new idea for introducing 1,4-dihydropyridines labeled with C-13 isotope, meeting the requirements for independent synthesis of stable isotope internal standards for consistency evaluation of generic drugs in China.
CHINESE JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Environmental Sciences
Huanjun Zhang, Yin Lu, Yi Li, Lei Wang, Wenlong Zhang, Longfei Wang, Lihua Niu, Zhongjun Jia
Summary: This study identified bacteria involved in estrogen metabolism in sediments from different bays of Taihu Lake using DNA-SIP technology, revealing their distinct roles in the biodegradation process of E2. The results showed a significant and positive correlation between the increase in relative abundance of these bacteria and E2 mineralization rate.
ENVIRONMENTAL POLLUTION
(2021)
Article
Toxicology
Anne Landmesser, Max Scherer, Gerhard Scherer, Mohamadi Sarkar, Jeffery S. Edmiston, Reinhard Niessner, Nikola Pluym
Summary: The study suggests that while e-cigarettes produce toxic carbonyls and epoxides, the overall exposure to these toxicants from vaping is relatively low compared to other sources. This indicates that vaping is a minor contributor to users' exposure to these harmful substances.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemical Research Methods
Valentin Goeldner, Jens Fangmeyer, Uwe Karst
Summary: This study presents a cheap and straightforward method for synthesizing milligram amounts of isotopically labeled metabolite standards, addressing issues like electrode fouling and cross contamination with exchangeable working electrodes. The developed approach was successfully applied to the isotopically labeled synthesis of acetaminophen, demonstrating its potential for the synthesis of metabolite standards.
DRUG TESTING AND ANALYSIS
(2022)
Article
Biochemical Research Methods
Jixia Yang, Gongzheng Zhang, Zhaoyang Wang, Jian Meng, Hongliang Wen
Summary: This study discusses a novel metabolic pathway for an indolinone derivative, demonstrating its good metabolic stability. The metabolite analysis revealed key information for developing effective alpha-syn aggregate inhibitors and PET-tracer-related indolinone derivatives.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Chemistry, Organic
Shilong Zheng, Jiawang Liu, Borui Kang, Guangdi Wang
Summary: ZB716-d6, a deuterated form of ZB716, was successfully synthesized in eight steps from a commercially available deuterium-labeled starting material and has been utilized as an internal standard in clinical bioanalysis with good yield and high purity.
SYNTHETIC COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Shabbir Ahmad, Mujahid Mohammed, Lakshmi Prasuna Mekala, Rai Anusha, Chintalapati Sasikala, Chintalapati Venkata Ramana
Summary: Anoxygenic photosynthetic bacteria have metabolic versatility and can survive with a wide range of carbon and nitrogen sources. Rubrivivax benzoatilyticus JA2, a member of this group, can utilize L-tryptophan as a nitrogen source and produce indolic compounds with biotechnological significance. Studying the chemotrophic L-tryptophan metabolism of strain JA2 provides new insights into its metabolic potential.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
