Article
Biochemistry & Molecular Biology
Lei Peng, Yijing He, Weihan Wang, Yajie Chu, Qixin Lin, Rong Rui, Qiao Li, Shiqiang Ju
Summary: In this study, the importance of PAK1 in regulating spindle assembly and cell cycle progression during the first meiotic division of porcine oocytes was highlighted. The activated PAK1 showed dynamic expression and its subcellular localization was closely associated with spindle dynamics. Inhibition of PAK1 activity by IPA-3 led to impaired extrusion of PB1 and failure of bipolar spindle formation, which could be restored by reducing disulfide bonds between PAK1 and IPA-3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Cong Ma, Zhuonan Yang, Shouxin Zhang, Xueke Zhang, Siyuan Wang, Huiru Cheng, Yang Liu, Hongzhen Ruan, Zuying Xu, Chunmei Liang, Dan Liang, Zhiming Ding, Yajing Liu, Yunxia Cao
Summary: CBZ exposure negatively affects the maturation of mouse oocytes, leading to disruptions in polar body extrusion, cell cycle distribution, spindle assembly, chromosomal alignment, and kinetochore microtubule attachment.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Feng-Xin Qiao, Ming-Xin Sun, Zhi-Ran Xu, Yue-Cen Liu, Yan-Zhu Chen, Hai-Long Wang, Zhong-Quan Qi, Chang-Long Xu, Yu Liu
Summary: This study found that exposure to chloroacetonitrile (CAN) significantly impairs the maturation of mouse oocytes, alters the expression of multiple oocyte genes, induces reactive oxygen species production, ER stress and affects spindle assembly. In vivo experiments also showed impaired follicular development. These findings suggest that CAN exposure has adverse effects on mouse oocytes.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiyeon Leem, Jeong Su Oh
Summary: In this study, researchers discovered that MDC1 plays a non-canonical role in controlling G2/M transition in mouse oocytes by regulating APC/C-Cdh1-mediated cyclin B1 degradation in response to DNA damage. They also found that MDC1 depletion impairs spindle assembly by decreasing the integrity of microtubule organizing centers (MTOCs). These findings provide new insights into the regulation of the G2/M DNA damage checkpoint and cell cycle control in oocytes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Umut Toprak, Cansu Dogan, Dwayne Hegedus
Summary: This review focuses on the role of two Ca2+ channel receptors in insect cells, IP3R and RyR, which share similarities in structure and function but differ in phylogenetic origins. Both receptors have great potential as target sites in pest control, with RyRs currently being targeted by commercial insecticides.
Article
Biochemistry & Molecular Biology
HaiYan Wang, Jinrong Hu, Kexi Yi, Zhiming Ma, XinJie Song, Yaelim Lee, Petr Kalab, Alexander D. Bershadsky, Yansong Miao, Rong Li
Summary: In this study, the recruitment of FMN2 to the ER around the spindle and its activity regulation in mouse meiosis I oocytes were investigated. The results showed that this process is regulated by the Ran GTPase and the ER-associated protein VAPA. The binding of FMN2 to importin a1/b complex and its subsequent disruption by RanGTP were required for FMN2 accumulation near the chromatin and the MI spindle. The importin-free FMN2 was then recruited to the ER surface around the spindle through its interaction with VAPA. Moreover, autoinhibition of FMN2 through an intramolecular interaction between its SLD and FH2 domain was relieved by the binding of VAPA, leading to localized actin polymerization. This study sheds light on the crucial role of FMN2 in the maturation of mammalian female gametes.
Article
Multidisciplinary Sciences
Vikas Arige, Lara E. Terry, Larry E. Wagner, Sundeep Malik, Mariah R. Baker, Guizhen Fan, Suresh K. Joseph, Irina I. Serysheva, David I. Yule
Summary: Inositol 1,4,5-trisphosphate receptors (IP3Rs) initiate physiological responses by regulating intracellular calcium signals. This study identifies Ca2+ binding sites and acidic residues that regulate IP3Rs channel activity, providing insights into their regulatory mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Ana M. Rossi, Andrew M. Riley, Genevieve Dupont, Taufiq Rahman, Barry V. L. Potter, Colin W. Taylor
Summary: Inositol 1,4,5-trisphosphate receptors (IP(3)Rs) are intracellular Ca2+ channels linking extracellular stimuli to Ca2+ signals. Research shows that quantal responses to low IP3 concentrations do not require heterogeneous Ca2+ stores, and IP(3)Rs exhibit incremental responses to IP3 stimulation. This suggests that Ca2+ signals evoked by IP3 pulses arise from rapid activation and inactivation of a small fraction of IP(3)Rs.
Article
Cell Biology
Giacomo Dentoni, Luana Naia, Maria Ankarcrona
Summary: Mitochondria-endoplasmic reticulum contact sites (MERCS) are a subcellular region that plays a role in various physiological and pathological mechanisms. By studying neuroblastoma cells, it has been found that MERCS can affect exocytosis and specific regulatory mechanisms have been identified.
