期刊
CARBOHYDRATE POLYMERS
卷 121, 期 -, 页码 18-26出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.12.007
关键词
Wheat bran; Arabinoxylan; Methanolysis; Enzymatic peeling; Immunolocalization; Confocal microscopy
资金
- COST Action FP0901, Hiller AG, Switzerland
- GoodMills Group GmbH, Austria
- Christian Doppler Forschungsgesellschaft, Austria
Arabinoxylan is one of the potential key products of a wheat bran based biorefinery. To develop a suitable process for the isolation of arabinoxylans, the effect of different processing approaches needs to be determined. In this work, chemical analysis was supplemented by immunolocalization of arabinoxylan by confocal microscopy, which proved valuable in the assessment of cell-structural changes occurring upon different chemical and mechanical bran treatments. The influences of acid, lye and hydrogen peroxide treatment, ball-milling, extrusion, fermentation and treatment with esterase, xylanase and a combination thereof were investigated. Extensive ball-milling showed the best selectivity for harvesting arabinoxylan. Chemical treatments gave the highest yields, but did so at the cost of selectivity. Fermentative and enzymatic treatments were hampered by coextraction of other compounds. (C) 2014 Elsevier Ltd. All rights reserved.
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