期刊
JOURNAL OF INFECTIOUS DISEASES
卷 215, 期 4, 页码 636-643出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiw607
关键词
Cryptosporidium; cryptosporidiosis; intestinal epithelium; parasitic infection; RNAs; nuclear delivery; gene transcription
资金
- National Institutes of Health [AI116323]
- Nebraska Stem Cell Research Program [LB606]
- Nebraska Department of Health & Human Services (DHHS) [LB595]
Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7_FLc_0990, involved heat-shock protein 70-mediated nuclear importing mechanism. Overexpression of Cdg7_FLc_0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells.
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