Article
Multidisciplinary Sciences
Nagendra Babu Thillaiappan, Holly A. Smith, Peace Atakpa-Adaji, Colin W. Taylor
Summary: The study demonstrates that the KRas-induced KRAP protein tethers a subset of IP3 receptors to actin, enabling them to evoke calcium signals. Loss of KRAP results in the disappearance of Ca2+ puffs and a global response to increased cytosolic Ca2+ concentration from more intense stimulation.
NATURE COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Jiyeon Leem, Seul Kim, Jae-Sung Kim, Jeong Su Oh
Summary: In this study, the effects of exposure to 9,10-phenanthrenequinone (9,10-PQ) were investigated using mouse oocytes as a model system. It was found that 9,10-PQ compromised meiotic maturation by impairing the assembly of the microtubule organizing center and subsequent spindle formation. Additionally, 9,10-PQ exposure inhibited cell cycle progression by disrupting the accumulation of cyclin B1 and the activation of Cdk1. Importantly, the meiotic defects induced by 9,10-PQ were not rescued by decreasing reactive oxygen species (ROS) levels, indicating that 9,10-PQ has ROS-independent activity in regulating cell cycle progression and spindle assembly.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Immunology
Prarthana J. Dalal, David P. Sullivan, Evan W. Weber, David B. Sacks, Matthias Gunzer, Isabella M. Grumbach, Joan Heller Brown, William A. Muller
Summary: The research highlights the important roles of endothelial CaM and CaMKIIδ in transducing specific calcium signals required for TEM, which interferes with the delivery of the lateral border recycling compartment (LBRC) and reduces TEM.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Alexis Shiying Huang, Benjamin Chun -Kit Tong, Harry Chun-Hin Hung, Aston Jiaxi Wu, Olivia Ka-Yi Ho, Anna Hau-Yee Kong, Maggie Ming-Ki Leung, Jingxuan Bai, Xiuqiong Fu, Zhiling Yu, Min Li, Ting Fan Leung, Judith Choi-Wo Mak, George Pak-Heng Leung, King -Ho Cheung
Summary: Asthma is a chronic inflammatory disease characterized by airway hypersensitivity and remodeling. Houttuynia cordata, a traditional Chinese herb, may modulate intracellular Ca2+ signaling and be a potential alternative treatment choice for asthma. The upregulation of inositol trisphosphate receptors (IP3Rs) and enhanced intracellular Ca2+ release contribute to airway remodeling, while pretreatment with H. cordata essential oil rectifies Ca2+ signaling disruption and mitigates asthma development. Houttuynin/2-undecanone could be the bioactive component in H. cordata essential oil and directly inhibits IP3R expression.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cardiac & Cardiovascular Systems
Kazuki Kodo, Keiko Uchida, Hiroyuki Yamagishi
Summary: Congenital heart disease (CHD) is a common life-threatening anomaly caused by defects in cardiovascular development, with genetic and environmental factors playing a key role. Understanding the genes involved is crucial for uncovering the genomic architecture of CHD.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Plant Sciences
Yazhong Wang, Yan Wang, Jie Zang, Huabang Chen, Yan He
Summary: The function of PRD1 in regulating meiotic DSB formation is conserved in maize, but its role in bipolar spindle assembly shows divergence among different plant species. ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis, but the function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Cell Biology
X. Johne Liu
CELL AND TISSUE RESEARCH
(2016)
Article
Obstetrics & Gynecology
Yong Tao, Dandan Liu, Guolong Mo, Hongmei Wang, X. Johne Liu
HUMAN REPRODUCTION
(2015)
Article
Multidisciplinary Sciences
Yong Tao, Johne Liu
Article
Developmental Biology
Dandan Liu, Guolong Mo, Yong Tao, Hongmei Wang, X. Johne Liu
REPRODUCTION FERTILITY AND DEVELOPMENT
(2017)
Article
Cell Biology
Yong Tao, X. Johne Liu
Editorial Material
Cell Biology
X. Johne Liu, Yong Tao
Article
Reproductive Biology
Geoffrey G. Leblond, Heather Sarazin, Ruizhen Li, Makoto Suzuki, Naoto Ueno, X. Johne Liu
BIOLOGY OF REPRODUCTION
(2012)
News Item
Cell Biology
X. Johne Liu
Article
Cell Biology
Hua Shao, Chunqi Ma, Xuan Zhang, Ruizhen Li, Ann L. Miller, William M. Bement, X. Johne Liu
Article
Cell Biology
Dandan Liu, Hua Shao, Hongmei Wang, X. Johne Liu
Review
Cell Biology
X. Johne Liu
Article
Cell Biology
Hua Shao, Ruizhen Li, Chunqi Ma, Eric Chen, X. Johne Liu
JOURNAL OF CELL BIOLOGY
(2013)
Article
Developmental Biology
J. Leblanc, X. Zhang, D. McKee, Z. -B. Wang, R. Li, C. Ma, Q. -Y. Sun, X. J. Liu
MOLECULAR HUMAN REPRODUCTION
(2011)
Article
Cell Biology
Guolong Mo, Ruizhen Li, Zachary Swider, Julie Leblanc, William M. Bement, X. Johne Liu
Summary: A transient increase in calcium ions at the plasma membrane associated with the contractile ring promotes polar body abscission in Xenopus laevis oocytes.