Article
Chemistry, Analytical
Dan Song, Xiangzhi Han, Wenjuan Xu, Jiayuan Liu, Yuxin Zhuo, Anna Zhu, Feng Long
Summary: A target nucleic acid amplification-free method called the CHANCE system was developed for rapid and sensitive detection of Escherichia coli O157:H7. The method showed a detection limit of 17.4 CFU/mL and could accurately detect E. coli O157:H7 within 50 minutes. It provides an ideal alternative for rapid and on-site detection of pathogenic bacteria in the environment and food.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Agriculture, Dairy & Animal Science
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Zhengzheng Wang, Qin Xiong, Hengyi Xu
Summary: In this study, a method for detecting viable E. coli O157:H7 in food products was established using an enhanced nucleic acid binding dye called improved propidium monoazide (PMAxx) coupled with rRAA assay. The PMAxx-rRAA assay showed high sensitivity and good specificity, and could resist interference from dead bacteria and nontarget bacteria.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Analytical
So-Young Lee, Se-Wook Oh
Summary: In this study, a rapid, sensitive, and visualized method of detecting E. coli O157:H7 in fresh products was developed, which rectified the common false-negative results and had a high detection sensitivity.
Article
Biochemical Research Methods
Dan Mu, Donggen Zhou, Guoyang Xie, Ju Liu, Qin Xiong, Xiaoyan Feng, Hengyi Xu
Summary: A quick and highly sensitive fluorescent probe-based RAA method was developed and successfully applied in detecting E.coli O157:H7, showing high sensitivity and feasibility for on-site testing in real samples.
MOLECULAR AND CELLULAR PROBES
(2021)
Article
Food Science & Technology
Shujuan Wang, Yiling Fan, Zhen Feng, Minghui Song, Qiongqiong Li, Bo Jiang, Feng Qin, Hao Liu, Lefu Lan, Meicheng Yang
Summary: The CRISPR-based diagnostic method utilizing the rfbE gene in the CRISPR/Cas12a system enables rapid detection of Escherichia coli O157:H7 with high sensitivity and specificity, with low detection limits for both DNA and bacterial concentrations. The target was successfully detected in ground beef samples after 4 hours of culture using Metal Organic Framework immunomagnetic beads enrichment when the initial bacterial inoculum was 14 CFU/mL.
Article
Microbiology
Xingxing Xiao, Ziqin Lin, Xianhui Huang, Jinfang Lu, Yan Zhou, Laibao Zheng, Yongliang Lou
Summary: A rapid and sensitive diagnostic method, RAA-CRISPR/Cas12a, has been developed for detecting V. vulnificus, showing great potential for early diagnosis of human vibriosis and on-site detection in aquaculture and food safety control.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Siyuan Liu, Dagang Tao, Yuying Liao, Yalan Yang, Shouzhang Sun, Yulan Zhao, Peng Yang, Yijie Tang, Bin Chen, Yonggang Liu, Shengsong Xie, Zhonglin Tang
Summary: This study developed a new method for highly sensitive and visual detection of PRRSV within 25 minutes by integrating the RT-RPA-Cas12a system with a single stranded DNA-fluorescently quenched reporter. The technique showed single copy sensitivity and no cross-reactivity with other porcine viruses. The results were highly accurate when compared with quantitative RT-qPCR, making it a promising tool for on-site detection of PRRSV.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Analytical
Lianyu Lu, Huimin Zhang, Fanghe Lin, Leiji Zhou, Zhi Zhu, Chaoyong Yang
Summary: The development of new diagnostic platforms for sensitive and rapid detection of bacterial pathogens is significant for improving precision medication for infectious disease and food safety control. The DMF-Cas12a method, which integrates the CRISPR/Cas12a system with a digital microfluidic chip, enables rapid, sensitive, and automated analysis of bacterial pathogens. The platform shows good potential in automated and highly sensitive pathogen detection for a wide variety of biological applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Parasitology
Fuchang Yu, Kaihui Zhang, Yilin Wang, Dongfang Li, Zhaohui Cui, Jianying Huang, Sumei Zhang, Xiaoying Li, Longxian Zhang
Summary: A new strategy for direct identification of a specific subtype of C. parvum has been successfully developed in this study, allowing for faster and more convenient diagnosis without the need for expensive equipment or highly trained operators.
