期刊
JOURNAL OF INVERTEBRATE PATHOLOGY
卷 134, 期 -, 页码 1-5出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2015.12.008
关键词
Honey bee; Specific primers; Lotmaria passim; PCR detection; Trypanosomatidae
类别
资金
- National Commission for Scientific and Technological Research, CONICYT, Chile [1140653, 3250231]
The recently described trypanosome Lotmaria passim is currently considered the most predominant trypanosomatid in honey bees worldwide and could be a factor in honey bee declines. For a specific and quick detection of this pathogen, we developed primers based on the SSU rRNA and gGAPDH genes for the detection of L. passim in Chilean honey beehives. PCR products amplified and sequenced for these primers shared 99-100% identity with other sequences of L. passim. The designed primers were specific and we were able to detect a high prevalence (40-90%) of L. passim in bee hives distributed throughout Chile. Our described PCR-based method offers a feasible and specific detection of L passim in any honey bee samples. (C) 2015 Elsevier Inc. All rights reserved.
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