期刊
CARBOHYDRATE POLYMERS
卷 117, 期 -, 页码 1021-1027出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.09.082
关键词
Chinese yam polysaccharide; Purification; Structural characterization; Antioxidative activity; Antibacterial activity
资金
- National Natural Science Foundation of China [31171658]
Purification and structural characterization of Chinese yam polysaccharide were investigated and its activities were analyzed. Results indicated that a single component polysaccharide with a molecular weight of 16,619 Da was obtained after hot water extraction with sequential sevage deproteinization, HSCCC and Sephadex G-100 size-exclusion chromatography. The FTIR analysis showed that it had characteristic absorptive peaks and contained uronic acid. The methylation and GC-MS analysis showed that it comprised of glucose and galactose with a molar ratio of 1.52:1, and that it mainly contained 1,3-linked-glc, 1-linked-gal and 1,6-linked-gal glycosidic bonds. H-1 NMR and C-13 NMR spectra analysis showed that there were two alpha-configurations and one beta-configuration, and that beta-1,3-glucose, alpha-1-galactose, alpha-1,6-galactose might exist in the structure of the purified polysaccharide. The determination of the antioxidative activity showed that it could scavenge hydroxyl and superoxide radicals. The purified polysaccharide displayed a certain inhibitory activity against Escherichia coli, with a MIC of 2.5 mg/mL. (C) 2014 Elsevier Ltd. All rights reserved.
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