PARASITES & VECTORS
(2021)
Article
Chemistry, Multidisciplinary
Gaihua Cao, Jiangbo Dong, Xiaolong Chen, Peng Lu, Yifan Xiong, Lan Peng, Jiawei Li, Danqun Huo, Changjun Hou
Summary: In this study, a strategy (MPT-Cas12a/13a) was developed that combines CRISPR/Cas12a and Cas13a for simultaneous detection of CaMV35S and T-nos. This strategy utilizes multiplex PCR and transcription to achieve simultaneous detection with different signals in the same space. The MPT-Cas12a/13a showed excellent sensitivity, with detection limits as low as 11 copies of T-nos and 13 copies of CaMV35S, and demonstrated outstanding specificity and anti-interference ability in actual sample analysis. Therefore, it has the potential to be used for the detection of GM crops.
CHEMICAL COMMUNICATIONS
(2022)
Article
Virology
Jianhui Zhao, Cailing Ao, Zhengwei Wan, Emmanuel Enoch Dzakah, Yuanhao Liang, Hongqing Lin, Haiying Wang, Shixing Tang
Summary: The study developed an integrated assay combining rRT-RAA and CRISPR Cas12a technologies for rapid and accurate detection of HIV-1 infection. It demonstrated high sensitivity and specificity in detecting HIV-1, showing potential as a reliable method for HIV-1 diagnosis.
Article
Parasitology
Peipei Cheng, Yuting Wu, Shuangshuang Guo, Xiaoyu Ma, Chenzhong Fei, Feiqun Xue, Chuangang Zhu, Mi Wang, Feng Gu
Summary: In this study, a method combining recombinase polymerase amplification (RPA) with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system was developed to detect Eimeria species in chicken fecal samples. The method showed high specificity and sensitivity, and enabled rapid detection of Eimeria species for on-site testing.
VETERINARY PARASITOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Shuai Zhi, Jinling Shen, Xingang Li, Yuan Jiang, Junxin Xue, Taisong Fang, Jin Xu, Xuan Wang, Yuhao Cao, Danting Yang, Zhiyuan Yao, Daniel Yu
Summary: A new RAA-exo-probe and RAA-CRISPR/Cas12a assays were developed for the rapid detection of Campylobacter jejuni in food samples. The assays showed high specificity and sensitivity, with a detection threshold lower than the traditional culture method. The simplicity and low equipment requirement make these assays suitable for field or poorly equipped labs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Liyun Lin, Guangcai Zha, Huagui Wei, Yuzhong Zheng, Peikui Yang, Yaqun Liu, Mouquan Liu, Zhonghe Wang, Xianghui Zou, Hui Zhu, Qiulan Luo, JinQuan Li, Min Lin
Summary: The study developed a detection method for Staphylococcus aureus in food using a combination of recombinase polymerase amplification (RPA) and CRISPR-Cas12a technology. The method demonstrated high sensitivity in detecting low quantities of S. aureus and proved feasible for use in both laboratory and field samples. This accurate and portable detection method has great potential in food safety applications.
Article
Forestry
Jing Zhou, Hanqian Dai, Tingting Dai, Tingli Liu
Summary: This study established a detection method for Phytophthora cambivora using a combination of whole-genome screening, recombinase polymerase amplification (RPA), and CRISPR/Cas12a. The RPA-CRISPR/Cas12a assay showed specific detection of P. cambivora isolates and could detect the presence of P. cambivora in apple fruits. Compared to conventional techniques, this method is faster, more cost-effective, and suitable for on-site detection at entry ports.