期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 155, 期 -, 页码 105-114出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2015.11.025
关键词
Metallacrown; G-quadruplex destabilization; Ligand binding; FID assay; Tb(III) luminescence
资金
- National Science Centre of Poland [2014/13/N/ST4/04099]
Interactions of the G-quadruplex (GQ) DNA with two pentacoordinate lanthanide (III) metallacrown (MC) complexes containing phenylalanine hydroxamic acid (pheHA) and copper(II) ions of the formula Eu 15-[MCCu,pheHA]-5 (1) and Tb 15-[MCCu,pheHA]-5 (2) were investigated. Binding of both metallacrowns to human telomeric G-quadruplex DNA was followed using CD spectroscopy, DNA melting profiles, and fluorescent intercalator displacement (FID) assay. A new G-quadruplex binding assay based of quenching of Tb(III)-GQ luminescence was proposed and evaluated. All performed tests confirmed interactions of MCs with studied GQ structure. Binding affinities of MCs were appreciable (K-MC similar to 2-5 x 10(5) M-1). Higher concentration of MCs (the ratio of GQ:MC above 2.5) caused destabilization of tetraplex structure of GQ as evidenced by CD spectroscopy, melting temperatures, and Tb(III)-GQ luminescence quenching results. (C) 2015 Elsevier Inc. All rights reserved.
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