期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 140, 期 -, 页码 39-41出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2016.08.012
关键词
Vertical transmission; OsHV-1; Crassostrea angulata; Offspring; Swim up
类别
资金
- BONAQUA project [0433_BONAQUA_5_E POCTEP]
- IFAPA - European Union FEDER program
- FCT [SFRH/BPD/108591/2015]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/108591/2015] Funding Source: FCT
In order to determine if ostreid herpesvirus 1 (OsHV-1) can be vertically transmitted, 9 full-sib families of the Portuguese oyster Crassostrea angulata were produced using a factorial mating design with 3 males and 3 females. The parents were survivors from an OsHV-1 mortality outbreak. OsHV-1 DNA was not detected by conventional PCR in the mantle of parents, gametes or 3 day-old larvae. However, viral DNA was detected by real-time PCR in all gametes and larvae samples. These results show that C. angulata that have survived an OsHV-1 mortality outbreak can carry the virus and vertically transmit it to their offspring. (C) 2016 Elsevier Inc. All rights reserved.
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